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Resveratrol zymophyte and application thereof

A technology of resveratrol and fermentation bacteria, which is applied in the direction of fermentation, bacteria, microorganisms, etc., can solve the problems of low extraction efficiency of plant extraction, less industrial application of biotechnology, and long synthesis route of chemical synthesis, so as to achieve easy separation and Purification and transformation are easy and the method is simple

Active Publication Date: 2017-08-01
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0004] The plant extraction method has low extraction efficiency, limited sources of raw materials, and high cost; the chemical synthesis method has a long synthesis route, high cost, and pollutes the environment; some other biotechnological applications are less industrialized and are currently in the experimental stage

Method used

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  • Resveratrol zymophyte and application thereof
  • Resveratrol zymophyte and application thereof
  • Resveratrol zymophyte and application thereof

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Embodiment 1

[0045] Screening, isolation and identification of strains

[0046] (1) Screening and isolation of bacterial strains

[0047] The fresh Polygonum cuspidatum roots used in the strain screening of the present invention were collected from Guangshui City, Suizhou City, Hubei Province.

[0048] ① Enrichment culture: Rinse the fresh Polygonum cuspidatum root from Guangshui City, Suizhou City, Hubei Province with 100mL of normal saline, soak and shake well, then take 1mL and add it to a triangular flask containing 50mL / 250mL enrichment medium. 220r / min, shake flask culture at 33~37℃ for 1~2d; the enrichment medium is the same as above.

[0049]②Preliminary screening: Gradiently dilute the enriched culture solution prepared in step ①, spread it on the primary screening plate, and culture it in a 37°C incubator for about 24 hours. Observe the growth of the colony, select the colony with a darker blue color and a larger area of ​​the blue part, transfer it to the primary screening pla...

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Abstract

The invention relates to a resveratrol zymophyte and application thereof. Classification of a bacterial strain is named as Bacillus-safensis, a storage unit is a general microorganism center of China Committee for Culture Collection of Microorganisms, preservation number is CGMCC No.13129, preservation address is No.3, No.1 Compound, Beichen West Road, Chaoyang District, Beijing, preservation date is October 21, 2016, and the application of the resveratrol zymophyte in fermented resveratrol is provided. A microbial transformation method is used for directly converting polydatin in polygonum cuspidatum crude medicinal materials to resveratrol. The method is simple, transformation process is easy to control, specificity of transformation reaction is stronger, separation and purification of products are convenient, yield and quality of resveratrol product are effectively improved, and added value of products is improved.

Description

technical field [0001] The invention belongs to the field of industrial microorganism application, and in particular relates to a resveratrol fermenting bacterium and its application. [0002] technical background [0003] Resveratrol (Resveratrol) (C 14 h 12 o 3 ) is a kind of phytoalexin (phytoalexin), which belongs to non-flavonoid polyphenolic compounds, and exists in many plants such as peanuts, grapes, knotweed, mulberry and so on. Resveratrol has anti-cancer, anti-oxidation, prevention and treatment of coronary heart disease, hyperlipidemia, immune regulation, anti-tumor, protection of cardiovascular system, whitening of external application, promotion of survival and proliferation of neural stem cells by activating Shh (sonichedgehog) signal, anti-depression and neuroprotective functions. [0004] The plant extraction method has low extraction efficiency, limited sources of raw materials, and high cost; the chemical synthesis method has a long synthesis route, hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/22C12R1/07
CPCC12N1/20C12P7/22C12N1/205C12R2001/07
Inventor 李玉冯薇邢雪岩秦慧民路福平
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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