Bacillus safensis and application thereof
A technology of bacillus saphobia and inoculum, which is applied to the function and the application field of inoculum in plant growth promotion, can solve the problems of obvious growth promotion effect and increase crop yield, etc., so as to improve disease resistance, increase yield, and improve effect of growth
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Embodiment 1
[0046] Example 1 Phosphorus dissolving function
[0047] Bacillus suffolus Bacillus safensis XWY06 strain was activated on LB medium.
[0048]The activated bacterial strain is inoculated into phosphate-dissolving medium (glucose 10.00g, (NH 4 ) 2 SO 4 0.50 g, MnSO 4 ·7H 2 O 0.3g, NaCl 0.3g, KCl 0.30g, FeSO 4 4H 2 O 0.036g, MnSO 4 4H 2 O 0.03g, distilled water 1000mL, pH 7.0. The inorganic phosphorus source is calcium phosphate, and the addition amount is 10g; the organic phosphorus source is calcium phytate, and the addition amount is 2g. Solid medium: add 1.5% agar powder in proportion).
[0049] Bacillus spp. was detected Bacillus safensis XWY06 has the ability to dissolve both inorganic phosphorus and organic phosphorus, and the diameter of the dissolution circle for dissolving inorganic phosphorus reaches 1.2cm, see figure 1 As shown, the diameter of the dissolution circle for dissolving organic phosphorus reaches 0.9cm, see figure 2 shown. Other phos...
Embodiment 2
[0052] Example 2 Protease production
[0053] Bacillus suffolus Bacillus safensis XWY06 strain was activated on LB medium.
[0054] Inoculate the activated strain into protease detection medium (tryptone 5.00 g, yeast extract 3.00 g, glucose 1.00 g, agar 15.00 g, distilled water 1000 mL, pH 7.0, autoclave at 121°C for 30 min. The sterilized When the detection medium is cooled to about 50°C, add 10% skimmed milk into the medium and mix well, then pour it into a petri dish, wait for it to cool down for later use), incubate at 28°C for 48 hours, and observe whether there is a dissolution circle.
[0055] The results showed that Bacillus spp. Bacillus safensis XWY06 has a good ability to dissolve protein, high protease production, and the diameter of its dissolution circle reaches 2.4cm, such as Figure 4 shown. Bacillus suffolus Bacillus safensis XWY06 high-yield protease, can be used for the extraction of protease, used in the food industry or washing industry and othe...
Embodiment 3
[0056] Example 3 Cellulase Production
[0057] Bacillus suffolus Bacillus safensis The XWY06 strain was activated on LB medium, and the activated strain was inoculated on the medium for cellulose detection (MgSO 4 ·7H 2 O 0.25g, K 2 HPO 4 0.50g, carboxymethylcellulose 1.88g, agar 15.0g, distilled water 1000mL, pH 7.0, autoclave at 121℃ for 30 min). Incubate at 28°C for 7 days. After 7 days, add 5 mL of 0.2 mg / mL Congo red staining solution to each plate, stain for 1 hour, discard the Congo red staining solution, add 1 M NaCl to wash for 1 hour, discard the washing solution, and observe the colonies Production of surrounding hydrolysis circles, the appearance of hydrolysis circles indicates the production of cellulase.
[0058] The results showed that Bacillus spp. Bacillus safensis The XWY06 strain has a good ability to dissolve cellulose, and the diameter of its dissolution circle reaches 4.3cm, such as Figure 5 shown. Bacillus suffolus Bacillus safensis XWY0...
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