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Bacillus safensis and application thereof

A technology of bacillus saphobia and inoculum, which is applied to the function and the application field of inoculum in plant growth promotion, can solve the problems of obvious growth promotion effect and increase crop yield, etc., so as to improve disease resistance, increase yield, and improve effect of growth

Active Publication Date: 2020-05-15
GENLIDUO BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing reports and published patents, Bacillus suffolus Bacillus safensis Although it also has the ability to decompose phosphorus and promote plant growth, there is no report that it has the ability to decompose inorganic phosphorus and organic phosphorus at the same time, and also has the ability to produce IAA, siderophilic, cellulase, and protease, and the growth-promoting effect is obvious.
Also do not see the report that utilizes phosphorus solubilizing microorganism to increase the soluble phosphorus content in the soil, and then increase the output of crops

Method used

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  • Bacillus safensis and application thereof
  • Bacillus safensis and application thereof
  • Bacillus safensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Phosphorus dissolving function

[0047] Bacillus suffolus Bacillus safensis XWY06 strain was activated on LB medium.

[0048]The activated bacterial strain is inoculated into phosphate-dissolving medium (glucose 10.00g, (NH 4 ) 2 SO 4 0.50 g, MnSO 4 ·7H 2 O 0.3g, NaCl 0.3g, KCl 0.30g, FeSO 4 4H 2 O 0.036g, MnSO 4 4H 2 O 0.03g, distilled water 1000mL, pH 7.0. The inorganic phosphorus source is calcium phosphate, and the addition amount is 10g; the organic phosphorus source is calcium phytate, and the addition amount is 2g. Solid medium: add 1.5% agar powder in proportion).

[0049] Bacillus spp. was detected Bacillus safensis XWY06 has the ability to dissolve both inorganic phosphorus and organic phosphorus, and the diameter of the dissolution circle for dissolving inorganic phosphorus reaches 1.2cm, see figure 1 As shown, the diameter of the dissolution circle for dissolving organic phosphorus reaches 0.9cm, see figure 2 shown. Other phos...

Embodiment 2

[0052] Example 2 Protease production

[0053] Bacillus suffolus Bacillus safensis XWY06 strain was activated on LB medium.

[0054] Inoculate the activated strain into protease detection medium (tryptone 5.00 g, yeast extract 3.00 g, glucose 1.00 g, agar 15.00 g, distilled water 1000 mL, pH 7.0, autoclave at 121°C for 30 min. The sterilized When the detection medium is cooled to about 50°C, add 10% skimmed milk into the medium and mix well, then pour it into a petri dish, wait for it to cool down for later use), incubate at 28°C for 48 hours, and observe whether there is a dissolution circle.

[0055] The results showed that Bacillus spp. Bacillus safensis XWY06 has a good ability to dissolve protein, high protease production, and the diameter of its dissolution circle reaches 2.4cm, such as Figure 4 shown. Bacillus suffolus Bacillus safensis XWY06 high-yield protease, can be used for the extraction of protease, used in the food industry or washing industry and othe...

Embodiment 3

[0056] Example 3 Cellulase Production

[0057] Bacillus suffolus Bacillus safensis The XWY06 strain was activated on LB medium, and the activated strain was inoculated on the medium for cellulose detection (MgSO 4 ·7H 2 O 0.25g, K 2 HPO 4 0.50g, carboxymethylcellulose 1.88g, agar 15.0g, distilled water 1000mL, pH 7.0, autoclave at 121℃ for 30 min). Incubate at 28°C for 7 days. After 7 days, add 5 mL of 0.2 mg / mL Congo red staining solution to each plate, stain for 1 hour, discard the Congo red staining solution, add 1 M NaCl to wash for 1 hour, discard the washing solution, and observe the colonies Production of surrounding hydrolysis circles, the appearance of hydrolysis circles indicates the production of cellulase.

[0058] The results showed that Bacillus spp. Bacillus safensis The XWY06 strain has a good ability to dissolve cellulose, and the diameter of its dissolution circle reaches 4.3cm, such as Figure 5 shown. Bacillus suffolus Bacillus safensis XWY0...

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Abstract

The invention relates to Bacillus safensis XWY06 which is preserved in China Center for Type Culture Collection with the preservation number of CCTCC No: M 2019818. The strain has stronger functions of decomposing inorganic phosphorus and organic phosphorus and can generate indoleacetic acid, cellulase, protease and siderophore. The invention also relates to a microbial inoculum containing the strain and application of the strain in promoting plant growth and improving the stress resistance of plants, such as disease resistance and drought resistance.

Description

technical field [0001] The invention relates to a strain of Bacillus suffolus and its application, in particular to the function of the strain of Bacillus suffolus and the application of its bacterial agent in plant growth promotion. Background technique [0002] Rhizosphere growth-promoting bacteria are defined as bacteria living freely in the soil, rhizosphere, root surface, and phyllosphere that are beneficial to plant growth under certain conditions. These bacteria are able to fix nitrogen, dissolve phosphorus and iron, and produce plant hormones such as auxins, gibberellins, cytokinins, and ethylene. In addition, they increase plant resistance to stress, including drought, high salinity, heavy metal poisoning, and pesticides. [0003] Phosphorus is the second largest nutrient element necessary for plant growth and is involved in multiple metabolisms of plants, including energy transfer, signal transfer, respiration, synthesis of macromolecules and photosynthesis. Howe...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12N9/54C12N9/42C12P17/10C12P1/04C12R1/07
CPCC12N1/20C05F11/08C12N9/54C12N9/2437C12Y302/01004C12P17/10C12P1/04C12R2001/07C12N1/205
Inventor 邢明振吕硕白军芳
Owner GENLIDUO BIO TECH CO LTD
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