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Microbial agent and preparation method and application thereof

A technology of microbial inoculum and bacterial count, applied in the field of microbial inoculum and its preparation, can solve the problems of low plant growth and seedling index and the like

Inactive Publication Date: 2015-04-29
BEIJING JUNO SCI AGRI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The purpose of the present invention is to overcome the problem of low plant strong seedling index existing in the current vegetable seedling raising process, to provide a microbial agent capable of improving the plant strong seedling index and its preparation method and application

Method used

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  • Microbial agent and preparation method and application thereof

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preparation example Construction

[0014] The present invention also provides a preparation method of the microbial agent, wherein the method comprises: inoculating Bacillus subtilis, Bacillus lincheniformis and Bacillus safensis in the culture medium to carry out to cultivate.

[0015] According to the present invention, the cultivation method includes: respectively cultivating Bacillus subtilis, Baclicus lincheniformis and Bacillus safensis in separate culture systems, and culturing the obtained Microorganisms are mixed in proportion. Preferably, by cultivating in separate culture systems and mixing after culturing, the total number of live bacteria per gram of microbial agent obtained is 2-10×10 7 indivual.

[0016] In a preferred embodiment, by cultivating in separate culture systems and mixing in proportion after culturing, the mixing conditions are not particularly limited, as long as the obtained microbial inoculum can meet the following conditions: Based on the total number of viable bacteria in the ...

Embodiment 1

[0025](1) Inoculate 5 parts by weight of Bacillus subtilis (ACCC01266) in 100 parts by weight of medium (glucose 5g, soybean meal 30g, peptone 2g, distilled water 1000ml, PH7.0-7.2), culture at 37°C, during the culture process Sampling was carried out and observed by microscope direct counting until the viable count of Bacillus subtilis was 2×10 7 per gram of culture medium.

[0026] (2) Inoculate 5 parts by weight of Bacillus licheniformis (ACCC01468) in 100 parts by weight of the medium (0.45g of corn steep liquor, 1.50g of peptone, 1:15 of bean cake powder soaking juice, 1.50g of glucose, pH 7.5), Cultivate at 37°C, take samples during the cultivation and observe by direct counting method under microscope until the number of viable bacteria of Bacillus licheniformis is 2×10 7 per gram of culture medium.

[0027] (3) In 100 parts by weight of medium (glucose 15g, starch 1g, bean cake powder 25g, manganese sulfate 1g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0...

Embodiment 2

[0030] (1) Inoculate 4 parts by weight of Bacillus subtilis (ACCC01266) in 100 parts by weight of medium (glucose 5g, soybean meal 30g, peptone 2g, distilled water 1000ml, pH7.0-7.2), and culture at 37°C. Sampling was carried out and observed by microscope direct counting method until the viable count of Bacillus subtilis was 8×10 7 per gram of culture medium.

[0031] (2) Inoculate 6 parts by weight of Bacillus licheniformis (ACCC01468) in 100 parts by weight of medium (0.45g of corn steep liquor, 1.50g of peptone, 1:15 of bean cake powder infusion, 1.50g of glucose, pH 7.5), Cultivate at 37°C, take samples during the cultivation and observe by direct counting method under microscope until the number of viable bacteria of Bacillus licheniformis is 8×10 7 per gram of culture medium.

[0032] (3) In 100 parts by weight of medium (glucose 15g, starch 1g, bean cake powder 25g, manganese sulfate 1g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.5g, yeast extract 0.2g,...

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Abstract

The invention provides a microbial agent. The microbial agent comprises a culture medium and thalli, wherein the thalli comprise Bacillus subtilis, Baclicus lincheniformis and Bacillus safensis. The microbial agent provided by the invention can obtain a good plant seedling index and further improve yield and quality of vegetables, thereby having broad application prospects in the field of vegetable seedling raising. In addition, by using the microbial agent provided by the invention, a plant rhizosphere microbial environment can be improved, and beneficial bacteria are increased, thereby inhibiting the growth of harmful bacteria and further improving the disease resistance of plants.

Description

technical field [0001] The present invention relates to a microbial bacterial agent and its preparation method and application; more specifically, it relates to a microbial bacterial agent and its preparation method, and also relates to the application of the biological bacterial agent in vegetable seedling cultivation. Background technique [0002] With the development of the world's agricultural economy, the agricultural planting structure is also undergoing corresponding adjustments, gradually transforming from traditional agriculture to modern agriculture, and the planting area of ​​vegetables is also increasing year by year, and seedling cultivation is a very important technical link in vegetable cultivation , Vegetable seedlings can not only make vegetables appear on the market earlier, but also increase vegetable yield and improve quality. [0003] At present, the problems in vegetable seedling cultivation are serious soil-borne diseases in vegetable seedling cultivat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00C12R1/125C12R1/10C12R1/07
CPCC12N1/20C05G3/00
Inventor 高红春朱昌雄顾金刚周艳丽
Owner BEIJING JUNO SCI AGRI TECH
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