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Microbial agent for promoting yield increase and disease resistance of potatoes and preparation method thereof

A kind of microbial agent and microbial technology, applied in the field of microbial agent, can solve the problems of discounting the effect of microbial agent, failure to prevent and control diseases, etc., and achieve the effect of increasing production

Active Publication Date: 2020-08-18
保罗蒂姆汉(潍坊)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some of the strains of the existing microbial agents have antagonism, and some have cooperation, and the unreasonable compounding will lead to a greatly reduced effect of the microbial agents and fail to prevent and control diseases.

Method used

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  • Microbial agent for promoting yield increase and disease resistance of potatoes and preparation method thereof
  • Microbial agent for promoting yield increase and disease resistance of potatoes and preparation method thereof
  • Microbial agent for promoting yield increase and disease resistance of potatoes and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 A kind of microbial bacterial agent that promotes potato production increase and disease resistance

[0057] In parts by weight, the microbial inoculant includes the following components:

[0058]

[0059] The Bacillus subtilis CICC21282 is a dry powder of dormant microorganisms, and the content of Bacillus subtilis spores in the dry powder is more than or equal to 10 11 piece / gram;

[0060] The Bacillus megaterium ACCC11099 is a dry powder of dormant microorganisms, and the content of Bacillus megaterium spores in the dry powder is ≥10 11 piece / gram;

[0061] The colloid bacillus ACCC10168 is a dormant microbial dry powder, and the colloid bacillus spore content in the dry powder is more than or equal to 10 10 piece / gram;

[0062] The Paenibacillus polymyxa ACCC10252 is a dry powder of dormant microorganisms, and the content of Paenibacillus polymyxa spores in the dry powder is ≥5×10 10 piece / gram;

[0063] The yeast ACCC20157 is a dry powder of dorm...

Embodiment 2

[0066] Embodiment 2 The preparation method of above-mentioned microbial bacterial agent

[0067] Include the following steps:

[0068] (1) Preparation of microbial dry powder of Bacillus subtilis CICC21282 dormant body

[0069] The activated Bacillus subtilis strains were inoculated into the seed tank with a volume ratio of 60% fermentation medium, the volume inoculation amount was 0.3%, the culture temperature was 37°C, the stirring speed of the seed tank was 230rpm, and the culture was 9h. The body reaches the logarithmic growth phase; the seed tank bacterial classification that will cultivate is inoculated into the fermentor that the volume ratio is equipped with in the fermentor of 60% fermentation medium, and the volume inoculation amount is 8%, and the formula of described seed tank and fermentor is identical, The formula of the medium is 30g / L of corn flour, 35g / L of soybean meal, 6g / L of sodium chloride, 5g / L of dipotassium hydrogen phosphate, 0.7g / L of magnesium sulf...

experiment example 3

[0086] The microbial agent of Example 1 was tested on potatoes, the test time was a growth cycle of potatoes, and the test site was Qianyizhuang Village, Dawu Town, Tengzhou City, Shandong Province.

[0087] 1. Soil for test: The soil type in this area belongs to sandy loamy tidal cinnamon soil, with flat and tidy terrain, good drainage and irrigation conditions, medium and uniform fertility, and no fertilizer test for previous crops.

[0088] 2. Varieties and cultivation methods tested: open-field mulched potatoes, Super Holland No. 15. The test was sown by the direct seeding method, sowing on February 23, and the seeds were germinated before sowing. The experimental field was raked after deep plowing, and then the germinated seeds were sown on demand after the row spacing was 70-80cm (the number of plants per mu was 3900- 4000 plants), ridges, herbicides and film mulching after sowing. According to the market price, harvesting will start on June 7th and end on June 11th.

...

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Abstract

The invention provides a microbial agent for promoting yield increase and disease resistance of potatoes, which comprises bacillus subtilis, bacillus megatherium, bacillus mucilaginosus, paenibacilluspolymyxa, saccharomycetes, bacillus safensis and lactobacillus plantarum. The invention also provides a preparation method of the microbial agent. The microbial agent is used for potato planting. Potato has developed roots, is high in growth vigor and has leaves which are large and dark green, the decay of potato seedlings is 7-10 days later than that of a control group, the surface gloss of fruits is good, bacterial wilt does not occur, and compared with an inactivated microbial agent or a microbial agent not applied, the microbial agent has the advantages that the weight of a single fruit is averagely increased by 0.8 g, and 0.7-1.2 potatoes per plant are averagely increased. The microbial agent is used for potato planting, the average acre yield reaches 4800-4850 kg, the yield is increased by 24-25% or above, and the incidence rate of bacterial wilt is 0%.

Description

technical field [0001] The invention relates to a microbial inoculum for promoting yield increase and disease resistance of potatoes and a preparation method thereof, belonging to the technical field of microbial inoculum. Background technique [0002] Potatoes are an important food crop in my country. The planting area and total output of potatoes in my country rank first in the world, but the yield per unit area is lower than the world average due to bottleneck factors such as diseases and insect pests. In recent years, soil-borne diseases caused by continuous cropping of potatoes tend to increase year by year, which directly affect the yield and quality of potatoes, and bring great economic losses to the potato industry. [0003] Potato diseased plants with bacterial wilt are slightly dwarfed, the leaves are light green or pale green, the lower leaves wilt first and then the whole plant droops, and it begins to recover sooner or later. After 4 to 5 days, the whole plant s...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12R1/125C12R1/11C12R1/07C12R1/12C12R1/645C12R1/25
CPCC12N1/20C12N1/16Y02W30/40
Inventor 郭芳先韩威华刘镇王于玺
Owner 保罗蒂姆汉(潍坊)生物科技有限公司
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