108 results about "Paenibacillus validus" patented technology

The invention relates to broad-spectrum compound microbial bacteria for preventing and treating vegetable fungal diseases, and belongs to the technical field of agricultural biological prevention and treatment. The broad-spectrum compound microbial bacteria is compounded from four microorganisms, namely bacillus subtilis BSD-2 ((i) Bacillus subtilis), brevibacillus brevis ch2 ((i) Brevibacillus brevis), bacillus amyloliquefaciens Y10 ((i) bacillus amyloliquefaciens) and paenibacillus polymyxa Y89 ((i) Paenibacillus polymyxa) according to a certain ratio. By adopting the compound bacteria, the vegetable fungal diseases of a greenhouse or a large field, for example, botrytis, leaf mold, downy mildew, powdery mildew, blight and the like can be effectively prevented and treated, the field efficacy can be up to over 70%, the yield is increased by 5-20%, and the broad-spectrum compound microbial bacteria are significant in economic, social and ecological benefits, and have broad popularization and application prospects.

Novel strains Paenibacillus sp. BS-0048, Paenibacillus sp. BS-0074, Paenibacillus polymyxa BS-0105 and Paenibacillus sp. BS-0277 and Fusaricidin A, Fusaricidin B and novel compounds 3 and 4 produced thereby have an activity of inducing resistance to plant diseases. Thus, they can protect plants from infections with fungi, bacteria, viruses and so on and, as a result, effectively control plant diseases.

The invention discloses a straw organic fertilizer and a preparation method thereof. The preparation method is to use a microbial inoculum used for degrading straw to ferment straw so as to obtain thefertilizer; the active ingredients of the microbial inoculum for degrading straw comprise Trichoderma viride, Trichoderma aureoviride and forage Paenibacillus pabuli. The colony formation unit ratioof Trichoderma viride to Trichoderma aureoviride and forage Paenibacillus pabuli in the microbial inoculum is (0.8-1.2):(0.8-1.2):(0.8-1.2). The fertilizer used for degrading straw can be used as a base fertilizer and can obviously improve the quality of vegetables.

This invention provides compositions of Bacillus strains and methods for using such compositions to inhibit the activity and / or growth of fungal pathogens of plants. In one embodiment, this invention provides a composition comprising Bacillus bacteria selected from the group consisting of Brevibacillus laterosporus strain CM-3, Brevibacillus laterosporus strain CM-33, Bacillus amyloliquefaciens BCM-CM5, Bacillus licheniformis ATCC-11946, Bacillus mojavensis BCM-01, Bacillus pumilus NRRL-1875, Bacillus subtilis 10 DSM-10, Bacillus subtilis NRRL-1650, Bacillus megaterium BCM-07, Paenibacillus polymyxa DSM-36, Paenibacillus chitinolyticus DSM-11030, and combinations thereof. In another embodiment, this invention provides a method for preparing a bacterial composition comprising one or more Bacillus strains by growing Bacillus strain bacteria until the bacteria form spores, collecting said spores, and formulating said composition.

The invention relates to a kind of Bacillus mucilaginosus and a culture method and culture medium thereof, belonging to the microbiological technical field. The Bacillus mucilaginosus P.muc3016 has the functions of nitrogen fixation, phosphorus-dissolving and potassium-releasing and belongs to the Paenibacillus group. The batch fermentation method is adopted to ferment and culture the Bacillus mucilaginosus and the concentrations of carbon source and dissolved oxygen are monitored and regulated. The culture medium contains carbon source, soybean meal powder, ammonium sulfate, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium sulfate and calcium carbonate, and the pH value of the culture medium is 7.5-7.0. The Bacillus mucilaginosus of the invention has three functions of nitrogen fixation, phosphorus-dissolving and potassium-releasing simultaneously, has higher function activity and can obviously increase the crop yield; and by adopting the culture method and culture medium of the invention, the fermentation density can be increased and the fermentation time can be shortened.

The invention provides a method for improving the biomass of chlorella in swine wastewater, belonging to the field of microbe application. Chlorella assigned with the accession number of CGMCC NO.9225 and paenibacillus elgii assigned with the accession number of CGMCC NO.7724 are co-cultured in the swine wastewater, the result shows that paenibacillus elgii can obviously improve the biomass of chlorella in the swine wastewater, and the promotion effect achieves 1.6 times. With the adoption of the method, the treatment problem of the swine wastewater is solved, and the environment pollution is reduced; due to the addition of paenibacillus elgii, the water body microenvironment in the swine wastewater is improved, the biomass of chlorella is obviously improved, further, the production cost of microalgae biodiesel is reduced, thus good economic benefit is obtained, and therefore, the method has a good market application prospect.

A portable, disposable device for the collection and bacteriological decomposition of liquid hydrocarbons including a pillow containing at least one member of the group consisting of (i) kenaf fibers / particles and (ii) a mixture of (a) natural, synthetic and / or oleophilic fibers / particles and (b)(i) one or more species of fungi selected from the group consisting of chaetonium indicum, chaetonium funicola, geotrichum candidum, macrophomina phaseolina, and trichoderma harziamum, and / or (b)(ii) one or more species of bacteria selected from the group consisting of bacillus subtillis, bacillus cereus sub group A and paenibacillus validus. The pillow may be quilted or otherwise constructed to define a plurality of chambers each containing the kenaf fibers / particles and / or the mixture of fibers / particles, fungi and / or bacteria. The device may further include a liquid impermeable pillow housing defining an opening to allow the liquid hydrocarbon to come into contact with the pillow. The pillow may define inter-connected channels for directing the flow of liquid hydrocarbons away from a contact point to evenly distribute the liquid hydrocarbons throughout the pillow. The upper and lower surfaces of the absorbent pillow may be constructed of different density fabric(s) and the pillow may define a sealed deflection area to further enhance the distribution of the liquid hydrocarbon throughout the pillow. A method of using the device includes the steps of: obtaining the device; placing the device proximate to a source of the liquid hydrocarbon and bringing the liquid hydrocarbon into contact with the device.

The present invention provides, in part, a Paenibacillus sp. isolate, designated Paenibacillus polymyxa JB05-01-1, as well as an anti-microbial agent obtained from the bacterium or cell culture supernatant thereof. Compositions, methods and uses are also provided.

The invention provides a complex flora for preventing and treating peanut root rot and an application thereof, and belongs to the technical field of soil-borne disease prevention and treatment. The complex flora comprises pantoea, pseudobacillus, enterobacter, paenibacillus, sporosarcina, bacillus and pseudomonas aeruginosa. The pantoea, the pseudobacillus, the enterobacter, the paenibacillus, thesporosarcina, the bacillus and the pseudomonas aeruginosa in the complex flora have a synergistic interaction effect on inhibition of pathogenic fungi; compared with a single strain, the complex flora has the advantages that the rhizosphere colonization capacity is obviously improved, and the rhizosphere micro-ecological environment can be effectively optimized. The complex flora is inoculated topeanut roots, so that the peanut roots can be effectively prevented from being infected by fusarium oxysporum, and the health of the peanut roots is protected.

The invention discloses a paenibacillus damxungensis sp.nov. CGMCC No.8333 chymosin and a preparation method. The method comprises the following steps: (1) centrifuging fermentation broth of which the preservation number is CGMCC No.8333 to obtain fermenting supernate; (2) mixing the fermenting supernate and ammonium sulfate until the saturability of the ammonium sulfate in the fermenting liquid is 50-75% so as to obtain a mixed solution, standing the mixed solution for 0-5 hours at 2-6 DEG C, and centrifugally collecting a precipitate; (3) dissolving the precipitate centrifugally to obtain the supernate. According to the method provided by the invention, the steps of a preparation process of chymosin can be greatly simplified; furthermore, compared with a method of extracting chymosin by using ethanol, the production cost can be greatly saved, the method is convenient and effective to operate, safe and reliable to use, and is beneficial for industrial production.

The invention relates to the field of microorganism preparations, and provides a chitosan and microorganism compound preparation. The chitosan and microorganism compound preparation is prepared from chitosan, lactobacilli and compound bacilli, wherein the compound bacilli comprise bacillus licheniformis, bacillus subtilis and paenibacillus polymyxa, the number of the effective living bacteria is 1.5-2.5 hundred millions / ml, and the pH value is 6.8-7.0. In the chitosan and microorganism compound preparation, by compounding the chitosan, the lactobacilli and the bacilli, the contents of ammonianitrogen, phosphorus and nitrate in a breeding water body can be effectively reduced, so that the water quality of the breeding water body is improved; the chitosan and microorganism compound preparation can also effectively promote soft-shelled turtle growth, and weight gain is improved by at least 15% compared with that of a control group without the chitosan and microorganism compound preparation; meanwhile, the orrho-immunity protein content and the antioxidant capacity of soft-shelled turtles are improved at different degrees, so that the immunocompetence of the soft-shelled turtles is significantly improved.

The invention discloses a process using Chongqing Fengjie Yunyan 87 flower buds as raw materials to extract essential oil and application of the essential oil. The process includes: adding distilled water into smashed Chongqing Fengjie Yunyan 87 flower buds according to the material-liquid ratio of 1:10-1:30, adding cellulase accounting for 2-10% of the weight of the tobacco flower buds, adding sodium chloride accounting for 5-25% of total weight, performing ultrasonic treatment for 10-40 minutes, and distilling with steam for 2-6 hours to prepare the flower bud essential oil. The flower bud essential oil prepared by the process has the advantages that the essential oil is capable of well eliminating OH free radicals and O2- free radicals and is a good antioxidant substance; meanwhile, the essential oil has certain antibacterial activity against escherichia coli, bacillus subtilis and paenibacillus polymyxa and is a good antibacterial agent. The Chongqing Fengjie Yunyan 87 flower bud essential oil prepared by the process can be used as an antioxidant agent and the antibacterial agent.

The invention relates to paenibacillus Lzh-N1 capable of preventing and treating pear brown spots and application of a complex microbial agent thereof. The paenibacillus Lzh-N1 is preserved in the common microbial center of the China microbial culture preservation management committee with the preservation number of CGMCC No.13455, the preservation date is December 15, 2016, and the address of thepreservation institution is No.3, Yard 1, Beichenxi Road, Chaoyang District, Beijing. The invention further discloses application of the paenibacillus Lzh-N1 and microbial fertilizer of a complex microbial agent of the paenibacillus Lzh-N1 and streptomyces globosus to prevention and treatment of pear brown spots. The paenibacillus Lzh-N1 can effectively prevent pear brown spots and improve the quality and output of pears, and has the effects of nontoxicity, high efficiency and non-environment pollution.

The invention provides an organic degrading bacterium resistant to cadmium and lead ions. The organic degrading bacterium is named as paenibacillus sp GRINM L4 in a way of classification, and is preserved in China General Microbiological Culture Collection Center (CGMCC); the address of the preservation unit is institute of microbiology, Chinese academy of sciences, No. 3, yard 1, Beichen west road, Chaoyang district, Beijing; the preservation data is September 29th, 2016, and the preservation number is CGMCC No. 13066. The strain can efficiently remove organic matters in wastewater rich in heavy metal ions, and has a large application space in the field of COD removal of heavy metal-rich flotation wastewater and power plant wastewater in the future, thus having a great market developmentprospect.

The invention relates to the technical field of microbial agents, and in particular to a microbial preparation as well as a preparation method and application of the microbial preparation. The microbial preparation is prepared by adopting and fermenting paenibacillus mucilaginosus, and a fermented culture solution contains shrimp and crab shells, collagen, sodium chloride, amino acid, carbohydrate and compound fertilizer, so that the microbial preparation has the function of inhibiting harmful germs; according to the microbial preparation disclosed by the embodiment of the invention, spray application and root irrigation can be carried out on rose and lycium chinense which suffer from a root rot disease and banana which suffers from a panama disease, and a result shows that the microbial preparation provided by the invention has a good prevention and treatment function on the root rot disease of the rose and the lycium chinense and the prevention effect can be up to 85.3 percent and 69.0 percent; the microbial preparation has a good prevention and treatment function on a blight disease of the banana, and the prevention effect can be up to 85.0 percent.

The invention discloses a paenibacillus polymyxa neopullulanase gene as well as a method for producing neopullulanase through cloning and expression and fermentation. The method comprises the following steps: by selecting Paenibacillus polymyxa CFCC10334 as an original strain and taking escherichia coli BL21 as a genetic engineering host bacterium, directionally inserting a neopullulanase gene Npu in the paenibacillus polymyxa into an expression plasmid according to the physical map of the escherichia coli expression vector plasmid, transforming escherichia coli BL21, acquiring the neopullulanase gene when an expression product ENpu5 has normal biological activity, through cloning and transformation, performing induction expression in escherichia coli, screening positive expressor, measuring the enzyme activity, thereby obtaining a genetic engineering strain with relatively high enzyme activity, and performing fermentation production, thereby obtaining a neopullulanase product. The stability and the activity of enzyme are improved, and the production cost is lowered.

The invention discloses a composite anti-biological inoculant prepared by compounding fermentation liquor of bacillus pumilus NF2 and fermentation liquor of paenibacillus polymyxa DL1, as well as a preparation method of the composite anti-biological inoculant and application of the inoculant in aspects of controlling pepper phytophthora blight, improving disease resistance of pepper and increasingpepper yield. The composite anti-biological inoculant combines the anti-biological characteristics of the bacillus pumilus NF2 and paenibacillus polymyxa DL1, the antibacterial spectrum is widened, multiple antibacterial mechanisms are combined, the micro-ecological balance of soil is fully coordinated, the adaptability of the strain to the environment is enhanced, the synergistic disease resistance advantages among the strains are achieved for a long time, and the composite anti-biological inoculant has an excellent anti-biological effect compared with a single strain of paenibacillus polymyxa DL1. In addition, the composite anti-biological inoculant is capable of regulating the micro-ecological environment of plant rhizosphere soil, and producing system disease resistance by plant induction, so that the plant disease resistance can be improved, the comprehensive control effects of promoting growth and resisting diseases are finally achieved, and the aims of improving the quality, increasing yield and increasing income of the pepper can be achieved.

The invention discloses paenibacillus sp. CL01 and an application thereof. The paenibacillus sp. CL01 is preserved in China General Microbiological Culture Collection Center on June 28, 2020, and thepreservation number of the strain is CGMCC No.20161. The strain is wide in antibacterial spectrum and strong in antibacterial ability, has a strong inhibition effect on common pathogenic fungi on peartrees such as colletotrichum gloeosporioides, alternaria alternata, alternaria alternata and valsa ceratosperma, and also has a strong antagonistic effect on phytopathogens such as rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum and phytopathogen. The biocontrol microbial inoculum prepared from the strain has a relatively high biocontrol effect on fungal diseases of fruits and leaves on pear trees. The composition can replace or reduce the use of chemical pesticides, improves the safety of food and ecological environment, has good economic and social benefits, and has a very wide application prospect in biological prevention and control of plant diseases.

The invention relates to the field of microbial agents, and particularly discloses a saline alkali soil remediation microbial agent and a preparation method thereof.The saline alkali soil remediationmicrobial agent is prepared from, by weight, 25-30 parts of microbial agent carriers, 10-15 parts of composite microbial agents and 25-30 parts of biochemical organic acid, the complex microbial inoculant comprises Bacillus amyloliquefaciens, Paenibacillus mucilaginosus, Bacillus megaterium and Trichoderma harzianum, wherein the Bacillus amyloliquefaciens, the Paenibacillus mucilaginosus, the Bacillus megaterium and the Trichoderma harzianum are mixed according to a mass ratio of (1-2): (1-2): (1-2): 5. The microbial inoculum carrier is attapulgite, and the biochemical organic acid comprises biochemical fulvic acid and brown humic acid which are mixed in equal mass. The components of the microbial agent are optimized, the defects of a single-strain microbial agent are overcome, meanwhile,attapulgite is adopted as a matrix, organic acid is used as an auxiliary improvement material in an auxiliary mode, the saline-alkali soil remediation rate is improved, the improvement service life ofthe saline-alkali soil remediation agent is prolonged, and finally the saline-alkali soil is effectively improved.

The invention belongs to the technical field of biology, and particularly relates to a high-density mixed fermentation preparation method of composite bacillus microbial agent. Rich nutrient components of the dried distillers' grains are utilized, enzymatic hydrolysate generated by alkaline protease enzymolysis of the dried distillers' grains serves as a fermentation culture medium solvent, and high-density mixed fermentation of the biocontrol probiotic paenibacillus polymyxa and the bacillus firmus is achieved by controlling the fermentation pH, performing fed-batch cultivation and maintaining the dissolved oxygen amount. The effective viable count of the paenibacillus polymyxa in the fermentation liquor can reach 1.2 to 1.5* 10<10>CFU / mL, and the effective viable count of the bacillus firmus can reach 8.0 to 9.0*10<9> CFU / mL, so that the fermentation time is shortened, and the production efficiency is improved. The number of effective viable bacteria or viable spores in the compositebacillus microbial agent prepared from the dry vinasse enzymolysis residues can reach 3.0-6.6*10<11>CFU / g, full utilization of the dry vinasse raw material is achieved, and the production cost of themicrobial agent is reduced.

The invention discloses bacillus for preventing and treating root rot of paris polyphylla, and a preparation method and application thereof. A paenibacillus polymyxa strain HJ-2 is adopted to infect the fusarium zoospores, then germination of fusarium spore is further inhibited, and harm of fusarium to rhizome parts of the paris polyphylla is reduced, so that harm of root rot to the paris polyphylla is reduced, the yield of the paris polyphylla is ensured, and economic loss of pesticide farmers is reduced. In addition, the paenibacillus polymyxa strain HJ-2 is used for biological prevention, so that pesticide residues are not generated, and the bacillus provided by the invention has the characteristics of greenness and recyclability, good prevention and control effect, avoidance of pesticide residue pollution and the like.

The invention discloses a preparation method of paenibacillus bacteriocin. The preparation method comprises the following steps: (1) inoculating the paenibacillus into a culture medium for culturing the paenibacillus, culturing in oscillating and fermenting to obtain fermented liquor, centrifuging the obtained fermented liquor, and taking the supernatant; (2) mixing the obtained supernatant with ethanol in volume ratio of (2:1)-(4:1), standing obtained mixed liquor for 12-36 hours at temperature of 2-10 DEG C, centrifuging the mixed liquor to collect precipitate, removing a solvent to obtain the paenibacillus bacteriocin. Compared with the traditional bacteriocin, the bacteriocin prepared through the method disclosed by the invention is more stable, the bacteriocin preparation can be directly applied to the production of foods, thereby achieving the aim of inhibiting the proliferation of putrefying bacteria and prolonging shelf life of the food, and then the application cost of the food preservative is reduced.

The invention discloses a Paenibacillus sp. bacteriocin extract, and a preparation method and application thereof. The preparation method comprises the following steps: (1) inoculating Paenibacillus sp. into a tomato juice culture medium, and carrying out shake culture to obtain a fermentation liquid; and (2) centrifuging the fermentation liquid obtained in the step (1), taking the supernatant, ultrafiltering, taking the low-molecular part, and carrying out freeze-drying. The preparation of the bacteriocin extract at present is dependent on the chemically synthesized culture medium. The research of the bacteriocin extract prepared from tomato juice is not reported yet. The tomato juice culture medium is used as a natural fermentation culture medium in preparing the bacteriocin extract for the first time, and thus, the product has higher food safety. By using the tomato as the raw material, the Paenibacillus sp. bacteriocin extract has the advantages of wide sources and low cost. The fermentation strain is the single Paenibacillus sp. strain. The technique is beneficial to standardization of the product quality and cost control on industrial large-scale production.

The invention belongs to the technical field of agricultural microorganisms, and particularly provides a special compound microbial agent for tobacco seedling raising and a preparation method and application of the special compound microbial agent. The special compound microbial agent is formed by compounding specific bacillus coagulans, bacillus tequila and paenibacillus polymyxa in proportion, wherein the bacillus coagulans can produce IAA at high yield and promote tobacco growth. The bacillus tequila and the paenibacillus polymyxa can effectively inhibit common bacterial wilt and black shank of tobaccos, B06 can promote the antibacterial ability of the bacillus tequila and the paenibacillus polymyxa, and the three microorganisms cooperate for use, so that the growth of the tobaccos canbe promoted, the yield of the tobaccos can be increased, diseases can be prevented and resisted, and the tobacco leaf quality can be improved.

The invention provides a microbial agent for promoting yield increase and disease resistance of potatoes, which comprises bacillus subtilis, bacillus megatherium, bacillus mucilaginosus, paenibacilluspolymyxa, saccharomycetes, bacillus safensis and lactobacillus plantarum. The invention also provides a preparation method of the microbial agent. The microbial agent is used for potato planting. Potato has developed roots, is high in growth vigor and has leaves which are large and dark green, the decay of potato seedlings is 7-10 days later than that of a control group, the surface gloss of fruits is good, bacterial wilt does not occur, and compared with an inactivated microbial agent or a microbial agent not applied, the microbial agent has the advantages that the weight of a single fruit is averagely increased by 0.8 g, and 0.7-1.2 potatoes per plant are averagely increased. The microbial agent is used for potato planting, the average acre yield reaches 4800-4850 kg, the yield is increased by 24-25% or above, and the incidence rate of bacterial wilt is 0%.

The invention discloses a high-yield beta-glucanase strain capable of effectively degrading salecan. The classification name of the high-yield beta-glucanase strain is paenibacillussp.S09, the collection registration number is CCTCC NO: M2012196, the collection date is May 30, 2012, the collection unit is China Center for Type Culture Collection (CCTCC) with the address of Collection Center of Wuhan University, Wuchang Luojiashan, Wuhan, Hubei province, and the registration number of 16SrRNA gene sequence of the beta-glucanase strain on Genbank is JQ945736. The invention also discloses a method for producing beta-glucanase by using the paenibacillussp.S09 and a crude enzyme preparation obtained according to the method. The enzyme preparation has high enzyme activity of laminarin enzyme and lichenase, and has wide application prospects in the fields of animal husbandry, brewing industry, biological control and the like.

The invention relates to a special fertilizer for cadmium reduction of ligusticum wallichii and an application method of the special fertilizer; the special fertilizer comprises a soil conditioner anda microbial agent, and the soil conditioner comprises a Womeike soil conditioner; the microbial agent comprises the components: bacillus subtilis, bacillus megatherium, bacillus laterosporus, paenibacillus polymyxa and trichoderma harzianum. The application method comprises the steps: applying the special cadmium-reducing fertilizer for ligusticum wallichii as a base fertilizer, spreading one week before ligusticum wallichii is planted, and covering with soil; the special fertilizer for cadmium reduction of ligusticum wallichii is used for reducing heavy metal absorption of the ligusticum wallichii medicinal material. The method solves the problems that existing ligusticum wallichii genuine production area soil is polluted by heavy metals, the cadmium content in ligusticum wallichii rhizomes seriously exceeds the standard due to the strong enrichment effect of ligusticum wallichii on cadmium in cultivation soil, and the drug use safety and market requirements of ligusticum wallichii are greatly influenced.

The invention relates to the technical field of paenibacillus and application thereof, and provides paenibacillus with a yield increasing effect and a preparation method and application thereof. The paenibacillus is prepared into a microbial agent, and the microbial agent can effectively promote plant yield increasing. The invention further provides a method for preparing the microbial agent. Thedefects in the prior art are overcome, and the problems that in existing fertilization and use of insecticides and bactericides for crop yield increasing, soil is easily damaged, meanwhile, chemical agents can also be left on crops, and the yield increasing effect is affected are solved. The paenibacillus is adopted and prepared into the microbial agent, the yield increasing effect on various crops can be improved, the microbial agent is easy to prepare, short in fermentation time and convenient to apply, meanwhile, the yield increasing efficiency is high, and the advantages of increasing thecrop yield, improving the crop quality, improving the soil, protecting the ecological environment, reducing pollution and the like are achieved.