Alkaline soil remediation microbial inoculum and preparation method thereof

A technology for repairing bacterial agents and saline-alkali soil, which is applied in the direction of soil conditioning materials, chemical instruments and methods, and other chemical processes. The effect of reducing soil alkalinity

Active Publication Date: 2021-03-16
JIANGSU ZHIFENG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defects of low remediation rate and low durability of the alkaline soil remediation bacterial agent in the process of alkaline soil remediation, in the first aspect, the application provides an alkaline soil remediation bacterial agent, which adopts the following technical scheme:

Method used

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  • Alkaline soil remediation microbial inoculum and preparation method thereof
  • Alkaline soil remediation microbial inoculum and preparation method thereof
  • Alkaline soil remediation microbial inoculum and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Cultivation and collection of Trichoderma harzianum: Take 400g wood, 100g bran and 200g soybean meal, dry them naturally and pulverize them, sterilize them with ultraviolet light and collect the pulverized particles to obtain a solid-state fermentation substrate; then weigh 4500mL deionized water and 300g starch respectively Granules and 100g of sodium nitrate are placed in a mixer, stirred and dispersed at room temperature and the fermentation nutrient solution is collected; the fermentation nutrient solution is sprayed onto the surface of the solid-state fermentation substrate, and after the moisture content of the solid-state fermentation substrate is controlled to 55%, Trichoderma harzianum is pressed by 7.5g / kg inoculum mass concentration, dispersely inoculated into solid-state fermentation substrate, and cultured in a fresh-keeping bag filled with small holes, and fermented and cultivated at 26°C for 140 hours. The ratio is 1:8, the air-dried fermented product is ...

Embodiment 2

[0039] Cultivation and collection of Trichoderma harzianum: 450g of wood, 120g of bran and 250g of soybean meal were naturally dried and crushed, sterilized by ultraviolet light, and the crushed particles were collected to obtain a solid-state fermentation substrate; then weighed 4700mL of deionized water and 400g of starch Granules and 150g of sodium nitrate are placed in a mixer, stirred and dispersed at room temperature and the fermentation nutrient solution is collected; the fermentation nutrient solution is sprayed onto the surface of the solid-state fermentation substrate, and after the moisture content of the solid-state fermentation substrate is controlled to 55%, Trichoderma harzianum is pressed by 7.5g / kg inoculum mass concentration, dispersely inoculated into solid-state fermentation substrate, and cultured in a fresh-keeping bag filled with small holes, and fermented and cultivated at 27°C for 145h. The ratio is 1:9, the air-dried fermented product is mixed with st...

Embodiment 3

[0047] Cultivation and collection of Trichoderma harzianum: 500g of wood, 150g of bran and 300g of soybean meal were naturally dried and crushed, treated with ultraviolet light and collected to obtain a solid-state fermentation substrate; then weighed 5000mL of deionized water and 300g of Starch granules and 100g of sodium nitrate are placed in a mixer, stirred and dispersed at room temperature, and the fermentation nutrient solution is collected; the fermentation nutrient solution is sprayed onto the surface of the solid-state fermentation substrate, and after the moisture content of the solid-state fermentation substrate is controlled to 60%, Trichoderma harzianum is mixed with 7.5 g / kg inoculum mass concentration, disperse inoculation into solid-state fermentation substrate, and pack into a fresh-keeping bag filled with small holes for culture, and ferment and cultivate at 28°C for 150h. Stir and mix 100g of air-dried fermented product with 1000mL of sterile water and filter...

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Abstract

The invention relates to the field of microbial agents, and particularly discloses a saline alkali soil remediation microbial agent and a preparation method thereof.The saline alkali soil remediationmicrobial agent is prepared from, by weight, 25-30 parts of microbial agent carriers, 10-15 parts of composite microbial agents and 25-30 parts of biochemical organic acid, the complex microbial inoculant comprises Bacillus amyloliquefaciens, Paenibacillus mucilaginosus, Bacillus megaterium and Trichoderma harzianum, wherein the Bacillus amyloliquefaciens, the Paenibacillus mucilaginosus, the Bacillus megaterium and the Trichoderma harzianum are mixed according to a mass ratio of (1-2): (1-2): (1-2): 5. The microbial inoculum carrier is attapulgite, and the biochemical organic acid comprises biochemical fulvic acid and brown humic acid which are mixed in equal mass. The components of the microbial agent are optimized, the defects of a single-strain microbial agent are overcome, meanwhile,attapulgite is adopted as a matrix, organic acid is used as an auxiliary improvement material in an auxiliary mode, the saline-alkali soil remediation rate is improved, the improvement service life ofthe saline-alkali soil remediation agent is prolonged, and finally the saline-alkali soil is effectively improved.

Description

technical field [0001] The present application relates to the field of microbial bacterial agents, more specifically, it relates to an alkaline soil remediation bacterial agent and a preparation method thereof. Background technique [0002] Alkaline soil is a soil that is formed under the combined action of various factors such as human activities and the natural environment. It contains a large amount of soluble salts, has a salinized layer or an alkalized layer, and makes plants unable to grow normally. . In this type of soil, the process of exchangeable sodium continuously entering the soil colloid, especially the organic-mineral absorptive complex, is also called the sodicization process. When the calcium ions of the soil colloid are all replaced by sodium ions, the saturated sodium The ions are desorbed from the colloid and enter the soil solution, resulting in high salinity and high alkalinity in the alkaline soil. The soil humus is easily leached and the soil structu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40C09K109/00
CPCC09K17/40C09K2109/00
Inventor 韩秋平李霞
Owner JIANGSU ZHIFENG BIOLOGICAL TECH CO LTD
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