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Organic degrading bacterium resistant to cadmium and lead ions, and application of organic degrading bacterium

A lead ion, organic matter technology, applied in the field of applied microbiology, can solve the problems of death, loss of organic matter degradation ability, and low growth activity.

Active Publication Date: 2018-11-23
有研资源环境技术研究院(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most organic matter-degrading microorganisms are difficult to tolerate high concentrations of toxic heavy metal ions in industrial wastewater, have low growth activity or even gradually die, and lose the ability to degrade organic matter. Therefore, it is very important to screen organic matter-degrading microorganisms that can tolerate heavy metal ions

Method used

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  • Organic degrading bacterium resistant to cadmium and lead ions, and application of organic degrading bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Resistance to cadmium, enrichment isolation and purification of organic matter degrading bacterial strains of lead ions

[0016] (1) culture medium

[0017] Solid medium: 1g / L glucose, 1g / L yeast extract, 300mg / L mineral processing black powder, 300mg / L mineral processing xanthate, 0.3g / L magnesium sulfate heptahydrate, 0.5g / L ammonium sulfate, 100mg / L lead acetate , 100mg / L cadmium sulfate, 0.3g / L potassium chloride, 0.002g / L manganese sulfate, 20g agar, 6mL 0.4% bromophenol blue, pH7.0~7.5.

[0018] Liquid medium: 1g / L glucose, 1g / L yeast extract, 300mg / L mineral processing black powder, 300mg / L mineral processing xanthate, 0.3g / L magnesium sulfate heptahydrate, 0.5g / L ammonium sulfate, 100mg / L lead acetate , 100mg / L cadmium sulfate, 0.3g / L potassium chloride, 0.002g / L manganese sulfate, pH7.0~7.5.

[0019] The above-mentioned medium was sterilized in an autoclave at 115°C for 30 minutes for later use.

[0020] (2) Enrichment and isolation of strains

[...

Embodiment 2

[0024] Example 2 Screening, Purification and Identification of Organic Matter Degrading Strains

[0025] Observe the colony form on the solid medium plate, and observe the form of the bacterial strain with an optical microscope, and obtain the preliminary identification result of the desired bacterial strain according to the morphological characteristics of the obtained bacterial strain "Comparison of Fungal Identification Handbook (Written by Wei Jingchao)". Use a toothpick to stick the preliminarily identified single colony into a test tube containing 3ml of liquid medium, and culture it in a shaker at 160r / min for three days at 31°C to obtain a single pure bacterium. Then use PCR to amplify the 16S rDNA sequence of the strain and perform sequencing analysis. gov / Blast.cgi?PROGRAM=blastn&PAGE_TYPE=Blast Search&LINK_LOC=blasthome) for comparison, the comparison result is Paenibacillus sp, which belongs to Paenibacillus, named GRINM L4.

Embodiment 3

[0026] Example 3 Detection of Organic Matter Degrading Bacteria Strain's High Ability to Remove Organic Matter Rich in Heavy Metal Ion Wastewater

[0027] 1. Shake flask culture in liquid medium

[0028] The initially screened GRINM L4 strain was inoculated into a 250mL Erlenmeyer flask containing 100mL of mineral processing wastewater rich in heavy metal ions at an inoculation amount of 3%. Incubate on a shaker for 2 days, take samples before and after treatment, blank sample before treatment, and test sample after treatment, and measure solution COD.

[0029] The waste water contains 53.8mg / L cadmium and 63.7mg / L lead. The standard limit value of the basic items of the surface water environmental quality standard stipulates that the limit value of cadmium in class V surface water (maximum limit) is 0.01mg / L, and the limit value of lead is 0.01mg / L. 0.1mg / L, the cadmium and lead content in the waste water used in this embodiment is far greater than the surface water standard...

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Abstract

The invention provides an organic degrading bacterium resistant to cadmium and lead ions. The organic degrading bacterium is named as paenibacillus sp GRINM L4 in a way of classification, and is preserved in China General Microbiological Culture Collection Center (CGMCC); the address of the preservation unit is institute of microbiology, Chinese academy of sciences, No. 3, yard 1, Beichen west road, Chaoyang district, Beijing; the preservation data is September 29th, 2016, and the preservation number is CGMCC No. 13066. The strain can efficiently remove organic matters in wastewater rich in heavy metal ions, and has a large application space in the field of COD removal of heavy metal-rich flotation wastewater and power plant wastewater in the future, thus having a great market developmentprospect.

Description

technical field [0001] The invention belongs to the field of applied microbiology and relates to a bacterial strain for degrading organic matter, in particular to a cadmium- and lead ion-resistant organic matter-degrading bacterial strain. Background technique [0002] Organic wastewater generally refers to wastewater rich in carbohydrates, fats, proteins, cellulose and other organic substances discharged from industries such as papermaking, leather and food. If the wastewater is discharged directly, it will cause serious pollution. There are many simple organic wastewater treatment methods of this type, and the technology is relatively mature. However, the non-ferrous metal industry and the power industry will produce wastewater containing high concentrations of heavy metal ions and high concentrations of organic matter, which is relatively difficult to treat. Some enterprises discharge a large amount of organic wastewater containing heavy metals, and the main indicators ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/01C02F101/20C02F101/30
CPCC02F3/34C02F2101/20C02F2101/30C12N1/02C12N1/20C12N1/205C12R2001/01
Inventor 张明江刘兴宇李益斌温建康
Owner 有研资源环境技术研究院(北京)有限公司
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