Paenibacillus with yield increasing effect and preparation method and application thereof
A technology of Paenibacillus and Bacillus, applied in the field of Paenibacillus and its preparation, can solve the problems of easily damaged soil, chemical agent residues on crops, and affecting the effect of increasing production, so as to reduce pollution, improve crop quality, and increase production. Effect
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[0032] A method for preparing a crop yield-increasing bacterial agent, comprising the steps of:
[0033] Step 1: Streak the strain on LB solid medium for activation culture at 20-37°C.
[0034] Step 2: Pick a single colony and inoculate it into LB liquid medium, and culture it on a shaker at 20-37°C and 100-300r / min for 1-3 days.
[0035] Step 3: The fermented strain liquid fermentation broth can be used directly after dilution.
Embodiment 1
[0037] Experimental materials: ginger, bacterial agents of the above strains, LB medium
[0038] Experimental settings: water control group, 1ml LB blank control group, 1ml of the above bacterial agent treatment group. Each group was repeated 5 times, and the inoculum was watered once every 2 weeks.
[0039] Experimental process: In this experimental case, after pouring the fungus 10 times, wait for the ginger to mature, which lasted 4 months, pull it out and clean it, measure the yield of each plant, and calculate the average plant yield of each group.
[0040] In this case, there was no difference in the growth and development of the three groups, but when the average yield per plant was finally measured, the group that applied 1ml of the above bacterial agent had a 45.29% increase in production compared to the 1ml LB blank control group; The yield of the control group increased by 37.97%.
Embodiment 2
[0042] Experimental materials: eggplant, bacterial agents of the above strains, LB medium
[0043] Experimental settings: water control group, 10ml LB blank control group, 10ml of the above bacterial agent treatment group. Each group was repeated 5 times, and the inoculum was watered once every 2 weeks.
[0044] Experimental process: In this experimental case, the fungus was poured 10 times, which lasted 3 months, and the fruit was picked 4 times, and all the fruits were picked each time, the yield of each plant was measured, and the average plant yield of each group was calculated.
[0045] In this case, there was no difference in the growth and development of the three groups, but when the average yield per plant was finally measured, the group that applied 1ml of the above bacterial agent had a 45.29% increase in production compared to the 1ml LB blank control group; The yield of the control group increased by 37.97%.
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