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Method for preparing trichoderma solid spawn through trichoderma direct fermentation crop straw and prepared product

A technology for solid bacteria and crop straws, applied in microorganism-based methods, biochemical equipment and methods, products, etc., can solve the problems of difficulty in rapid reproduction, slow growth and reproduction of Trichoderma, and increased production costs of enterprises.

Active Publication Date: 2016-05-18
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many problems often occur in factory production, and it is difficult to achieve the high-density solid fermentation target of Trichoderma. For example, it is difficult to achieve complete sterilization of solid materials for large-scale solid fermentation, even if the solid materials are sterilized thoroughly, and the solid fermentation process It is also difficult to control the entry of miscellaneous bacteria in the air into solid materials, and the rapid growth of miscellaneous bacteria often makes it difficult for the inoculated Trichoderma strains to grow rapidly and reproduce to 10 9 More than 1 spore / g; Secondly, the sterilization of such a large amount of solid materials in factory production greatly increases the production cost of the enterprise, coupled with the cost of strictly controlling the air purification degree of the fermentation space during the solid fermentation process, it becomes economically feasible for Trichoderma solid fermentation thirdly, because the best nutritional formula that can make Trichoderma grow and reproduce quickly has not been found yet, the growth and reproduction speed of Trichoderma in solid materials is relatively slow, and it is difficult to become a dominant population and rapidly reproduce and suppress Bacterial growth

Method used

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  • Method for preparing trichoderma solid spawn through trichoderma direct fermentation crop straw and prepared product
  • Method for preparing trichoderma solid spawn through trichoderma direct fermentation crop straw and prepared product
  • Method for preparing trichoderma solid spawn through trichoderma direct fermentation crop straw and prepared product

Examples

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Effect test

Embodiment 1

[0057] A method for preparing Trichoderma solid strains by directly fermenting crop stalks with Trichoderma, adding amino acid diluent to sun-dried corn stalks with a fineness of 3 mm (moisture content 15%, organic carbon content of 66%), and amino acid diluent The dosage is to add 200ml of amino acid diluent per 100g of dried straw (equivalent to adding 10ml of amino acid hydrolyzate per 100g of dried straw), adjust the initial pH to 3.5, age for 12 hours, and then inoculate with 10% Harmony Trichoderma SQR-T037 liquid strain, the water content of the heap is 60-70% at this time, the heap is solidly fermented, the temperature in the fermentation room is 30±2°C, the indoor air humidity is 65%±5%, after 9 days of fermentation Obtain Trichoderma solid strain, wherein the content of Trichoderma reaches 5.8×10 9 individual / g, the content of miscellaneous bacteria is 5.1×10 6 pcs / g.

Embodiment 2

[0059] In the decomposed pig manure compost, add 1% Trichoderma solid bacterial classification prepared according to the method of the present invention to carry out solid fermentation, turn over 1-2 times every day in the solid fermentation process, make the solid fermentation temperature not exceed 60 ℃, ferment 6 -End after 7 days, get Trichoderma bio-organic fertilizer. Field experiments on the biological effect of Chinese cabbage were carried out in Nanjing, Jiangsu and Nantong, Jiangsu. The field results showed that, under the condition of equal nutrient input, the yield of Trichoderma bio-organic fertilizer was 23.4% higher than that of chemical fertilizer on Nanjing yellow-brown soil vegetable soil. (See Table 5), increase production by 9.3% (see Table 5) than amino acid organic fertilizer; Increase production by 10.5% (see Table 6). The above field results show that the yield-increasing effect of Trichoderma bio-organic fertilizer is more significant than that of che...

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Abstract

The invention discloses a method for preparing trichoderma solid spawn through trichoderma direct fermentation crop straw and a prepared product. The method comprises the steps that liquid amino acid is added into the crop straw to adjust the initial pH to be 3.0-4.0, and then trichoderma liquid spawn is inoculated into the crop straw for solid fermentation to obtain the trichoderma solid spawn. The acidity of the solid fermentation material for enabling trichoderma to rapidly grow and breed and restraining growth of other bacterium indexes is created, meanwhile, the best trichoderma nutrition formulation is screened out, and the cheap trichoderma solid spawn fermentation patented technology is creatively built; the trichoderma spore content in the trichoderma bio-organic fertilizer per gram can reach 5*10<7> or above after 1% of the trichoderma solid spawn prepared through the method is added into the mature compost, and the yield-increasing effect is obvious after the trichoderma bio-organic fertilizer product is applied.

Description

technical field [0001] The invention belongs to the field of strain fermentation, and relates to a method for preparing solid Trichoderma strains by directly fermenting crop stalks with Trichoderma and a prepared product. Background technique [0002] Trichoderma spp. is widely used in the prevention and treatment of soil-borne fusarium wilt, and is considered to be the most ideal biocontrol fungus. It is widely distributed, easy to separate and cultivate, and can inhibit the growth of various soil-borne pathogens. It also has a variety of biological control mechanisms, including heavy parasitism, antibiosis, competition, and induced resistance. In particular, Trichoderma harcii has shown a good effect on preventing and controlling soil-borne fusarium wilt or Verticillium wilt on various crops such as cucumbers, watermelons, bananas, yams, eggplants, and cotton. The colonization of Trichoderma strains in the rhizosphere can form a competitive relationship with Fusarium oxys...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05F11/08C12R1/885C05G3/60
CPCC05F11/08C12N1/14C05F9/04C05G3/60Y02P20/133Y02P20/145Y02A40/20
Inventor 沈其荣黄启为刘东阳李荣
Owner NANJING AGRICULTURAL UNIVERSITY
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