Method for preparing trichoderma solid spawn through trichoderma direct fermentation crop straw and prepared product
A technology for solid bacteria and crop straws, applied in microorganism-based methods, biochemical equipment and methods, products, etc., can solve the problems of difficulty in rapid reproduction, slow growth and reproduction of Trichoderma, and increased production costs of enterprises.
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Embodiment 1
[0057] A method for preparing Trichoderma solid strains by directly fermenting crop stalks with Trichoderma, adding amino acid diluent to sun-dried corn stalks with a fineness of 3 mm (moisture content 15%, organic carbon content of 66%), and amino acid diluent The dosage is to add 200ml of amino acid diluent per 100g of dried straw (equivalent to adding 10ml of amino acid hydrolyzate per 100g of dried straw), adjust the initial pH to 3.5, age for 12 hours, and then inoculate with 10% Harmony Trichoderma SQR-T037 liquid strain, the water content of the heap is 60-70% at this time, the heap is solidly fermented, the temperature in the fermentation room is 30±2°C, the indoor air humidity is 65%±5%, after 9 days of fermentation Obtain Trichoderma solid strain, wherein the content of Trichoderma reaches 5.8×10 9 individual / g, the content of miscellaneous bacteria is 5.1×10 6 pcs / g.
Embodiment 2
[0059] In the decomposed pig manure compost, add 1% Trichoderma solid bacterial classification prepared according to the method of the present invention to carry out solid fermentation, turn over 1-2 times every day in the solid fermentation process, make the solid fermentation temperature not exceed 60 ℃, ferment 6 -End after 7 days, get Trichoderma bio-organic fertilizer. Field experiments on the biological effect of Chinese cabbage were carried out in Nanjing, Jiangsu and Nantong, Jiangsu. The field results showed that, under the condition of equal nutrient input, the yield of Trichoderma bio-organic fertilizer was 23.4% higher than that of chemical fertilizer on Nanjing yellow-brown soil vegetable soil. (See Table 5), increase production by 9.3% (see Table 5) than amino acid organic fertilizer; Increase production by 10.5% (see Table 6). The above field results show that the yield-increasing effect of Trichoderma bio-organic fertilizer is more significant than that of che...
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