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Special compound microbial agent for tobacco seedling raising and preparation method and application of special compound microbial agent

A technology of composite microbial agent and microbial agent, which is applied in the field of agricultural microorganisms, can solve problems such as short shelf life, low product activity, and unstable effect, and achieve the effects of long shelf life, low cost, and improved quality of tobacco leaves

Active Publication Date: 2020-11-17
北京恩格兰环境技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, some microbial fertilizer products have been used in tobacco seedling cultivation, but there are problems such as low product activity, short shelf life, and unstable effect.

Method used

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  • Special compound microbial agent for tobacco seedling raising and preparation method and application of special compound microbial agent
  • Special compound microbial agent for tobacco seedling raising and preparation method and application of special compound microbial agent
  • Special compound microbial agent for tobacco seedling raising and preparation method and application of special compound microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Screening of Bacillus coagulans B06:

[0037] 1) Screening of wild bacteria with high yield of IAA

[0038] Medium:

[0039]MRS liquid medium: glucose 20g / L, peptone 10g / L, yeast extract 5g / L, beef extract powder 10g / L, diammonium citrate 2g / L, sodium acetate 5g / L, Tween 80 1g / L, Dipotassium hydrogen phosphate 2g / L, pH6.5.

[0040] YPD liquid medium: glucose 20g / L, peptone 20g / L, yeast extract powder 10g / L, pH natural.

[0041] For solid medium, 1.5% agar was added on the basis of liquid medium, and all medium was sterilized at 115°C for 20 minutes.

[0042] The root soil of tea trees was collected from the tea garden of Huazhong Agricultural University, and 1g of soil samples were put into MRS medium, cultured at 37°C for 7 days, and water bathed at 80°C for 15min, then 0.1ml was transferred to the solid plate of MRS medium, and cultured at 37°C. After the colonies grow out, the colonies are randomly picked, purified, numbered and stored. The strains were transfer...

Embodiment 2

[0071] Screening of Bacillus tequila KQ22 against R. solanacearum

[0072] Medium:

[0073] LB liquid medium: tryptone 10g / L, yeast extract powder 5g / L, sodium chloride 10g / L, pH natural.

[0074] YPD liquid medium: glucose 20g / L, peptone 20g / L, yeast extract powder 10g / L, pH natural.

[0075] For solid medium, 1.5% agar was added on the basis of liquid medium, and all medium was sterilized at 115°C for 20 minutes. Multiple soil samples were collected from the tobacco planting site in Laifeng County, Enshi Prefecture, Hubei Province, and each 5g soil sample was placed in 300ml YPD liquid medium for static culture at 37°C for 3 days. Take 0.1ml of each dilution to coat the YPD solid plate and incubate at 37°C. After the colonies grew out, the colonies were randomly selected for streaking and isolation, and the purified strains were numbered and stored. At the same time, these strains were transferred to YPD liquid medium and cultured at 37°C for 48 hours. The inhibition zone...

Embodiment 3

[0079] The preparation method of special composite microbial agent for tobacco seedling cultivation:

[0080] In this method, bacillus coagulans B06 and bacillus tequila KQ22 are respectively cultured and dried in respective appropriate mediums, and then compounded with Paenibacillus polymyxa powder according to the number of viable bacteria in a certain proportion.

[0081] The Paenibacillus polymyxa is purchased from Wuhan Konuo Biotechnology Co., Ltd., which is referred to as P2 in the present invention, and the number of live bacteria in the bacterial powder is 5 billion CFU / g.

[0082] 1) The culture medium of Bacillus coagulans B06 is composed of brown sugar, yeast extract powder, bran, light calcium carbonate and water in a mass ratio of 4:0.5:100:2:80. Calcium carbonate, brown sugar and yeast extract are dissolved, and then mixed with wheat bran evenly. After sterilizing at 121°C for 30 minutes, cool down to below 50°C and then inoculate 5% Bacillus coagulans B06 seed...

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Abstract

The invention belongs to the technical field of agricultural microorganisms, and particularly provides a special compound microbial agent for tobacco seedling raising and a preparation method and application of the special compound microbial agent. The special compound microbial agent is formed by compounding specific bacillus coagulans, bacillus tequila and paenibacillus polymyxa in proportion, wherein the bacillus coagulans can produce IAA at high yield and promote tobacco growth. The bacillus tequila and the paenibacillus polymyxa can effectively inhibit common bacterial wilt and black shank of tobaccos, B06 can promote the antibacterial ability of the bacillus tequila and the paenibacillus polymyxa, and the three microorganisms cooperate for use, so that the growth of the tobaccos canbe promoted, the yield of the tobaccos can be increased, diseases can be prevented and resisted, and the tobacco leaf quality can be improved.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a special composite microbial agent for raising tobacco seedlings and its preparation method and application. Background technique [0002] Tobacco is an important economic crop in my country, mainly concentrated in Yunnan, Guizhou, Chongqing, Hubei, Hunan and other provinces, and occupies an important position in the national economy. In tobacco production, seedling cultivation is an important link in tobacco leaf production. The current tobacco leaf production adopts the production method of raising seedlings and transplanting, that is, intensively cultivating tobacco seedlings, and transplanting them in the field after they grow into seedlings. The main advantages of this method are: economical use of land, increase the multiple cropping index; concentrate the tobacco seedlings in an environment with superior light, temperature and water, survi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C12R1/07C12R1/12
CPCC12N1/20A01N63/22
Inventor 贺福平何志群
Owner 北京恩格兰环境技术有限责任公司
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