35 results about "Paenibacillus sp." patented technology

The invention discloses a preparation method of a directed vat set chymosin preparation and a product and an application thereof. The preparation method comprises the following steps: culturing paenibacillus (Paenibacillus sp.) at 25-35 DEG C by using a skimmed milk culture medium, shake culturing for 24-120 hours to obtain fermentation broth, centrifuging the fermentation broth and extracting the supernatant to obtain the directed vat set chymosin preparation. According to the preparation method, the skimmed milk is innovatively used as a base material for fermentation, since being a milk product as well, the obtained chymosin can be directly applied to the preparation process of cheese, compared with the traditional chymosin preparation method, the method is used for greatly simplifying the application steps of the chymosin preparation, retaining the chymosin activity to the uttermost and completely avoiding the enzyme activity loss of chymosin in the extraction process, so as to effectively reduce the production and application costs of the chymosin preparation.

The invention discloses a method for simultaneously removing USEPA PAHs in plant bodies by utilizing compound PAHs degrading bacteria. Multiple functional bacteria are prepared into a microbial compound functional fungicide, a plant absorption promoter, cytokinin 6-BA or KT, auxin 2,4-D and cysteine are added to prepare a degradation agent, then the degradation agent is inoculated in the plant bodies, and the PAHs content in the plant body is reduced; the compound fungicide is prepared from 8 PAHs degrading bacteria different in degradation spectrum, wherein the degrading bacteria are Sphingobiumsp sp. RS1 and RS2, mycobacterium sp. Pyr9, 033, Diaphorobacter sp. Phe15 and Massilia sp. Pn2, Paenibacillus sp. Phe3, Pseudomonas sp. Ph6 respectively. The acenaphthylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo[a] anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenzo[a,h] anthracene, benzo[ghi]indene and [1,2,3-cd] pyrene in each plant body in a contaminated area can be effectively removed and degraded, the removal rate of PAHs is high, and the method has the advantages of high efficiency, environmental protection and easy operation.

The invention discloses a dietary supplement with a bacteriostatic action and a preparation method and application thereof. The method for preparing the dietary supplement with the bacteriostatic action comprises the following steps: (1) inoculating Paenibacillus (Paenibacillus sp.) in a tomato juice culture medium, shaking and performing fermentation culturing, thereby obtaining the fermentation liquor; and (2) centrifuging the fermentation liquor obtained in the step (1), taking the supernatant, and performing freeze drying, thereby obtaining the product. According to the dietary supplement with the bacteriostatic action disclosed by the invention, the adopted raw material refer to tomato juice during preparation, and the source is natural, so the dietary supplement has high food safety. Moreover, the preparation method of the dietary supplement is simple in process, and only three steps, namely fermenting, centrifuging and freeze drying are needed so as to finish the method, and the possible pollution problem brought by the traditional complicated process is greatly reduced. The obtained dietary supplement is obvious in bacteriostatic effect and stable in bacteriostatic action.

The invention discloses biological fermentation wheat bran with an antibacterial effect and a preparation method and application of the biological fermentation wheat bran. The preparation method of the biological fermentation wheat bran with the antibacterial effect comprises the following steps: (1) inoculating Paenibacillus sp. into a wheat bran culture medium and performing aerobic culture to obtain a fermented matter; and (2) drying the fermented matter obtained in the step (1) for preparation. The preparation method disclosed by the invention adopts a solid-state fermentation method, has the advantages of single strain and raw material, simple process and low cost, and is conductive to standardization of fermentation wheat bran quality and cost control of industrial large-scale production. The biological fermentation wheat bran with the antibacterial effect, prepared by the preparation method disclosed by the invention, can effectively relieve and even fundamentally change the present situation of abuse of antibiotics in existing animal cultivation industry after being used in animal feed industry.

The invention discloses a paenibacillus sp. strain HB172198; the systematic name of the strain HB172198 is Paenibacillus sp.; the preservation number is CGMCC No.15412; the preservation date is 5th March, 2018; the preservation institution is China General Microbiological Culture Collection Center, CGMCC; and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3building No.1 yard Beichengxi road, Chaoyang district, Beijing. The invention also discloses an alginate lyase and a preparation method thereof, and an application of the paenibacillus sp. strain andthe alginate lyase in degradation of brown algae.

The invention discloses a bacteriocin crude extract with bacteriostatic action, and a preparation method and application thereof. The preparation method of the bacteriocin crude extract comprises the following steps: (1) inoculating Paenibacillus sp. into a culture medium for culturing Paenibacillus sp., and carrying out shake fermentation and culture to obtain a fermentation liquid; and (2) centrifuging the fermentation liquid obtained in the step (1), taking the precipitate, adding acetone, evenly mixing, leaching, centrifuging, taking the supernatant, and removing acetone. The method solves the problem of narrow bacteriocin sources at present. Compared with the traditional bacteriocin, the bacteriocin crude extract prepared by the method has stabler properties, and thus, has higher food safety. The bacteriocin crude extract can be directly used for food production, thereby achieving the goals of inhibiting putrefying bacteria and prolonging the shelf life of food, and further lowering the application cost.

The invention relates to the field of microbial fertilizer, and specifically relates to a composite microelement fertilizer containing chlorella and paenibacillus. The composite microelement fertilizer is prepared by mixing chlorella sp. and paenibacillus sp. together. The composite microelement fertilizer can prominently promote the growth of plants, and is capable of enhancing the disease resistant performance of plants; moreover, the using amount o f chemical fertilizer and chemical pesticide is effectively reduced, the quality of agricultural products is obviously improved, and thus the composite microelement fertilizer can be used and popularized in agricultural production.

The invention discloses radiation-resistant type paenibacillus sp. KH9 and an application thereof in a biological antitranspirant. A soil sample collected in arid desert region around the Lop Nor area in Xinjiang Province is separated, screened and cultured, and the radiation-resistant type paenibacillus sp. KH9 with relatively high polysaccharide production capacity and the function of reducing plant transpiration is obtained and has the CGMCC No.12799. The radiation-resistant type paenibacillus sp. KH9 has remarkable physiological-biochemical characteristic difference and molecular level difference by comparison with common paenibacillus sp.; when the screened-out radiation-resistant type paenibacillus sp. KH9 and the antitranspirant are compounded and used for drought resistance, biological films are formed on leaves of crops, transpiration of the crops can be reduced effectively, the photosynthesis function is improved, the chlorophyll content is increased by 4.50%, and the radiation-resistant type paenibacillus sp. KH9 have higher growth promoting capacity, osmotic adjustment ability and active oxygen defense capacity for crops and can reduce damage to the crops under drought stress.

Provided are a microbial agent capable of making a side slope and bulkhead vegetation community free of artificial maintenance and a construction method thereof. The microbial agent is prepared through the steps of preparation of fermentation raw materials, smashing of the fermentation raw materials, liquefaction, sterilization, fermentation, desiccation and packaging, and desiccation and packaging are conducted on the raw materials which are subjected to fermentation. The microbial agent comprises various fungi such as paenibacillus sp., bacillus amyloliquefaciens, arthrobacter humicola, pseudomonas graminis, phanerochaete chrysosporium, trichoderma longibrachiatum, aspergillus niger, rhodotorula mucilaginosa, sporobolomyces and clitocybe geotropa. According to the microbial agent free of artificial maintenance for the side slope and bulkhead vegetation community, by means of a nutrient substance circulation theory of dry branches and fallen leaves, the microbial agent is added to a target vegetation community in one time, in situ degradation is conducted on the dry branches and fallen leaves to activate microorganisms, in an ecological system, a new system which has the functions of matter circulation, energy flow, soil fertility increase and vegetation community update and succession is formed among the dry branches and fallen leaves, soil and vegetation, therefore, artificial cultivation of the side slope and bulkhead vegetation community is not needed, and plants grow vigorously.

The invention discloses a preparation method and a product of paenibacillus chymosin. The preparation method comprises the following steps: (1) inoculating paenibacillus sp. in a culture medium for culturing paenibacillus sp. to carry out oscillatory fermentation culture and centrifuging the obtained fermentation liquor to get the liquid supernatant; (2) mixing the obtained liquid supernatant with ethyl alcohol and centrifuging to obtain the precipitate; and (3) dissolving the obtained precipitate by water or PBS, centrifuging to get the liquid supernatant and carrying out freeze-drying to obtain the paenibacillus chymosin. The preparation method is capable of greatly simplifying the preparation steps of the chymosin and effectively reducing the production cost of the chymosin under the premise of improving the specific activity and the recovery rate of the chymosin. The chymosin prepared by the preparation method has relatively high specific activity and is capable of sufficiently satisfying the industrial production demand of the chymosin.

The invention discloses a paenibacillus sp. bacteriocin crude extract as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) inoculating paenibacillus sp. into a culture medium for culturing the paenibacillus sp., shocking and carrying out fermentation cultivation to obtain fermentation liquor, and centrifuging the obtained fermentation liquor so as to take liquid supernatant; and (2) adding ammonium sulfate to the obtained liquid supernatant, centrifuging and collecting precipitates which are separated out when the saturation of the ammonium sulfate is 30-70%, dissolving the obtained precipitates and then dialyzing, and carrying out freeze drying to obtain the paenibacillus sp. bacteriocin crude extract. Raw materials for preparing the paenibacillus sp. bacteriocin crude extract are bran, wide in source and low in cost, and a fermentative strain is a paenibacillus sp. single strain; and the technology facilitates the standardization of product quality and the cost control of industrialized mass production. The paenibacillus sp. bacteriocin crude extract can be directly applied to food production, thus achieving the purposes of inhibiting the proliferation of putrefying bacteria and prolonging the shelf period of food and reducing the application cost.

The invention discloses a microbial flocculating agent generated by paenibacillus and applications thereof. The microbial flocculating agent is generated by paenibacillus sp. LX03, and is prepared by the following steps: inoculating paenibacillus sp. LX03 into a fermentation culture medium, carrying out cultivation on a shaking table for 2 to 3 days, subjecting the fermentation broth to centrifugation to remove bacterial cells, adding ethanol, which is 2 to 3 times the volume of the fermentation broth, to precipitate extracellular products, drying the extracellular products, and adding water to dissolve extracellular products to obtain the microbial flocculating agent. When the microbial flocculating agent is compounded with iron ions and aluminum ions, a good flocculating effect can be achieved. When the microbial flocculating agent is used with an inorganic flocculating agent namely polymeric aluminum chloride, the using amount of polymeric aluminum chloride is largely reduced, and a better flocculating effect is formed in a short time. The microbial flocculating agent can be used as a good water treatment flocculating agent and flocculating aid, and can be widely used in the fields such as household wastewater treatment, industrial wastewater treatment, and the like.

The present invention provides, in part, a Paenibacillus sp. isolate, designated Paenibacillus polymyxa JB05-01-1, as well as an anti-microbial agent obtained from the bacterium or cell culture supernatant thereof. Compositions, methods and uses are also provided.

The invention especially relates to a fermentation medium for Paenibacillus sp. 1-49 and an optimization method thereof, belonging to the technical fields of fermentation mediums and optimization of fermentation mediums. The fermentation medium is prepared from the following raw materials by mass: 15 to 20 g of bean cake powder, 5 to 8 g of maize starch, 6 to 10 g of cane sugar, 5 to 8 g of yeast powder, 0.2 to 0.5 g of MgSO<4>.7H<2>O, 1 to 3 g of CaCO<3>, 0.01 to 0.03 g of MgSO<4>.H<2>O, 0.25 to 0.75 g of KH<2>PO<4> and 1000 mL of distilled water with a pH value of 7.0 to 7.5. The optimization method comprises the following steps: determination of a nitrogen source; determination of a carbon source; determination of a carbon-nitrogen ratio; and determination of a pH value. The optimized fermentation medium for Paenibacillus sp. 1-49 in the invention overcomes the problem of low spore output of Paenibacillus sp., greatly increases spore output, enhances the application effect of Paenibacillus sp. 1-49 and effectively reduces production cost.

The invention discloses growth promoting rhizobacteria PHE-2 with PAHs degradation capability. The rhizobacteria is classified and named as Paenibacillus sp., and is preserved in the China General Microbiological Culture Collection Center on August 26th, 2014 with a preservation number of CGMCC No.1.12900. A functional microbial inoculum prepared from the strain PHE-2 has a phenanthrene degradation rate of 95% or above within 5 days under the condition of shake flask culture, wherein the initial concentration of the phenanthrene is 100 mg x L<-1>. Meanwhile, the strain PHE-2 can also rapidly degrade anthracene, pyrene and chrysene. The strain PHE-2 has good root surface film forming ability, has various growth promoting properties, can generate indoleacetic acid and iron carrier, and has phosphorus dissolving ability. The strain PHE-2 can be directly used for reducing the pollution risk of phenanthrene in PAHs-polluted water, soil and plants, and can obviously promote the growth of plants.

The invention discloses a strain with high cellulose enzyme activity and application and obtaining methods thereof. The strain is named as paennibacillus (Panenibacillus sp.WH1) which is stored at a typical culture preservation centre in China in Luojia Hill, Wuchang, Wuhan City, Hubei Province, the serial number of registration of preservation is CCTCC No: M2014236, and preservation data is May 29, 2014. The paenibacillus sp WH1 with high cellulose enzyme activity is obtained by being separated from pulp sludge, so that higher cellulose enzyme activity is achieved; the paenibacillus sp WH1 can be widely used in food industry, brewing industry, paper industry, feed industry, textile industry and biomass conversion, biomass material conversion efficiency can be improved, and cellulose conversion cost can be lowered.

The invention belongs to the application of a microbial fermentation technology, in particular to a fermented feed additive and a preparation method of the fermented feed additive. The preparation method includes the steps as follows: (1) adding rice bran and water in an organic matter which serves as a culture nutrient, leading the water content to be 50-60%, thus obtaining the organic matter of the culture nutrient; and (2) adding microorganism with the CCTCC (China Center For Type Culture Collection) NO: M2010334 and CCTCC name: Thermophilic Bacillus IBALAKI-02 (Paenibacillus sp.IBALAKI-02) in the organic matter which serves as the culture nutrient to ferment, wherein the weight ratio of the Thermophilic Bacillus to the organic matter of the culture nutrient is 1:500000. The fermented feed additive can strengthen the anti-disease capability of poultry, reduce the usage of antibiotics, simultaneously enhance the stomach-intestine movement and the digestion to food of the poultry so as to enable the poultry to grow fast, and reduce the feeding cost. In addition, when the culture nutrient is prepared, waste during the processing of food or plate waste behind the ambry can be used, therefore the purposes of utilizing waste and reducing production cost are achieved.

The present disclosure relates to a novel strain capable of saccharifying and fermenting biomass-derived cellulose and a recombinant strain thereof with improved biomass saccharification capability. The present disclosure also relates to a method for producing a material useful as a bioenergy source material such as ethanol, acetic acid, formic acid, etc. using the strain or the recombinant strain. The strain or the recombinant strain may be usefully used in bioenergy industry.

The invention discloses a Paenibacillus and application thereof to removal of gas containing sulfur dioxide. The strain is specifically Paenibacillus sp. IB1, and the preservation number is CGMCC No.10775. The Paenibacillus sp. IB1 has very strong acid resistance, can continuously and effectively purify sulfur dioxide in an environment with very strong acidity (pH2), can adapt to high-temperature conditions, can still grow well at 50-65 DEG C and can effectively remove sulfur dioxide in high-temperature waste gas. Bacterium agents prepared by using the strain and microbial active fillers prepared by loading bacterium suspension on porous fillers can be used for treating waste gas containing sulfur dioxide, reach 90-100 percent in sulfur dioxide degrading efficiency, and has the characteristics of short startup time and high loading capacity.

The invention relates to the technical field of microbial inoculations, in particular to a composite microbial inoculation and its preparation method and application. The compound microbial agent includes Paenibacillus nitrogen-fixing 1-49, Bacillus megaterium B16 and Bacillus amyloliquefaciens T600; the preparation method of the compound microbial agent includes seed culture, fermentation culture, and the optimal combination of different bacterial liquid additions in the fermentation culture is passed through positive The cross-test confirmed; the compound microbial agent is applied to promote the growth of sugarcane and improve the agricultural nitrogen-saving effect. The composite microbial bacterial agent of the present invention has a remarkable nitrogen-saving effect and has a huge market advantage; the present invention optimizes the addition amount of different bacterial solutions for liquid fermentation through orthogonal experiments, improves the spore production of nitrogen-fixing bacteria, and greatly improves the quality of production , can effectively reduce production costs; the three strains of the present invention are spores, which have the advantages of long storage time and long shelf life, and will have good application prospects in future agricultural production.

The invention relates to a microbial flocculating agent. The microbial flocculating agent is prepared from grafted acrylamide and N,N'-methylene-bisacrylamide, wherein the microbial flocculating agentis produced by fermenting pseudomonas sp. DLXN-1 with the preservation number of CCTCC (China Center For Type Culture Collection) M20133305 and paenibacillus sp DLXN-2 with the preservation number ofCCTCC M20133306. The microbial flocculating agent provided by the invention has the properties of no toxicity, environment protection and safety; compared with an unmodified microbial flocculating agent, the crosslinking degree and complexity degree of a structure of the microbial flocculating agent are increased through grafting the microbial flocculating agent and a synthesized organic high polymer flocculating agent monomer; the purging and trapping capability is improved and the adsorption capability on metal plasmas is greatly increased through cationization modification, so that the flocculating capability of the grafted polymerized cation compound type microbial flocculating agent is greatly improved.

A high-temperature resistant floc-producing strain and its cultivation and application methods belong to the field of microbiology. Preserved by the General Microbiology Center (CGMCC) of the China Council for the Collection of Microbial Cultures, preservation number: CGMCC? No.2040, classification name: Paenibacillus? sp. Date of preservation: May 11, 2007. The high-temperature-resistant Paenibacillus strain screened from fruit tree soil is the first new strain discovered in China. After being heated at 40 to 80°C for 5 to 60 minutes, it is cultured through aerobic fermentation at 25 to 35°C. The fermentation liquid is used as a flocculant for kaolin suspensions. When the ratio is 0.01% to 0.2% (v / v), the flocculation rate is 88% to 97%. When the Paenibacillus and its fermentation liquid provided by the invention are used as kaolin suspension flocculant, the cultivation operation process is simple, the cost is low, the flocculation activity is high, the usage is low, and there is no secondary pollution.

The invention discloses a paenibacillus. The paenibacillus is classified as and named Paenibacillus sp., and is preserved on 6th July, 2016 in the common microorganism center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No.12738. The paenibacillus remarkably improves the growth states of populus and salix woody plants like increasing of root length, root number, height of seedlings and biomass, increases the survival rate, and is applied to the processes of growth and reproduction of the populus and salix woody plants.

The invention discloses a bacteriocin crude extract with bacteriostatic action, and a preparation method and application thereof. The preparation method of the bacteriocin crude extract comprises the following steps: (1) inoculating Paenibacillus sp. into a culture medium for culturing Paenibacillus sp., and carrying out shake fermentation and culture to obtain a fermentation liquid; and (2) centrifuging the fermentation liquid obtained in the step (1), taking the precipitate, adding acetone, evenly mixing, leaching, centrifuging, taking the supernatant, and removing acetone. The method solves the problem of narrow bacteriocin sources at present. Compared with the traditional bacteriocin, the bacteriocin crude extract prepared by the method has stabler properties, and thus, has higher food safety. The bacteriocin crude extract can be directly used for food production, thereby achieving the goals of inhibiting putrefying bacteria and prolonging the shelf life of food, and further lowering the application cost.

The invention provides paenibacillus (Paenibacillus sp.) WL-15 and application thereof in agarase preparation by microbial fermentation, wherein an agarase high-yield bacterial strain is obtained through ion implantation mutation. The bacterial strain is preserved in the China center for type culture collection, the preservation number of the bacterial strain is China center for type culture collection number: M2011357 (CCTCC No: M2011357), the preservation date of the bacterial strain is October 19th, 2011. The paenibacillus and the application of the paenibacillus have the advantages that 1a low energy N+ ion implantation method is adopted to perform mutation to produce agarase microorganisms, and has the advantages of being high in mutation rate, simple to operate and the like; 2 the agarase generated by the bacterial strain through mutation breeding is good in genetic stability, and a high-yield characteristic is not prone to degenerate; and 3 the bacterial strain has the advantages of being high in enzyme production activity, low in nutrition requirements, short in fermentation period and capable of being applied to agarase preparation by industrial fermentation. The agarase has various purposes in the field of agaro-oligo saccharide preparation and the like.

The invention belongs to the technical field of pesticides, and relates to a preparation method for a natural amicoumacin isocoumarin compound PM-94128 and an application of the compound. The compoundhas a chemical structure shown in a formula I in the description. The invention provides the method for preparing the compound from fermented products of Paenibacillus sp. strains and the applicationof the compound in crop fungicide preparation. Experimental research shows that the compound has different-degree antibacterial effects on rice pyricularia oryzae, maize puccinia polysra, and cucumber pseudoperonospora cubenis, and the application of the compound in crop fungicide preparation can be developed.