67 results about "Mycobacterium sp" patented technology

An isolated microorganism belonging to the genus Mycobacterium, having one or both of an inactivated gene Rv0757 that confers a PhoP− phenotype and an inactivated second gene which prevents the production of DIM (DIM− phenotype). Methods of making the microorganism, related pharmaceutical formulations and vaccines are also provided, as are related methods of treatment and vaccination. The pharmaceutical formulation may also serve as a vector or adjuvant.

Tuberculosis (TB) is a major health problem and currently, the only licensed TB vaccine is Mycobacterium bovis Bacille Calmette-Guerin (M. bovis BCG). In the present invention, mutation of mycobacterial components reportedly involved in phagosome maturation inhibition was evaluated for vaccine purposes, as such mutations should result in better vaccine antigen processing and presentation. Thus, BCG mutants in genes coding for ManLAM capping a-1,2-mannosyltransferases and the PI3P phosphatase SapM were evaluated as TB vaccines in a stringent mouse model. Vaccination with both ManLAM capping mutants and the SapM mutant resulted in significantly longer survival as compared to non-vaccinated mice, whereas vaccination with the parental BCG did not. Moreover, mice vaccinated with the SapM mutant survived significantly longer than mice vaccinated with the parental BCG.; The mutant BCG strains showed unaltered phagocytosis, replication, lysosome colocalization and oxidant activity in macrophages and similarly induced autophagy in the latter. Additionally, replication and granuloma formation in mice was unaffected, indicating BCG-equivalent safety of these vaccines.

Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genus Mycobacterium are disclosed. Kits including such oligonucleotides are disclosed. Methods of detecting Mycobacterium species using the oligonucleotides in in vitro nucleic acid amplification are disclosed.

The invention discloses high-efficiency organic pollutant carbendazim degrading bacteria and use thereof. The organic pollutant carbendazim degrading bacteria are (mycobacterium sp.)DJL22, and were collection in China General Microbiological Culture Collection Center, and the collection number of organic pollutant carbendazim degrading bacteria is CGMCC No.5041. When the (mycobacterium sp.)DJL22 CGMCC No.5041 is cultured in inorganic salt culture medium for 5 days, the rate of degradation of carbendazim at a concentration of 100mg / L reaches 84.79 percent; and when the (mycobacterium sp.)DJL22CGMCC No.5041 is cultured in inorganic salt culture medium for 15 days, the rate of degradation of carbendazim at a concentration of 600mg / L reaches 62.77 percent. Therefore, the stain can efficiently degrade carbendazim and has a bright application prospect in repairing soil polluted by carbendazim.

An object of the present invention is to provide an oligonucleotide for rapidly and conveniently detecting bacteria of the genus Mycobacterium (acid-fast bacteria) or for identifying the bacterial species thereof, and a method and kit for detecting bacteria of the genus Mycobacterium (acid-fast bacteria) using such oligonucleotid. The present invention provides a method for identifying Mycobacterium tuberculosis, which comprises performing a nucleic acid amplification reaction using a primer for nucleic acid amplification that comprises a nucleotide sequence corresponding to a variable region in a 16S rRNA gene sequence of Mycobacterium tuberculosis and has at least 3 continuous nucleotides contained in the nucleotide sequence represented by SEQ ID NO: 1 at the 3′ end.

The invention relates to a biochar-based immobilized microbial bacterial agent, a preparation method and application thereof. Biochar-based immobilized microbial bacterial agents include biochar-based materials, and bacterium and nutrients adsorbed on biochar-based materials. The bacterium is mycobacterium CSC-6 (Mycobacterium sp.CSC-6), in 2017 It was deposited in the China Center for Type Culture Collection on November 27, and its deposit number is: CCTCC No.M 2017726. The biochar-based immobilized microbial bacterial agent is used for degrading petroleum. Compared with the prior art, the bacterial agent prepared by the method of fixing and adsorbing bacterial agent + nutrient in the present invention has a higher degradation ability to petroleum hydrocarbons, and after 7 days of degrading sewage containing 1.0wt% petroleum, the degradation rate can reach 83.5% %, compared with the degradation rate of 52.0% of the simple bacterial agent, it has a greater improvement. At the same time, the biochar-based immobilized microbial agent is more conducive to the preservation of active bacteria.