Film strip for identifying mycobacterium tuberculosis, mycobacterium chelonae, mycobacterium abscessus, and preparation and use methods of film strip

Abstract

The present invention belongs to the field of microbial gene detection and particularly relates to a film strip for identifying mycobacterium tuberculosis, mycobacterium chelonae, mycobacterium abscessus, and preparation and use methods of the film strip. Specific probes comprise base sequences for detecting the mycobacterium tuberculosis in SEQ ID NO.1-2; base sequences for detecting the mycobacterium chelonae in SEQ ID NO.3-4; base sequences for detecting the mycobacterium abscessus in SEQ ID NO.5-6 and mycobacterium quality control probes SEQ ID NO.7-8; and corresponding primer pairs are SEQ ID NO.9-10. The established detection method can rapidly and accurately distinguish the mycobacterium tuberculosis, mycobacterium chelonae and mycobacterium abscessus, can be used as a useful supplementary identification method for a commercialized mycobacterium strain identification kit, and has good clinical application prospects.

Description

Technical field [0001] The invention belongs to the field of microbial gene detection, and specifically relates to a film strip for identifying Mycobacterium tuberculosis, Mycobacterium chelonae, and Mycobacterium abscesses, and a preparation and use method. Background technique [0002] Tuberculosis caused by Mycobacterium tuberculosis (M.TB) infection is still one of the most common infectious diseases in my country. Non-tuberculous mycobacterial disease refers to other branches except Mycobacterium tuberculosis and Mycobacterium leprosy Bacillus infection causes lesions of related tissues and organs. Berger’s Manual of Systematic Bacteriology divides nontuberculous mycobacteria (NTM) into fast-growing and slow-growing types according to their growth rate. Among the diseases caused by fast-growing NTM in my country, the most common type of bacteria is turtle / abscess. Mycobacterium. At present, there are already marketed gene chip kits for detecting Mycobacterium strains, which ...

AI Technical Summary

Problems solved by technology

At present, there are gene chip kits on the market for detecting mycobacterium strains, which adopt the principle of detecting 16SrRNA gene sequence, and the probes are fluorescently labeled, but they cannot distinguish between Mycobacterium chelonis and Mycobacterium abscessus, and comparison is required. It is completed by expensive laser confocal scanner, and the operation steps are more complicated

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