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Composite immobilized microbial agent as well as preparation method and application thereof

A technology of composite microbial inoculants and immobilized carriers, applied in biochemical equipment and methods, immobilized on or in inorganic carriers, and microorganism-based methods, etc. problems, to achieve a wide range of degradation substrates, improve the degradation rate, improve the effect of repair rate

Pending Publication Date: 2022-07-29
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When heavy metals and PAHs exist in the soil environment at the same time, there will usually be physical adsorption process, chemical process and biological process interaction between the two. Compared with the impact caused by single heavy metal pollution or PAHs pollution, this interaction Complicating the ecotoxic effects of heavy metals and PAHs
However, most of the existing research focuses on the pollution of a single category of heavy metals or PAHs in soil, and there are still few studies on the combined pollution control of heavy metals and PAHs.

Method used

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  • Composite immobilized microbial agent as well as preparation method and application thereof
  • Composite immobilized microbial agent as well as preparation method and application thereof
  • Composite immobilized microbial agent as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0066] The present embodiment provides a preparation method of an immobilized bacterial agent for repairing cadmium-polycyclic aromatic hydrocarbon composite polluted soil, comprising the following steps:

[0067] (1) Preparation of composite microbial bacterial solution

[0068] a. Preparation of Mucor sp. FMM suspension

[0069] Three colonies of Mucor sp.FMM were picked from the slant medium with an inoculating loop and cultured in PDA solid medium. After the 48h plate grows a colony of suitable size, pick a colony and inoculate it into the PDA liquid culture solution, under the conditions of 120r / min, 30℃, shake the culture for 24h, as the seed solution, inoculate the seed solution at 7500r / min. Centrifuge for 3 min, collect the bacterial cells, and resuspend the collected bacterial cells twice with PBS buffer to adjust the concentration of the bacterial solution to 1.0 g / L.

[0070] b. Preparation of Aspergillus niger SF05 suspension

[0071] Three colonies of Aspergillu...

Embodiment 2

[0080] In this example, conditions are changed on the basis of Example 1, and only step d in step (1) is changed to: Mucor sp. FMM suspension, Aspergillus niger SF05 suspension and Mycobacterium The sp.BFZG suspension was evenly mixed in a volume ratio of 2:1:1 to obtain a composite microbial bacterial solution.

Embodiment 3

[0082] In this example, conditions are changed on the basis of Example 1, and only step d in step (1) is changed to: Mucor sp. FMM suspension, Aspergillus niger SF05 suspension and Mycobacterium The suspension of Mycobacterium sp.BFZG was evenly mixed in a volume ratio of 1:1:3 to obtain a composite microbial bacterial solution.

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Abstract

The invention relates to a composite immobilized microbial agent which comprises a composite microbial agent and a composite immobilized carrier, the composite microbial agent comprises a Mucor sp.FMM microbial agent, an Aspergillus niger SF05 microbial agent and a Mycobacterium sp.BFZG microbial agent, and the composite immobilized carrier comprises a composite immobilized carrier and a composite immobilized carrier. The composite immobilized carrier comprises biochar and boric sludge. The composite immobilized microbial agent can be used for promoting remediation of cadmium-polycyclic aromatic hydrocarbon composite contaminated soil.

Description

technical field [0001] The invention relates to the technical field of environmental microbial restoration, in particular to a composite immobilized bacterial agent and a preparation method and application thereof. Background technique [0002] With the rapid economic development, the process of urbanization and industrialization has been accelerated, and the soil pollution near cities and industrial areas has become increasingly intensified. In addition, multiple pollutants in the soil coexist and interact with each other. The phenomenon of compound pollution has been particularly serious in recent years. The remediation of contaminated soil has become an important research direction in the field of environmental science. [0003] Compound pollution usually refers to the pollution formed by the coexistence of two or more pollutants of different types and properties in the same environment. Among them, heavy metals and polycyclic aromatic hydrocarbons (PAHs) are two types o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N1/14C12N1/20B09C1/10C12R1/785C12R1/685C12R1/32
CPCC12N11/14C12N1/14C12N1/20B09C1/10B09C1/105
Inventor 贾春云刘泽浩倪子钧常柳巩宗强李晓军代淑娟
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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