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Paenibacillus sp. strain HB172198 and applications thereof

A HB172198, Paenibacillus technology, applied in the field of microorganisms, can solve the problem of less Bacillus, and achieve the effect of mature technology

Active Publication Date: 2018-12-04
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus has the characteristics of strong adaptability, fast growth, and long survival time. Currently, few Bacillus producing alginate lyase have been reported.

Method used

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  • Paenibacillus sp. strain HB172198 and applications thereof
  • Paenibacillus sp. strain HB172198 and applications thereof
  • Paenibacillus sp. strain HB172198 and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Isolation and Screening of Paenibacillus HB172198 CGMCC No.15412

[0031] Brown algae samples were collected in the coastal waters of Qishui Bay, Wenchang City, Hainan Province. Take 10g sample, cut it into pieces under aseptic condition, dissolve the slurry, put it in a 250mL Erlenmeyer flask filled with 90mL sterile water and glass beads, shake it in a constant temperature shaker at 28°C and 200r / min for 30min, and let it stand After 5 minutes, the supernatant was taken for 10-fold serial dilution, and diluted to 10 -1 ~10 -3 series of suspensions. Use 80°C water bath for 20 minutes to kill non-spore-forming bacteria, draw 0.1mL of the series suspension, spread it on the alginate lyase separation medium, place it in a 30°C incubator and incubate it upside down for 2-5 days.

[0032] When the colonies grow out, according to the phenotypic characteristics of the colony shape, color, edge state, transparency, surface dry and wet state, etc., pick the colonie...

Embodiment 2

[0040] Example 2 Identification of the species of Paenibacillus HB172198 CGMCC No.15412

[0041] Strain HB172198 CGMCC No.15412 was streak cultured on 2216E agar medium for 3 days, the colony was round, with regular edges, matte, flat, light yellow, opaque; the bacteria were rod-shaped, spore-forming, with flagella at both ends ( figure 1 ). Gram staining is variable, nitrates are not reduced, gelatin is not hydrolyzed, and indoles are not produced.

[0042]The 16S rDNA sequence 1477bp of HB172198 was obtained by PCR amplification and sequencing, and its nucleotide sequence is shown in SEQ ID No.1. Comparing the sequence with the sequence in the EzBioCloud database, it was found that the strain HB172198 and Paenibacillus lemnae L7-75 T The homology is the highest (97.6%), and the homology with other strains is all below 97.0%. Select related strains with high homology, and use the software MEGA5.0 to construct a phylogenetic tree using the Neighbor-joining method ( figur...

Embodiment 3

[0046] Embodiment 3 alginate lyase and preparation method thereof

[0047] The alginate lyase in the present invention is produced by fermentation of Paenibacillus HB172198 with the preservation number CGMCC No.15412 in Example 1, and the specific method includes the following steps:

[0048] (1) Bacterial strain activation: Inoculate the Paenibacillus HB172198 in Example 1 on a solid medium, and culture it at 30° C. for 48 hours to obtain an activated bacterial strain;

[0049] (2) Liquid culture: insert the activated bacterial strain into the liquid seed medium, shake and culture at 30°C and 180r / min for 12-24 hours, and make the seed liquid;

[0050] (3) Fermentation culture: Inoculate the seed liquid at 3% by volume in the liquid fermentation medium, culture at 30°C and 180r / min for 36 hours with shaking, collect the fermentation liquid, centrifuge, and collect the supernatant to obtain alginate cracking Enzyme Crude Enzyme Solution.

[0051] The solid medium in step (1)...

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Abstract

The invention discloses a paenibacillus sp. strain HB172198; the systematic name of the strain HB172198 is Paenibacillus sp.; the preservation number is CGMCC No.15412; the preservation date is 5th March, 2018; the preservation institution is China General Microbiological Culture Collection Center, CGMCC; and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3building No.1 yard Beichengxi road, Chaoyang district, Beijing. The invention also discloses an alginate lyase and a preparation method thereof, and an application of the paenibacillus sp. strain andthe alginate lyase in degradation of brown algae.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Paenibacillus strain HB172198 and an application thereof. Background technique [0002] Alginate, also known as sodium alginate, is composed of 1,4-β-D-mannuronic acid (1,4-β-D-mannuronic acid, M) and 1,4-α-L-guluronic acid Acid (1,4-α-L-guluronic acid, G) two uronic acid monomers are linked by α / β-1,4 glycosidic bonds and randomly arranged into linear polymers, which widely exist in kelp, sargassum, In the cells of brown algae such as macroalgae and carrageenan. As a polysaccharide with great application value, alginate can be degraded into oligosaccharide fragments composed of 2 to 20 monosaccharides, thus exhibiting a variety of biological activities. It is widely used in food, medical and industrial production, etc. Wide range of industry applications. Alginate degradation methods are divided into chemical degradation, physical degradation and enzymati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/88C12P19/04C12P19/12C12P19/00C12R1/01
CPCC12N1/20C12N9/88C12P19/00C12P19/04C12P19/12C12N1/205C12R2001/01
Inventor 鲍时翔黄惠琴郑志国朱军邹潇潇胡永华刘敏
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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