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Microbial flocculating agent generated by paenibacillus and applications thereof

A microbial flocculant and Paenibacillus technology, applied in the field of microbial flocculants, can solve the problems of restricting the production and application of microbial flocculants, large dosage of polyaluminum chloride, and high cost, so as to improve the flocculation effect and settling speed quickly. , the effect of flocs

Active Publication Date: 2017-05-10
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbial flocculants have the advantages of non-toxic, safe and efficient, and biodegradable, but as described in Document 1 (Gan Li, Meng Zhaoping, Research Progress of Microbial Flocculants, Water Treatment Technology, 2006, 32 (4): 5-9) , problems such as large dosage and high cost seriously restrict the production and application of microbial flocculants
At present, the compound use of microbial flocculants and other flocculants is a direction of microbial flocculant research. Many studies have shown that the compound use of microbial flocculants can not only improve the flocculation effect, but also greatly reduce the use of flocculants Dosage, such as literature 2 (Huang Jing, Yang Zhaohui, Sun Peishi, response surface optimization of microbial flocculant and polyaluminum chloride compound, Chinese Environmental Science, 2008, 28 (11): 1014-1019) will microbial flocculant and chlorination Calcium and polyaluminum chloride are used in combination, and the flocculation effect is greatly improved, but the amount of polyaluminum chloride used is still relatively large

Method used

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  • Microbial flocculating agent generated by paenibacillus and applications thereof
  • Microbial flocculating agent generated by paenibacillus and applications thereof
  • Microbial flocculating agent generated by paenibacillus and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Screening, isolation and identification of Paenibacillus sp.LX03.

[0027] (1) Screening and isolation of strains

[0028] Soil samples collected from Yancheng City, Jiangsu Province were dissolved in distilled water, and after 1 hour, the supernatant was diluted with 10 2 ~10 6 times, prepare Htm solid medium, spread 100 μL of the previously diluted samples respectively, place them in a biochemical incubator, and incubate at 30°C for 2-3 days. Select the strains with obvious differences in the characteristics of the colonies on the petri dish, and use the dilution coating method to purify until a single strain is obtained, and its colony morphology is as follows: figure 1 shown.

[0029] The composition of Htm liquid medium is as follows: starch 20g / L, sodium dihydrogen phosphate 1g / L, potassium nitrate 3g / L, calcium chloride 0.07g / L, magnesium chloride 0.2g / L, ferrous sulfate heptahydrate 0.0125g / L , manganese sulfate 0.003g / L, zinc chloride 0.0075g / L, the solvent...

Embodiment 2

[0033] Preparation process of microbial flocculant produced by Paenibacillus sp.LX03 strain

[0034] Paenibacillus sp.LX03 was inoculated into the fermentation medium, the culture temperature was 30°C, and the shaker was cultivated at 250rpm for 2 to 3 days to obtain the fermentation broth containing the microbial flocculant. After removing the bacteria by centrifugation at 7000rpm for 10min, Add 2 to 3 times the volume of ethanol to precipitate extracellular products, and then add distilled water to dissolve the extracellular products after drying to obtain the microbial flocculant. The fermentation medium is the above-mentioned Htm liquid medium.

Embodiment 3

[0036] Study on the flocculation performance of microbial flocculant produced by Paenibacillus sp.LX03 strain.

[0037] Prepare kaolin suspension (4g / L) as simulated wastewater to test the flocculation performance of microbial flocculants. The specific method is: put 50mL of kaolin suspension (4g / L) in a 50mL beaker, add a certain amount of microbial flocculant, stir with a magnetic force at 100rpm at room temperature for 1min, and after standing still for 5min, take a liquid sample at the middle height and measure it in the spectrophotometer. Measure its absorbance at a wavelength of 550nm, and use distilled water instead of microbial flocculant as a control group.

[0038] The formula for calculating the flocculation rate is:

[0039] Flocculation rate=(A﹣B) / A×100%

[0040] Among them, A is the absorbance of the control group at 550nm, and B is the absorbance of the experimental group at 550nm.

[0041] (1) Flocculation characteristics of microbial flocculants

[0042] C...

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Abstract

The invention discloses a microbial flocculating agent generated by paenibacillus and applications thereof. The microbial flocculating agent is generated by paenibacillus sp. LX03, and is prepared by the following steps: inoculating paenibacillus sp. LX03 into a fermentation culture medium, carrying out cultivation on a shaking table for 2 to 3 days, subjecting the fermentation broth to centrifugation to remove bacterial cells, adding ethanol, which is 2 to 3 times the volume of the fermentation broth, to precipitate extracellular products, drying the extracellular products, and adding water to dissolve extracellular products to obtain the microbial flocculating agent. When the microbial flocculating agent is compounded with iron ions and aluminum ions, a good flocculating effect can be achieved. When the microbial flocculating agent is used with an inorganic flocculating agent namely polymeric aluminum chloride, the using amount of polymeric aluminum chloride is largely reduced, and a better flocculating effect is formed in a short time. The microbial flocculating agent can be used as a good water treatment flocculating agent and flocculating aid, and can be widely used in the fields such as household wastewater treatment, industrial wastewater treatment, and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial flocculant water treatment, and in particular relates to a microbial flocculant produced by Paenibacillus and its application. Background technique [0002] The massive discharge of domestic sewage and industrial sewage has seriously polluted the environment and affected the ecological balance. The flocculant can combine with the pigments, solid suspended particles, heavy metal ions and other pollutants in the sewage to form larger flocs and then settle, which is convenient for the centralized treatment of various pollutants. After the flocculant treatment, the water quality of the sewage get cleansed. At present, flocculants can be roughly divided into three categories: inorganic flocculants, including iron salts, aluminum salts, polyaluminum chloride, polyferric chloride, etc.; synthetic organic polymer flocculants, such as polyacrylamide and its derivatives; Natural biological flocculants, ...

Claims

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Application Information

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IPC IPC(8): C02F1/52C02F1/54C12P1/04C12R1/01C02F101/20
CPCC02F1/52C02F1/5236C02F1/5245C02F1/54C02F2101/20C12P1/04
Inventor 翁丹林洪发张建法
Owner NANJING UNIV OF SCI & TECH
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