The present invention discloses a yogurt jam pudding containing active probiotics and a preparation method thereof. The yogurt jam pudding comprises the following components in parts by weight: 28-32parts of white granulated sugar, 90-110 parts of high fructose corn syrup, 25-35 parts of glucose monohydrate, 10-15 parts of pudding powder, 20-28 parts of whole milk powder, 15-20 parts of skim milkpowder, 1.8-2.5 parts of essences, 2.0-2.5 parts of citric acid, 1.4-1.8 parts of malic acid, 4.2-4.6 parts of potassium sorbate, 2.0-3.0 parts of titanium dioxide, 660-700 parts of softened water, 18-22 parts of a lactic acid bacterium fermented raw solution and 75-85 parts of a blueberry, strawberry and cranberry jam. The yogurt jam pudding is simplified in processing flows during the preparation. The milk and other materials in an early stage are sterilized, the sterilized materials are cooled, the cooled materials are inoculated with bacteria, then the sterile jam is added, then the materials are subjected to a sterile filling method, the pudding product contains the probiotics, and the preparation method extends shelf life of the product, enables the shelf life of the yogurt jam pudding to be extended to 45 days, and can realize industrial production.