34results about How to "Retain vitality" patented technology

The invention discloses an F175-3 protein, a coding gene thereof and an application of the F175-3 protein to hydrolyzing cellulose. The invention provides the protein shown in the following 1) or 2): 1) a protein formed by amino acid residues shown in a sequence 2 in a sequence table; 2) a protein which is obtained by carrying out substitution and / or deletion and / or addition of one or more amino acid residues on an amino acid residue sequence of the sequence 2 in the sequence table, has the same functions as the protein shown in 1) and is derived from the protein shown in 1). Experiments prove that new cellulase F175-3 can still retain 88.5% of activity after being incubated at an optimum temperature of 60 DEG C for two hours under the conditions that cellulose is taken as the substrate and the pH value is 5.0; the new cellulase F175-3 can retain more than 80% of residual activity in a pH value range of 3.5-8.0 after being incubated in buffer solutions with different pH values for 24 hours at 4 DEG C.

The invention discloses a preparation method of duck reovirus yolk antibody, which comprises the following steps: (1) using duck reovirus inactivated vaccine to immunize breeding-aged hens to obtain high-immunity eggs; (2) collecting high-immunity eggs Egg yolk, after removing the vitelline membrane and frenulum, crush it with a colloid mill to obtain egg yolk liquid; (3) mix the egg yolk liquid obtained in step (2) with purified water at a volume ratio of 1:1 to 3, stir well and then add The final concentration weight volume ratio is 1-3% citric acid and 1-4% polyanion cellulose, after fully stirring, place it at low temperature for 3-6 hours, centrifuge at 1-5 ° C, and collect the supernatant; (4) the step (3) The collected supernatant was sterilized by filtration to obtain egg yolk antibody liquid. The preparation method of duck reovirus yolk antibody disclosed by the invention has simple steps, high efficiency and yield, less addition, and is economical and applicable.

The invention discloses a process for separating albumin from hemolyzed serum, which is characterized in that it comprises the following steps: (1) adding activated carbon to the hemolyzed serum, freezing, and then standing at normal temperature until the serum is completely frozen-thawed and stratified, Extract the upper layer solution, filter and collect filtrate A; (2) Gel chromatography the filtrate A, collect the eluent to obtain solution B; (3) add carboxylated ferric oxide nano-magnetic beads to solution B, and after incubation at room temperature, Filtration and separation to obtain solution C; (4) ultrafiltration and concentration of solution C to obtain protein concentrate D; (5) virus inactivation and drying of protein concentrate D to obtain albumin powder. The raw material cost of the method is low, and the prepared albumin has good purity and high safety.

The invention discloses a durian yogurt pudding and a preparation method thereof. The composition per 100 parts by weight includes: 60-80 parts of milk, 1-5 parts of whole milk powder, 0.1-2 parts of durian pulp, and 1-6 parts of cream 0.5-1.2 parts of sweetener, 0.5-1.2 parts of compound emulsifying thickener, 0.05-0.15 parts of essence, 0.02-0.03 parts of mixed bacteria powder of Streptococcus thermophilus and Lactobacillus bulgaricus, and the balance of water. The durian yogurt pudding has the advantages of high nutritional value, fine and smooth taste, uniform and stable, and long shelf life. The preparation method simplifies the technological process, is suitable for industrial production, and ensures the vitality of bacteria to the greatest extent, enhances the nutritional and functional characteristics of the product, and is more beneficial to the health of the human intestinal tract.

The invention discloses a cartilage tissue low-temperature refrigeration preservation solution and application thereof. The preservation solution comprises one or more of a DMEM medium, 1-30mM / L HEPES, a 1-20mM / mL component A, a 10-100ng / mL transforming growth factor beta, a 10-100ng / mL component B, 10-100g / L chondroitin sulfate and a 100-1000microg / mL matrix metalloproteinase inhibitor, wherein the component A is bongkrekic acid or Q-VD-OPH; and the component B is cartilage-derived morphogenetic protein 1, BMP or PDGF. The preservation solution provided by the invention can prolong the preservation time of fresh cartilage, and after preservation for 8 weeks, the cartilage still meets the requirements of clinical transplantation for activity and functionality.

The invention discloses a controllable culture method of cell density and arrangement based on an insect wing substrate. The method uses insect scale wings as a base, and the insect scale wings are placed obliquely in a cell suspension, and the cells fall vertically under the action of gravity. On the surface of the insect scales, the number of cells gradually increases from top to bottom; the cells on the surface of the insect scales are oriented according to the micro-nano structure on the surface of the insect scales. The invention uses insect scales as the substrate, which is easy to obtain, safe and non-toxic, and can realize cell culture with controllable cell density and arrangement at the same time, and avoids a series of complex processes and safety problems caused by artificial synthetic materials; it uses gravity to form The density gradient distribution avoids the damage of mechanical force to cells and retains cell viability. The formed cell arrangement with directional arrangement and density gradient distribution has potential application prospects and reality for tissue regeneration, including regeneration and repair of connective tissue. significance.

The invention discloses an ice crystal-free cryopreservation solution and a freezing method for cartilage, tendon and meniscus preservation. 0.1-2.5mol / L of piperazine ethylsulfonic acid, Caspase inhibitor Z-VAD-FMK 10-50μmol / L, matrix metalloproteinase inhibitor GM6001 and component X of 2-10mg / L. The preservation solution disclosed by the present invention can effectively increase the number of living cells and cell viability in cartilage, tendon, and meniscus tissue after cryopreservation, and more importantly, it can prevent ice crystals from growing again during freezing recovery and during storage. vitrified. This new type of ice crystal-free cryoprotectant allows cartilage, tendon, and meniscus to be stored for a longer period of time, and can be stored permanently at an environment of less than ‑130 °C.

The invention discloses a preparation method of a directed vat set chymosin preparation and a product and an application thereof. The preparation method comprises the following steps: culturing paenibacillus (Paenibacillus sp.) at 25-35 DEG C by using a skimmed milk culture medium, shake culturing for 24-120 hours to obtain fermentation broth, centrifuging the fermentation broth and extracting the supernatant to obtain the directed vat set chymosin preparation. According to the preparation method, the skimmed milk is innovatively used as a base material for fermentation, since being a milk product as well, the obtained chymosin can be directly applied to the preparation process of cheese, compared with the traditional chymosin preparation method, the method is used for greatly simplifying the application steps of the chymosin preparation, retaining the chymosin activity to the uttermost and completely avoiding the enzyme activity loss of chymosin in the extraction process, so as to effectively reduce the production and application costs of the chymosin preparation.

The invention discloses F52-2 protein and application of a coding gene of the F52-2 protein and hydrolyzed xylan. The protein disclosed by the invention is protein (1) or (2), wherein the protein (1) is formed by amino acid residues shown in a sequence 2 in a sequence table, and the protein (2) is formed through enabling a residue sequence of an amino acid sequence of the sequence 2 in the sequence table to be subjected to the substitution and / or deletion and / or addition of one or more amino acid residues and is protein which has the same functions and is derived from the protein (1). According to the F52-2 protein and the application of the coding gene of the F52-2 protein and the hydrolyzed xylan, proved by experiments, the optimal temperature for novel beta-xylosidase F52-2 is 60 DEG C under the conditions of taking p-nitrophenol xyloside (pNPX) as a substrate and being in the pH value of 5.0, and activity of 85% can still be maintained after the novel beta-xylosidase F52-2 is incubated for 2 hours at the temperature of 50 DEG C; over 60% residual activity can be maintained in the pH value of 3.5-5.5 after the novel beta-xylosidase F52-2 is incubated for 24 hours in buffer solutions with different pH values at the temperature of 4 DEG C.

The invention discloses a preparation method of a pastevula mulfocida capsular antigen A group yolk antibody. The method includes the following steps that (1) a pastevula mulfocida capsular antigen Agroup inactivated vaccine is used for immunizing reproductive-age hens to obtain high-immune eggs; (2) high-immune egg yolks are collected, yolk membranes are removed, and crushing is carried out to obtain yolk liquid; (3) the yolk liquid obtained in step (2) and purified water are mixed in a volume ratio of 1:(2-4) and stirred to be uniform, L-ascorbic acid with a final concentration of 1-3% (m / v), propylene glycol with a final concentration of 4-8% (m / v) and dextran sulfate with a final concentration of 3-6% (m / v) are added, after sufficient stirring, the mixture is placed for 2-4 h at a lowtemperature, low-temperature centrifugation is carried out, and supernate is collected; (4) the supernate collected in step (3) is subjected to filtration sterilization to obtain yolk antibody liquid. The preparation method of the pastevula mulfocida capsular antigen A group yolk antibody includes simple steps, has high efficiency and yield and is economical and applicable.