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A kind of cartilage tissue cryopreservation solution and its application

A technology of cartilage tissue and preservation solution, which is applied in the field of biomedical engineering, can solve the problems of low survival rate of chondrocytes, achieve the effects of improving survival and functional recovery, retaining activity, and good tissue functionality

Active Publication Date: 2020-07-28
江苏科瑞百奥生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] (2) Long-term preservation of chondrocyte survival rate is still low

Method used

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  • A kind of cartilage tissue cryopreservation solution and its application
  • A kind of cartilage tissue cryopreservation solution and its application
  • A kind of cartilage tissue cryopreservation solution and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Take 500mL DMEM cell culture medium and take a sample in a biological safety cabinet for sterility testing.

[0047] 2. Add chondroitin sulfate to be configured as a DMEM cell culture medium with a chondroitin sulfate content of 75g / L.

[0048] 3. Add HEPES to the previous step to prepare a homogeneous cartilage preservation solution with a HEPES content of 20mmol / L.

[0049] 4. Add rice yeast acid, transforming growth factor β, and cartilage-derived morphogenetic protein 1, and prepare rice yeast acid 10mmol / mL, transforming growth factor β50ng / mL, cartilage-derived morphogenetic protein 1 10ng / mL, pH7 .30-7.50.

[0050] 5. Put the solution in the previous step into a sterile container, seal it, and store it in a low-temperature environment at 4°C away from light.

Embodiment 2

[0052] 1. Take 500mL DMEM cell culture medium and take a sample in a biological safety cabinet for sterility testing.

[0053] 2. Add chondroitin sulfate to be configured as a DMEM cell culture medium with a chondroitin sulfate content of 75g / L.

[0054] 4. Take 500mL of DMEM cell culture medium that has passed the sterility test, and add 200mg of GM6001 to it to prepare a DMEM cell culture medium with a GM6001 content of 400ug / mL.

[0055] 5. Add HEPES to the previous step to prepare a homogeneous cartilage preservation solution with a HEPES content of 20mmol / L.

[0056] 6. Add rice yeast acid, transforming growth factor β, and cartilage-derived morphogenetic protein 1, and prepare rice yeast acid 5mmol / mL, transforming growth factor β10ng / mL, cartilage-derived morphogenetic protein 1 50ng / mL, pH7 .30-7.50.

[0057] 7. Put the solution in the previous step into a sterile container, seal it, and store it in a low-temperature environment at 4°C away from light.

Embodiment 3

[0058] Embodiment 3 (implementation cases with large differences):

[0059] 1. Take 500mL DMEM cell culture medium and take a sample in a biological safety cabinet for sterility testing.

[0060] 2. Add chondroitin sulfate to be configured as a DMEM cell culture medium with a chondroitin sulfate content of 100 g / L.

[0061] 4. Take 500mL of DMEM cell culture medium that has passed the sterility test, and add 400mg of GM6001 to it to make a DMEM cell culture medium with a GM6001 content of 800ug / mL.

[0062] 3. Add HEPES to the previous step to prepare a homogeneous cartilage preservation solution with a HEPES content of 10mmol / L.

[0063] 4. Add rice yeast acid, transforming growth factor β, and cartilage-derived morphogenetic protein 1, and prepare rice yeast acid 20mmol / mL, transforming growth factor β100ng / mL, cartilage-derived morphogenetic protein 1 100ng / mL, pH7 .30-7.50.

[0064] 5. Put the solution in the previous step into a sterile container, seal it, and store it...

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Abstract

The invention discloses a cartilage tissue low-temperature refrigeration preservation solution and application thereof. The preservation solution comprises one or more of a DMEM medium, 1-30mM / L HEPES, a 1-20mM / mL component A, a 10-100ng / mL transforming growth factor beta, a 10-100ng / mL component B, 10-100g / L chondroitin sulfate and a 100-1000microg / mL matrix metalloproteinase inhibitor, wherein the component A is bongkrekic acid or Q-VD-OPH; and the component B is cartilage-derived morphogenetic protein 1, BMP or PDGF. The preservation solution provided by the invention can prolong the preservation time of fresh cartilage, and after preservation for 8 weeks, the cartilage still meets the requirements of clinical transplantation for activity and functionality.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a cartilage tissue cryopreservation solution and its application. Background technique [0002] Articular cartilage is an elastic load-bearing tissue that makes up the movable articular surface. It has the characteristics of reducing friction, absorbing shocks, conduction, and buffering loads. It provides the above biomechanical functions for the human body for decades. Up to now, there is no material that can replace it. Function of articular cartilage. [0003] When the articular cartilage is severely damaged or diseased, the replacement transplantation of articular cartilage containing active chondrocytes is an ideal treatment method (the more common ones are meniscus and ankle articular cartilage transplantation). However, in the current clinical treatment process, the common treatment methods are mainly drug relief and artificial joint (metal composite mate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/021
Inventor 宋云庆欧阳溪
Owner 江苏科瑞百奥生物技术有限公司
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