The present invention relates to the technology field of the
biomedicine, in particular to a preparation method for the rapid dissolving of a
blood culture medium. Firstly, the raw materials of the culture medium are weighed, and the raw materials include that peptone is 10 g,
beef extract is 3 g,
sodium chloride is 5 g,
sodium citrate is 3 g, and
agar is 0.5 g; the fast solution is prepared by the preparation method that 24.6 percent of
magnesium sulfate solution is prepared to be used as fast solution 1 to be reserved; 0.5 percent of paraaminobenzoic acid solution is prepared to be used as fast solution 2 to be reserved; the mixing is realized by that about 20 ml
raw material of the fast solution is taken and 10 ml fast solution is taken to be added into the
raw material of the culture medium and agitated into paste, and then 1000ml
distilled water is added into to be rocked and mixed, and statically positioned for three to five minutes to obtain the transparent culture medium fluid; the pH value is adjusted to be 7.4; the culture medium fluid is subpackaged in a sealed liquid
bottle, and then sterilized in the
high pressure; the finished product of the
blood culture medium includes the obtaining process that 100 units of penicillase are added in each
bottle through an injection
syringe or not added, after the
sterility test, the
blood culture medium is reserved in the
room temperature. Compared with the prior art, the present invention eliminates the long time heating dissolving and filter process, saves the preparation time and the
filter material, and the operation issimple and convenient.