69 results about "Sterility test" patented technology

An apparatus, system, and method are disclosed for automatically testing a plurality of software test cases. The testing executes a quick test of the test cases which executes each test case in a test environment that is initialized just prior to the first test case and after subsequent test case failures. The testing further executes an adjusted test of the failing test cases in which delay parameters associated with the failing test cases are increased in accordance with a system load recorded during the quick test. Finally, the testing executes a sterilized test of the remaining failing test cases in a test environment that is initialized prior to each test case execution.

A sterility test method includes: selecting strain and culture medium, preparing bacterial cultures, transcribing fingerprint characteristics in thermograms as indices to verify the characteristics, drawing the thermodynamic parameters of the thermogram, determining the positive judgment index and performing sterility test for the samples. A fully-enclosed bacteria collecting ampoule incubator includes bacteria collecting ampoule system, sample and liquid feeding system and peristalsis liquid discharge system. The sample and liquid feeding system is connected with the bacteria collecting ampoule system by the liquid intake tube; and the bacteria collecting ampoule system is connected with the peristalsis liquid discharge system by the liquid drainage tube. The invention is characterized by short inspection time, high sensitivity, high automation and accurate test results on microbial contamination. It can also provide the overall process curve on the growth conditions. Such curve is provided with relatively favorable fingerprint, which enables qualitative analysis on the microbial contamination conditions.

The invention discloses a separation and selection method of antibiotic antitubercular endophyte, which comprises the steps as follows, the preparation of antibiotic antitubercular plant tissue, the surface sterilization and the sterility test, the separation and the purification of the endophyte, the ferment of endogenetic fungi, the ferment of endogenetic bacterium, the process of the zymotic fluid, the process of the thalli, the selection of the effective antibiotic bacterial strains, the selection of the effective antitubercular bacterial strains and the preservation of the strains. The separation and selection method of antibiotic antitubercular endophyte ensures the realization of obtaining the effective antibiotic antitubercular matter from microorganisms, thanks to separation and selection method, a batch of effective bacterial strains are obtained.

The invention discloses a method for storing endometrial stem cells, which comprises the following steps: 1) preparing a collection tool set and preparing a culture medium; 2) collecting menses; 3) performing a sterility test; and 4) subjecting endometrial stem cell to separation culture, namely, filtering mixed liquid, which is obtained by the step 2), in a collection tube 1, collecting single karyoplasts by using a density gradient centrifugation method, inoculating the obtained single karyoplasts in a Chang complete culture medium at an inoculation density of single karyoplasts of 1*10<5> to 1*10<6>/ml, and culturing in a culture tank which is at 37 DEG C and saturated humidity and contains CO2 at a volume percentage concentration of 5 percent; 5) amplifying and purifying cells; and 6) storing cells by freezing. When the method of the invention is used, a large number of high-purity target cells, namely endometrial stem cells, can be obtained.

The invention discloses a gynecological medical gel dressing and a preparation method thereof, and aims at providing a gynecological medical gel dressing being remarkable in effect of treating various gynecologic inflammations and capable of quickly repairing disseminated congestion, mucosal injury and superficial ulcer caused by vagina inflammatory diseases and cervical erosion. According to the technical points, the dressing comprises chitosan at a concentration of 1.25 to 2.0% and other auxiliary materials; and the preparation method comprises the following steps of: weighing chitosan and dissolving by 0.5% of lactic acid solution to obtain chitosan liquid; slowly adding carbomer accounting for 1% of the total mass and glycerinum accounting for 10% of the total mass to the chitosan liquid; uniformly stirring; adding chlorhexidine acetate accounting for 0.1% of the total mass; uniformly mixing; adjusting pH (Potential of Hydrogen) to 4.5 to 7.5 through a sodium bicarbonate solution; filtering; removing bubble through vacuum equipment to obtain a semi-finished product; detecting biological indexes and physicochemical indexes the next day; performing split charging through a hose syringe; carrying out sterility test, thus obtaining the finished product after confirmation. The gynecological medical gel dressing and the preparation method thereof belong to the technical field of pharmaceutical preparation.

The invention discloses a stable ifenprodil tartrate injection which contains ifenprodil tartrate, an osmotic pressure regulator, a pH regulator and water for injection. The invention also discloses a preparation method of the stable injection. The stable ifenprodil tartrate injection is obtained by controlling the range of the pH value of the solution and insulating oxygen, thus avoiding use of an antioxidant and possible adverse reactions thereof. Sterility tests, accelerated tests and long duration tests prove the stable ifenprodil tartrate injection.

The invention relates to a fish vaccine and a preparation method thereof, in particular to an aeromonas hydrophila inactivated vaccine and the preparation thereof. The aeromonas hydrophila inactivatedvaccine is prepared by the following concrete steps: separating out a pathogen from a sick fish, determining the pathogen as an aeromonas hydrophila through morphology, virulence factors, biochemicalreaction and gene sequencing; rejuvenating the strain, selecting an optimum condition for cultivation; then selecting the optimum condition for inactivation to prepare the aeromonas hydrophila inactivated vaccine, and conforming the aeromonas hydrophila inactivated vaccine to be certificated through sterility test and security test. The inactivated vaccine is prepared from a pathogenic bacterialstrain separated from a sick fish body, has a simple operating method, strong pertinency, strong protective function, good immunological effect and low cost of the used material, and is suitable for mass production. The invention provides a practical method for preventing and curing the bacteremic septicemia of carps.

The invention discloses a double-sided sterility test process isolator. The isolator is characterized by comprising a glove bin system and an electrical cabinet system, wherein an air treatment system is arranged at the top of the glove bin system; the glove bin system comprises a bin body; a glove operation component is arranged on the bin body, and comprises operation window sealing glass; and gloves are connected with the operation window sealing glass by virtue of a glove connection component. The sterility test process of sterile medicines can be carried out under a controlled environment all the time by virtue of the sterility test isolator, so that toxic substances inside the sterile medicines cannot hurt operators, pollutants in the external environment are prevented from entering the operation space and polluting to-be-tested products, and the requirement of a production region for the cleanness of a background environment can be reduced. Changes of an arbitrary environment parameter inside the sterility test process isolator can be correspondingly regulated and controlled, so that isolation of the sterility test step after production of sterility medicines can be realized.

A system that indicates the presence or absence of microorganisms in fluid food products. The system has a bottle for receiving sample to be tested. The bottle has a sensor that will monitor and detect changes in at least one sample parameter, but no additives that contain nutrients that support microbial growth. The bottle is placed in an incubator and the sensor in the bottle is monitored for changes. The incubator is programed so that, if the sensor detects that the value of the monitored parameter has reached a certain value, then the sample is determined to be positive for microbial growth.

The present invention discloses a preparation method for a microencapsulated oral live vaccine of gosling plague. The preparation method comprises: preparing a gosling plague SYG strain into a vaccine half finished product by goose embryo proliferation culture; adding a 5% sucrose and skimmed milk solution after a sterility test is qualified, and uniformly stirring; adding 20-40% porous starch tothe half finished product solution, stirring for 20-40 minutes at a certain temperature, wherein the temperature is controlled to 37 DEG C; mixing the resulting liquid and a 1-2.5% sodium alginate solution, completely stirring, and then carrying out dehydration and drying by a fluidized bed to prepare the microencapsulated vaccine dry powder of the gosling plague, wherein the volume of the resulting liquid is the same as the volume of the sodium alginate solution. The method of the present invention ha characteristics of science, simpleness, stable and reliable production, less loss of vaccine titer. With adopting the vaccine microencapsulation technology, the live vaccine of the gosling plague can be adopted for immunization by the oral route, the stress reaction is reduced, the sustained release function is provided for the live vaccine of the present invention, and the breeder goose in the laying period can use the live vaccine as usual.

The invention relates to a human sperm cryoprotectant containing D-trehalose and a preparation method of the human sperm cryoprotectant containing the D-trehalose and mainly solves the technical problems of low thawing rate and sperm motility decline in use of existing human sperm slow cryoprotectants.The technical scheme includes that the every 100ml of the human sperm cryoprotectant comprises 1.5-1.6g of glucose, 1.3g of trisodium citrate dehydrate, 1.3g of glycine, 20ml of glycerin, 20ml of egg yolk, 2.0-2.5g of the D-trehalose and 60ml of deionized water.The preparation method includes: 1) adding the glucose, the trisodium citrate dehydrate and the D-trehalose to obtain a mixed solution; 2) adding the glycerin and the glycine; 3) using a filter for filtering; 4) adding the egg yolk; 5) incubating in a water bath; 6) detecting a pH value of the solution; 7) performing sterility test; 8) performing sperm survival test; 9) storing in a test tube at -70 DEG C.

Device and method for sterility testing, in particular of pharmaceutical products, includes a closed sterility test system having at least two test containers, in each case having an integrated test filter, an outlet connecting branch arranged below the test filter and an inlet connecting branch which is arranged above the test filter and can be connected with a tube connection via a sampling device to a sample container. The tube connection of the test container in each case has a distributing component via which a fixed tube connection to an associated nutrient medium container is formed and can be used to feed an associated nutrient medium to the test container.

The present invention relates to the technology field of the biomedicine, in particular to a preparation method for the rapid dissolving of a blood culture medium. Firstly, the raw materials of the culture medium are weighed, and the raw materials include that peptone is 10 g, beef extract is 3 g, sodium chloride is 5 g, sodium citrate is 3 g, and agar is 0.5 g; the fast solution is prepared by the preparation method that 24.6 percent of magnesium sulfate solution is prepared to be used as fast solution 1 to be reserved; 0.5 percent of paraaminobenzoic acid solution is prepared to be used as fast solution 2 to be reserved; the mixing is realized by that about 20 ml raw material of the fast solution is taken and 10 ml fast solution is taken to be added into the raw material of the culture medium and agitated into paste, and then 1000ml distilled water is added into to be rocked and mixed, and statically positioned for three to five minutes to obtain the transparent culture medium fluid; the pH value is adjusted to be 7.4; the culture medium fluid is subpackaged in a sealed liquid bottle, and then sterilized in the high pressure; the finished product of the blood culture medium includes the obtaining process that 100 units of penicillase are added in each bottle through an injection syringe or not added, after the sterility test, the blood culture medium is reserved in the room temperature. Compared with the prior art, the present invention eliminates the long time heating dissolving and filter process, saves the preparation time and the filter material, and the operation issimple and convenient.

The present invention relates to a sterility test speical-purpose culture medium suitable for growth of fungi and for growth of bacteria. Said culture medium is formed from peptone, yeast extract powder, glucose, sodium chloride, potassium phosphate dibasic, bitter salt and water.

The invention relates to the technical field of medical instruments, in particular to a disposable three-cavity gastroscope and enteroscope sleeve which can prevent cross infection during gastroscopy. The technical scheme includes that firstly, a negative pressure suction forceps channel tube and a water and air injecting pipe are fixed on the outer side of a flexible pipe, thereby an independent water and air pipeline system, an independent negative pressure suction and forceps channel system, a gastroscope air and water eduction control system and a negative pressure suction and forceps channel eduction control system are formed, the complete disposable three-cavity gastroscope and enteroscope sleeve is formed, and gastroscope or enteroscope can be thoroughly isolated from a gastral cavity or an intestinal tract during gastroscopy or enteroscopy. Accordingly, defects of prior built-in pipelines of the gastroscope and the enteroscope are overcome, sterility tests of the gastroscope and the enteroscope are achieved, and the cross infection is completely eradicated.