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Foot-and-mouth disease virus antigen preservation technology

A virus and antigen technology, applied in the field of veterinary biological product preservation, can solve problems such as high cost, unsuitable for industrialized vaccine production, and temperature sensitivity

Inactive Publication Date: 2003-04-30
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no specialized technology in this area in China. Generally, virus antigens are frozen at -20°C. Although this storage condition is low in cost and in line with my country's national conditions, due to the poor tolerance of foot-and-mouth disease virus to adverse physical and chemical factors, Especially sensitive to temperature, therefore, it is easy to cause degradation and loss of effective viral antigens
Countries that have established FMD regional defense or their own national vaccine / antigen banks such as Canada, Mexico, the United States, Australia, New Zealand, Ireland, the United Kingdom, Norway, Sweden, the Netherlands, Finland, Germany, France, Italy, Thailand, FAO The Yatai Regional Animal Production and Health Committee mainly concentrates the virus antigen at a high power first, and then directly stores it in liquid nitrogen (-196°C). The preparation of vaccines with antigens still has good immunogenicity, but this technology has strict requirements on the entire production process, system, and facilities, and is expensive and has a small storage capacity, which is not suitable for the current and recent industrialized production of vaccines in my country

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] ① Preparation of protective agent

[0012] Use 0.5% hydrolyzed milk protein solution as the mother solution, add 4% glycerin, 4% peptone and 10% sucrose, mix thoroughly, sterilize under high pressure at 12 pounds for 15 minutes, and use 50% after the sterility test is negative Add it to the foot-and-mouth disease virus solution and store it at -20°C.

[0013] ②Effect analysis

[0014] The protective agent prepared in Example 1 is added to the foot-and-mouth disease virus liquid in a 50% amount, and the control virus without protective agent is established, then they are left standstill for 40hr and 50hr at 37°C simultaneously, and the infection of the virus is measured Sex titer (TCID 50 ), as a result, the foot-and-mouth disease virus liquid that added protective agent, to sensitive cell TCID 50 can still reach 10 -6.0 and 10 -3.0 , while the control virus without protective agent was reduced to 10 -1.67 and 0. As we all know, foot-and-mouth disease virus is ver...

Embodiment 2

[0016] ① Preparation of protective agent

[0017] Use 0.5% hydrolyzed milk protein solution as the mother solution, add 4% glycerin, 2% peptone and 5% sucrose, mix thoroughly, sterilize at 12 pounds for 15 minutes, and use 50% after the sterility test is negative Add it to the foot-and-mouth disease virus solution and store it at -20°C.

[0018] ②Effect analysis

[0019] The protective agent prepared in Example 2 is added to the foot-and-mouth disease virus liquid in a 50% amount, and the control virus without protective agent is established, and then they are left standstill for 40hr and 50hr at 37°C simultaneously to measure the infection of the virus Sex titer (TCID 50 ), as a result, the foot-and-mouth disease virus liquid that added protective agent, to sensitive cell TCID 50 can still reach 10 -4.5 and 10 -2.3 , while the control virus without protective agent was reduced to 10 -1.67 and 0.

Embodiment 3

[0021] ① Preparation of protective agent

[0022] Use 0.5% hydrolyzed milk protein solution as the mother solution, add 2% glycerin, 2% peptone and 10% sucrose, mix well, sterilize under high pressure at 12 pounds for 15 minutes, after the sterility test is negative, use 50% Add it to the foot-and-mouth disease virus solution and store it at -20°C.

[0023] ②Effect analysis

[0024] The protective agent prepared in Example 3 is added to the foot-and-mouth disease virus liquid in an amount of 50%, and the control virus without protective agent is established, and then they are left standstill for 40hr and 50hr at 37°C simultaneously to measure the infection of the virus Sex titer (TCID 50 ), as a result, the foot-and-mouth disease virus liquid that added protective agent, to sensitive cell TCID 50 can still reach 10 -5.0 and 10 -1.5 , while the control virus without protective agent was reduced to 10 -1.67 and 0.

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Abstract

A technique for preserving the antigen of foot-and-mouth disease virus includes such steps as preparing its protecting agent from hydrolytic actoprotein liquid, glycerine, peptone and cane sugar through proportional mixing and high-pressure sterilizing, sterility test, adding it to the foot-and-mouth disease virus liquid, and preserving at -20- -70 deg.C or in liquefied nitrogen (-196 deg.C).

Description

(1) Technical field [0001] The invention belongs to the technical field of preservation of veterinary biological products, and in particular relates to a preservation technique of foot-and-mouth disease virus antigen. (2) Technical background [0002] Foot-and-mouth disease is one of the most important animal diseases in the world. It is listed as the first class A disease by the International Office of Epizootics (OIE). . In order to effectively control and prevent foot-and-mouth disease, some countries that have eliminated or are controlling foot-and-mouth disease have established regional joint defense or their own national foot-and-mouth disease vaccine / antigen bank. Although our country successfully developed pig and cattle foot-and-mouth disease O-type inactivated vaccine in the late 1980s and used it for actual epidemic prevention, its quality and quantity are far from meeting the needs of actual epidemic prevention, and it has not yet established its own national va...

Claims

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Application Information

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IPC IPC(8): A61K39/135C12N7/01
Inventor 王永录张永光方玉珍蒋守田
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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