The invention discloses an ultralow-temperature
preservation technique of calluses of divaricate
saposhnikovia root. A technical divaricate
saposhnikovia root in-vitro ultralow-temperature preservation
system is established with calluses obtained by inducing divaricate
saposhnikovia root seeds as materials, and therefore, the high-quality
germplasm resources of the divaricate saposhnikovia root can be effectively protected. According to the technique, the ultralow-temperature preservation of the calluses of the divaricate saposhnikovia root is realized by virtue of the processes of
callus induction, pre-culture, loading,
dehydration, freezing, washing, re-culture, regeneration and the like; the technique can be taken as a method for preserving the
germplasm resources stably for a long time; besides, the economic cost of preservation can be reduced, the valuable and rare
germplasm resources can be effectively preserved and great help can be provided for
scientific study.