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Preserving, seedling-growing method of Chinese fan palm seedling

A technology for seeds and palmflower, applied in the field of plant germplasm resources preservation, can solve problems such as the loss of vitality of palmflower seeds, and achieve the effects of good application prospects, improvement and promotion, and easy operation.

Inactive Publication Date: 2006-07-12
中国科学院西双版纳植物热带园
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for long-term preservation and seedling cultivation of palmetto seeds in view of the deficiencies in the prior art, to solve the problem of rapid loss of vigor of palmetto seeds in the preservation process, so that the preserved palmetto seeds can recover growth , develop into normal seedlings, so as to realize the long-term preservation of germplasm resources of palm palm

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Choose the fruit sequence 160 days after flowering. At this time, the endocarp has fully developed, the exocarp is green, and the seeds have reached physiological maturity. The dehydration tolerance and ultra-low temperature tolerance are the highest, and ultra-low temperature storage is easy to succeed. Remove the exocarp, collect the seeds and put them into polyethylene bags, store them at 15°C for later use. Take the spare seeds within 1 week and sterilize them on the ultra-clean workbench, soak them in 75% alcohol for 2 minutes, then Soak in 0.1% mercury solution for 20 minutes, then rinse with sterile water 5 times, 6 minutes each time. Cut a knife from both sides of the embryo with branch shears, and then separate the embryo with a scalpel. According to the ratio of 100g activated silica gel for every 20 embryos, put the palm sunflower embryos in a small aluminum box with a lid and dehydrated with silica gel for 7 hours to reduce the water content of the embryos to 12...

Embodiment 2

[0021] Example 1 was repeated, with the following differences: Put the foxtail embryos in a small box with a lid and dehydrated with silica gel for 9 hours to reduce the moisture content of the embryos to 10%.

Embodiment 3

[0023] Example 1 was repeated, with the following differences: Put the foetus embryos in a small box with a lid and dehydrate them with silica gel for 5 hours to reduce the water content of the embryos to 15%.

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PUM

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Abstract

The invention discloses a method for Livistona seed preservation and grow seedling, sterilizing, embryo separating and dewatering for seeds firstly, then putting the embryo into the germ-free and low-temperature freezing storage tube and putting the tube to liquid nitrogen biological container for fast cooling. Getting the freezing storage tube from the liquid nitrogen when young sprout is needed, and putting the tube into warm water of 30-35 Deg C to make temperature recover to room temperature. Finally, culturing the embryo in the culture plate, and getting mature sprout after 2-3 months. The invention is characterized by the long-term preservation of Livistona seed, simple operation, low cost, almost 100% of the seed survival rate, greater than 95% of the sprout-producing rate, and good application prospect, and at the same the method can also promote the perfection of other seed preservation technique.

Description

Technical field [0001] The invention belongs to the technical field of preservation of plant germplasm resources, and specifically relates to a method for preservation and seedling raising of palm sunflower seeds. Background technique [0002] Palm sunflower is a palm plant, native to southern China. The whole body of the foxtail is a treasure. Its trunk can be used as a cane and umbrella handle. The leaves can be used to make sunflower fans, fur clothes, hats, and blues. Sunflower bones (middle veins) can be used to make brooms and toothpicks. Palmetto is also an excellent fiber plant, which can be used to make ropes, and the petiole skin can be woven into sunflower mats. The buds of the palm sunflower are edible, and the fruit, leaves and roots can be used as medicine (according to the fifth volume of "Chinese Higher Plants", Science Press, 1976). Because of its beautiful tree shape, it is widely used in garden landscaping, garden viewing and street trees. It is the most popula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01C1/00A01N3/00
Inventor 文彬宋松泉
Owner 中国科学院西双版纳植物热带园
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