46 results about "Bacterial lysate" patented technology

The present invention relates to methods and compositions for treatment of microbial infections and for the enhancement of resistance to infection. The invention comprises administration of an effective amount of bacterial lysate compositions for the treatment of pathological conditions of microbial infections. The present invention can also be used to enhance the immune system to prevent infections by the administration of an effective amount of the compositions.

The invention discloses a kit for extracting nucleic acid from bacteria by using a paramagnetic particle method and an extracting method. The kit comprises a bacteria lysate, a magnetic bead binding solution, a magnetic bead scrubbing solution and a nucleic acid eluent. The bacteria lysate comprises sodium dodecyl sulfate, ethylenediaminetetraacetic acid, tri-hydroxymethyl aminomethane and sodium chloride; the magnetic bead binding solution comprises polyethylene glycol-8000 and sodium chloride; the magnetic bead scrubbing solution comprises ethanol; and the nucleic acid eluent comprises tri-hydroxymethyl aminomethane and ethylenediamine tetraacetic acid. The kit comprises the unique bacteria lysate which has a strong lysis function for the bacteria; and the kit can be used for manual extraction and instrument extraction by using a commercially available nucleic acid isolation machine, high-purity and high-concentration bacteria nucleic acid can be extracted.

The present invention relates to the cosmetic treatment of greasy skin and / or skin that tends to be greasy with a lysate of at least one microorganism of the genus Bifidobacterium species and / or a fraction thereof, as an active.

Disclosed herein are compositions and methods for regulating redox status and / or reducing oxidative stress in a subject, the methods and compositions comprising TLR agonists comprising bacterial lysates and / or lysate fractions. Also disclosed are compositions and methods comprising bacterial lysates and / or lysate fractions formulated or administered in combination with one or more other therapeutic or pharmaceutical agents.

The invention relates to the technical field of detecting and processing of oilfield water samples, and in particular relates to a rapid detecting method of sulphate reducing bacteria and a kit for detecting. The rapid detecting method disclosed by the invention comprises the following steps: firstly, pre-processing and removing impurities in a water sample to be tested, then adding a bacteria lysis solution into the water sample to sufficiently split and release APS thallus in the water sample, adding a reaction substrate, catalyzing the reaction substrate by using enzyme to carry out oxidization-reduction reaction, adding a colour developing substrate into the reaction system, combining the reaction product catalyzed by enzyme with the colour developing substrate, and thus obtaining a coloured reaction end-product. The content of SRB (Sulphate Reducing Bacteria) in the water sample to be tested is obtained according to reaction colours. According to the invention, a device for detecting SRB and a reagent are standardized to form a kit according to the detection method, so that various construction fields are convenient to detect; the rapid detecting method of sulphate reducing bacteria and the kit disclosed by the invention are capable of effectively eliminating impurity interference in the oilfield water sample, greatly shortening the detection period and greatly increasing the detection accuracy and sensibility.

The present invention describes isolation of plasmid DNA from bacteria. The addition of dyes to the alkaline lysis based purification buffers (P1, P2, and P3) allows for improved visual monitoring of the steps of preparing a bacterial lysate filtrate coupled to filtration or spin-column chromatography. The method comprises the suspending of the bacterial cells with buffer P1 (suspension is red / pink); lysing the bacteria with buffer P2 (suspension goes from red / purple color to translucent / purple); precipitating cellular debris with buffer P3 (solution becomes yellow with debris suspension); centrifuging or filtering to product a lysate filtrate; binding the lysate filtrate to a DNA binding matrix; washing; and isolating the plasmid via chromatography. The yield and quality of plasmid DNA is improved due to more consistent lysis. Errors in buffer addition are reduced by visualizing the color as buffers are added and also of changes in color of the preparation at each step.

The invention relates to a flocculant production method, in particular to a production method for microbial flocculants, and aims to solve the problems of long production cycle of existing flocculant production methods and poor flocculation effect of flocculants. The method includes 1, picking acinetobacter and inoculating the same into a rich culture medium to obtain an active bacterium solution, and inoculating the bacterium solution into a flocculation culture medium; 2, performing fermentation cultivation to obtain a fermentation broth; 3, centrifuging the fermentation broth to collect thallus sediments, adding a bacteria lysate into the sediments, performing ultrasonic disruption, collecting a supernatant through centrifugation, adding CTAB (cetyltrimethyl ammonium bromide), collecting sediments after standing and centrifuging, adding absolute ethyl alcohol and acetone, collecting a supernatant through centrifugation, adding ethyl alcohol, collecting sediments through centrifugation, and washing the sediments which are the microbial flocculants. The production method applied to the field of waste water treatment is short in production cycle, and the flocculation effect of the microbial flocculants is good.

The invention relates to a double-magnetic-bead method based centrifugation-free extraction kit for plasmid DNA (deoxyribonucleic acid). The kit accommodates an impurity-removing magnetic bead suspension liquid, an extraction magnetic bead suspension liquid, a bacterium lysis solution, a binding buffer solution, a cleaning solution and an eluent which are separately subpackaged, wherein impurity-removing magnetic beads are dispersed in the impurity-removing magnetic bead suspension liquid and surfaces of the impurity-removing magnetic beads are at least modified with styrene, divinyl benzene and iminodiacetic acid; extraction magnetic beads are dispersed in the extraction magnetic bead suspension liquid and surfaces of the extraction magnetic beads are modified with silicon hydroxyl. According to development of the impurity-removing magnetic beads, impurities such as cell walls, protein and the like are removed after bacterium lysis, the plasmid DNA in a liquid supernatant is reserved, so that the centrifugation step in a traditional process is replaced, and the operation is more efficient and faster; when the kit is in use, the buffer solution, the magnetic bead suspension liquid and the like can be subpackaged in corresponding pore plates of a nucleic acid extraction instrument, and requirements for automation and high throughput are met; the extracted plasmid DNA has high purity and complete fragments.

The invention relates to a bacterial lysate and a kit for extracting plasmid DNA and a method for extracting the plasmid DNA, and belongs to the technical field of biology. The bacterial lysate for extracting the plasmid DNA comprises lysis buffer, lysozyme and ribonuclease A(RNaseA); wherein100 mL of the lysis buffer comprises 1.5-10 g of sucrose, 0.7-2.2 g of ethylene diamine tetraacetic acid (EDTA), 0.3-0.9 g of tri(hydroxymethyl) amino methane hydrochloride, 2.7-5.4 g of NH4Cl, 0.1-2.0 mL of 5% Triton X-100, 0.2-0.6 g of CaCl2, 0.5-8 g of guanidine hydrochloride, 0.5-3 g of guanidinium isothiocyanate and the balance of water. The kit for extracting the plasmid DNA comprises the bacterial lysate, rinsing solution and eluant and is low in cost, high in purity of extracted plasmid and high in applicability.

The invention discloses a method for extracting bacterium genomic DNA by using magnetic nanoparticles, belonging to the technical field of nanomaterials and molecular biology. The method comprises the steps of: preparing a magnetic nanoparticle aggregate, preparing a bacterium lysing solution, preparing and adding an absorption buffer solution, absorbing with a magnet, rinsing with 70 percent alcohol, and eluting with a TE buffer solution to obtain a bacterium genomic DNA solution. Compared with the traditional phenol / chloroform DNA extracting method, the method for extracting the bacterium genomic DNA by using magnetic nanoparticles has the advantages of short experiment time, simpleness in operation, high extraction efficiency, rapidness, convenience, sensitivity, high yield and the like; and the extracted bacterium genomic DNA can be completely used for subsequent experiments, such as DNA hybridization, PCR (Polymerase Chain Reaction), and the like, and provides the basis for the subsequent research and analysis.

The invention provides a reagent and a method for extracting plasmids. The reagent for extracting plasmids comprises a bacteria lysis solution, an endotoxin removing grain solution, an endotoxin removing solution, a plasmid combining solution, a cleaning solution, an eluant and the like. According to the reagent and the method for extracting the plasmids provided by the invention, the operation steps are simple, the time for extracting is short, the endotoxin content in the extracted plasmids can be effectively reduced, the content of endotoxin in the extracted plasmids can be reduced to be lower than 0.1EU / mu g to satisfy the clinical criteria and the obtained plasmids have high DNA (Deoxyribonucleic Acid) yield and good purity.

The invention discloses a viral / bacterial lysate and a fluorescent quantitative PCR detection method and belongs to the field of biological detection. The lysate is prepared from NaCl, triton X-100, lithium dodecyl sulfate, EDTA-2Na and purified water through uniform mixing. The PCR reaction process is free of a DNA purification process. The nucleic acid extraction and amplification detection of the sample are carried out in a reaction tube without separation or purification processes such as heating, centrifugation and shocking. The operation is simple and convenient and realizes a low cost. The method has very low requirements on an operator, greatly reduces detection time and is especially suitable for bedside diagnosis of serious diseases and field inspection and quarantine work in the eruption of a major epidemic.

Disclosed are a bacteriologically-modified whole-cell tumor vaccine and a method of making the same. The method includes: lysing bacteria at logarithmic growth phase to obtain a bacterial lysate; mixing the bacterial lysate with an excessive amino compound solution to aminate the bacterial lysate in the presence of EDC; mixing the aminated bacterial lysate with the tumor cells for a certain period of time to produce bacteriologically-modified tumor cells; and inactivating the bacteriologically-modified tumor cells to produce the bacteriologically-modified whole-cell tumor vaccine. The bacteriologically-modified whole-cell tumor vaccine has been demonstrated to have desirable therapeutic effect in tumor model mice.

The present invention relates to the cosmetic treatment of greasy skin and / or skin that tends to be greasy with a lysate of at least one microorganism of the genus Bifidobacterium species and / or a fraction thereof, as an active.

This invention relates to an immunogenic composition comprising a bacterial extract containing at least one expression products of the vrg genes of a strain of Bordetella chosen from B. pertussis, B. parapertussis, or B. bronchiseptica. The invention also relates to a method for producing the extract, comprising culturing the bacteria on blood medium to obtain isolated nonhemolytic colonies; inoculating cells of one or more colonies in liquid medium to give a suspension of cells; separating the cells from the liquid medium after culture; suspending the separated cells in a buffer comprising urea for at least an amount of time sufficient to form a bacterial lysate; and separating intact cells and insoluble material from soluble material, wherein the extract comprises the soluble material.

The invention relates to a preparation method using bacterial lysate as inactivated vaccine adjuvant. The preparation method comprises the following steps: step 1, respectively putting corynebacterium pseudotuberculosis and rhodococcus equi into an LB culture medium for culturing at 37 DEG C, and harvesting thalli through a centrifugal machine; step 2, diluting each thallus to 8.0* 10<9> cfu / mL by using sterilized normal saline, and crushing the bacterial liquid by using a high-pressure homogenizer; and step 3, after sterility test, mixing the split products of corynebacterium pseudotuberculosis and rhodococcus equi with the two bacteria inactivated by formaldehyde according to a ratio of 1: 1 to obtain the inactivated vaccine added with the adjuvant. The preparation method has the characteristics of safety, high efficiency, strong immune enhancement capability and low cost, and is suitable for producing various bacterial inactivated vaccines.

The invention discloses an ELISA (Enzyme-linked Immuno Sorbent Assay) antigen detection kit of animal echinococcosis granulosa. The detection kit is prepared from an anti-EgAgB8 / 2 protein monoclonal antibody pre-coated ELISA plate, a sample diluting solution, a positive control solution, a negative control solution, an enzyme conjugate, a washing solution, a substrate color developing solution anda stopping solution. An anti-EgAgB8 / 2 protein monoclonal antibody is prepared through the following method: firstly, amplifying EgAgB8 / 2 gene cDNA (complementary Deoxyribonucleic Acid) by utilizing RT-PCR (Reverse Transcription-Polymerase Chain Reaction) and cloning an EgAgB8 / 2 gene; constructing a prokaryotic expression vector pET / EgAgB8 / 2 and expressing in escherichia coli BL21(DE3); inducing the expression of the prokaryotic expression vector by utilizing IPTG (isopropyl-beta-d-thiogalactoside); centrifuging and collecting thalli; after dissolving with a bacterium lysis solution and carrying out ultrasonic crushing; centrifuging and collecting supernatant; purifying by utilizing Ni-NTA glue; immunizing a BALB / c mouse by utilizing the purified EgAgB8 / 2 protein, so as to obtain a hybridoma cell line capable of stably secreting the anti-EgAgB8 / 2 protein monoclonal antibody; selecting the excellent monoclonal antibody to produce an antibody and purifying. The kit disclosed by the invention can be used for qualitative detection of an animal echinococcosis granulosa antigen and has the advantages of stable antibody source, good specificity, high sensitivity and good repeatability.

The invention provides a reagent and a method for extracting plasmids. The reagent for extracting plasmids comprises a bacteria lysis solution, an endotoxin removing grain solution, an endotoxin removing solution, a plasmid combining solution, a cleaning solution, an eluant and the like. According to the reagent and the method for extracting the plasmids provided by the invention, the operation steps are simple, the time for extracting is short, the endotoxin content in the extracted plasmids can be effectively reduced, the content of endotoxin in the extracted plasmids can be reduced to be lower than 0.1EU / mu g to satisfy the clinical criteria and the obtained plasmids have high DNA (Deoxyribonucleic Acid) yield and good purity.

The invention discloses an echinococcosis granulosa ELISA antibody detection kit. The detection kit comprise an EgAgB8 / 2 recombinant antigen pre-coating enzyme label plate, a sample weak solution, positive contrast liquid, negative contrast liquid, an enzyme conjugate, washing liquid, a substrate coloring solution and a stopping solution. A method for preparing the EgAgB8 / 2 recombinant antigen comprises the following steps: using RT-PCR for amplifying EgAgB8 / 2 gene cDNA, cloning an EgAgB8 / 2 gene, constructing a prokaryotic expression vector pET-EgAgB8 / 2, and performing expression in escherichia coli BL21(DE3), using the IPTG-induced prokaryotic expression vector for expression, performing centrifugation to collect thalline, using a bacteria lysate for dissolving, and performing ultrasonicfragmentation, performing centrifugal collection of a supernatant, and using a Ni-NTA glue for purification. The kit takes recombinant protein EgAgB8 / 2 as antigen to detect an animal echinococcosis granulosa antibody, can be used for qualitative detection of the animal echinococcosis granulosa antibody, and has the advantages of stable antigen source, good specificity, high sensitivity, and good repeatability.

An expression carrier for preparing the recombinant tryptophanase is pEVT (CCTCC M202038). The process for preparing said expression carrier and the process for purifying bacterial spawn and preparing tryptophanase protein are disclosed. As the tryptophanase fusion protein expressed by said recombinant carrier contains 6 continuous histidines, it is easy to bind the Ni-affinity fixed histidine-protain chromatographic column and the histidine does not affect the activity of target protein, so resulting in high recovery rate and purify of target protein (more than 60% and more than 95%) and low cost.

RNA, preferably messenger RNA, is purified by use of selective precipitation, preferably by addition of compaction agents. Also included is a scaleable method for the liquid-phase separation of DNA from RNA. RNA may also be recovered by fractional precipitation according to the invention. We have discovered that specific classes of compounds are unexpectedly potent in causing selective precipitation of DNA away from RNA, at low concentrations and in the presence of relatively elevated ionic strength. Modification of the selective removal of DNA can also remove both RNA and DNA, leaving behind a mixture which is advantageous for the further purification of, e.g., proteins. Additional aspects of the invention include mini-preps, preferably of RNA or of plasmid and chromosomal DNA to obtain sequenceable and restriction digestible DNA in high yields in multiple simultaneous procedures. Still further aspects disclose enhanced stripping of the compaction agent by a stripping method comprising high salt addition and pH shift, and combinations of these techniques. RNA Abstract Additionally, a new approach to the isolation of RNA from bacterial lysates employs selective precipitation by compaction agents. The above techniques can be enhanced by use of phase transfer catalysts (PTCs), most preferably selected polyamines of U.S. Pat. No. 6,617,108 polyamines which are quaternary compounds. (The use of PTCs in biotechnical processes is not evident in the literature, see e.g. www. / ptorganics.com as of 27 Jan. 2005.)

The present invention concerns a method for the cosmetic caring of the skin and / or mucosa, comprising applying a postbiotic composition comprising at least one postbiotic and at least one bacteriocin and / or endolysin, wherein said postbiotic is preferably a bacterial lysate preferably obtained from bacteria heterologously expressing said at least one bacteriocin and / or endolysin and wherein said postbiotic and said at least one bacteriocin and / or endolysin have a synergistic effect in the cosmetic caring method.

The present invention is to obtain a novel gene that encodes a protein having a function of promoting acetic acid bacterial growth in the presence of a high concentration of acetic acid; to enhance acetic acid bacteria's function of promoting growth and thereby to develop acetic acid bacteria with an improved fermentation ability in the presence of a high concentration of acetic acid; and to provide a method for efficiently producing vinegar that contains a high concentration of acetic acid in a short time using the acetic acid bacterium. A novel gene having a function of improving the function of promoting growth at a practical level was cloned from acetic acid bacteria for practical use, belonging to the genus Gluconacetobacter, by a method comprising exposing acetic acid bacterial lysate to the presence of a high concentration of acetic acid; obtaining a gene of a protein that was not insolubilized but remained soluble; and thereby obtaining a gene having a function of promoting acetic acid bacterial growth in the presence of acetic acid. When cultured in the presence of acetic acid and ethanol, a transformant of acetic acid bacteria transformed with the gene was confirmed to have a significant effect of promoting growth.

The invention relates to probiotic bacteria and particularly, although not exclusively, to anti-bacterial compositions derived from probiotic bacteria. Disclosed are compositions comprising material secreted from probiotic bacteria, uses thereof, and methods using the compositions. Particularly contemplated are compositions comprising material secreted from Lactobacillus rhamnosus, and uses thereof.

The invention relates to a kit and a method for extracting bacterial plasmids by an alkali lysis method, and belongs to the technical field of biology. According to the kit for extracting the bacterial plasmids by the alkali lysis method, inorganic salts LiCl and NH4Ac are added into bacterial suspension, so that RNA (Ribonucleic Acid) in a bacterial lysis solution is easy to precipitate and remove, the preservation time of the extraction solution is long, and the required expenditure is low; the glucose concentration is increased in the bacterial lysis solution, and high-concentration guanidine isothiocyanate is added in the plasmid renaturation solution, so that the degradation of the plasmids can be prevented, and the integrity of the extracted plasmids is ensured. Meanwhile, the silica gel membrane technology is adopted, the time needed for collecting plasmids in the solution is short, the contact time of the plasmids and the solution is shortened, small plasmids can be extracted, large plasmids can also be extracted, and the requirements for sequencing, enzyme digestion and other operations of molecular biology experiments can be met.

The present invention is to obtain a novel gene that encodes a protein having a function of promoting acetic acid bacterial growth in the presence of a high concentration of acetic acid; to enhance acetic acid bacteria's function of promoting growth and thereby to develop acetic acid bacteria with an improved fermentation ability in the presence of a high concentration of acetic acid; and to provide a method for efficiently producing vinegar that contains a high concentration of acetic acid in a short time using the acetic acid bacterium. A novel gene having a function of improving the function of promoting growth at a practical level was cloned from acetic acid bacteria for practical use, belonging to the genus Gluconacetobacter, by a method comprising exposing acetic acid bacterial lysate to the presence of a high concentration of acetic acid; obtaining a gene of a protein that was not insolubilized but remained soluble; and thereby obtaining a gene having a function of promoting acetic acid bacterial growth in the presence of acetic acid. When cultured in the presence of acetic acid and ethanol, a transformant of acetic acid bacteria transformed with the gene was confirmed to have a significant effect of promoting growth.

The present invention relates to methods and compositions for treatment of microbial infections and for the enhancement of resistance to infection. The invention comprises administration of an effective amount of bacterial lysate compositions for the treatment of pathological conditions of microbial infections. The present invention can also be used to enhance the immune system to prevent infections by the administration of an effective amount of the compositions.

The present invention relates to a producing and purifying method of soluble recombinant COQ5 protein, which is expressed in soluble form by E. coli, under native conditions. The method is characterized by pre-treating bacterial lysate with low concentration of ionic detergent, such as SDS, before purification; and the purifying method is performed under native condition without using urea to avoid the problems of requiring lengthy processes to remove urea in purified protein solution or re-aggregation and precipitation of protein after removal of urea.