Fast dissolution preparation method for blood culture medium
A preparation method and culture medium technology, which are applied in the field of biomedicine, can solve the problems of turbidity of culture medium, troublesome preparation of blood culture medium, poor effect, etc., and achieve the effects of facilitating mass production, saving preparation time and saving filter materials.
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[0011] Weigh medium raw materials: peptone 10g, beef extract 3g, sodium chloride 5g, sodium citrate 3g, nucleic acid 2g, agar 0.5g, set aside.
[0012] To prepare the quick solution:
[0013] Instant solution 1: take 24.6g of magnesium sulfate and add 100ml of distilled water to dissolve and make 24.6% magnesium sulfate solution for subsequent use;
[0014] Instant solution 2: Weigh 0.5g of p-aminobenzoic acid and add 100ml of distilled water to prepare a 0.5% p-aminobenzoic acid solution for later use;
[0015] Mixing: Put the medium material into a large Erlenmeyer flask, then take 20ml of fast solution 1 and 10ml of fast solution 2 and add it to the medium raw material, stir it into a paste, then add 1000ml of distilled water and shake to mix, let it stand for 3-5 In minutes, a clear and transparent culture medium can be obtained, and the dissolution rate is fast;
[0016] Adjust the pH value: adjust the pH value of the culture medium to 7.4 with a hydrogen ion colorimete...
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