Separation and purification method of endophytic fungi of solidago canadensis

Abstract

The invention relates to a separation and purification method of endophytic fungi of solidago canadensis. The method comprises the following steps of: 1, collecting stems and leaves of the solidago canadensis; 2, carrying out solid culture and sterility test on the stems and the leaves; and 3, carrying out isolated culture on the endophytic fungi of tissues of the solidago canadensis to obtain the endophytic fungi of the solidago canadensis. A fungus colony has silk velvet texture and a villous surface, does not generate pigment and is light olivaceous and black brown at the back; and the gene complex of the endophytic fungi ITS of the solidago canadensis is composed of 602 basic groups (bp). The geomicrobiology status of a bacterial strain of chaetomiumglobosumyt03 with broad-spectrum antibacterial activity is determined, and the endophytic fungi is found to have relatively remarkable role in inhibiting mycelial growth of six provided plant pathogenic fungi through antibacterial test of the fermentation liquid of the chaetomiumglobosumyt03.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for separating and purifying microorganisms whose fermented products have the activity of inhibiting plant pathogenic fungi, that is, a method for separating and purifying endophytic fungi from Solidago canadensis. Background technique [0002] The main means of controlling crop diseases, insect pests and weeds in the process of agricultural production is the control of chemical pesticides, which play a positive role in reducing the damage of diseases, insect pests and weeds and ensuring high grain yields. However, due to the interdependence and mutual restraint among natural organisms, excessive reliance on the poisonous effect of chemical pesticides has led to the phenomenon of blind and indiscriminate use of pesticides in some areas, especially the use of some pesticides with long residual effects and high toxicity, resulting in a series of food safe...

AI Technical Summary

Problems solved by technology

However, due to the interdependence and mutual restraint among natural organisms, excessive reliance on the poisonous effect of chemical pesticides has led to the phenomenon of blind and indiscriminate use of pesticides in some areas, especially the use of some pesticides with long residual effects and high toxicity, resulting in a series of food safety issues. , human health and living environment issues

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