Aeromonas hydrophila inactivated vaccine and preparation thereof

Abstract

The invention relates to a fish vaccine and a preparation method thereof, in particular to an aeromonas hydrophila inactivated vaccine and the preparation thereof. The aeromonas hydrophila inactivatedvaccine is prepared by the following concrete steps: separating out a pathogen from a sick fish, determining the pathogen as an aeromonas hydrophila through morphology, virulence factors, biochemicalreaction and gene sequencing; rejuvenating the strain, selecting an optimum condition for cultivation; then selecting the optimum condition for inactivation to prepare the aeromonas hydrophila inactivated vaccine, and conforming the aeromonas hydrophila inactivated vaccine to be certificated through sterility test and security test. The inactivated vaccine is prepared from a pathogenic bacterialstrain separated from a sick fish body, has a simple operating method, strong pertinency, strong protective function, good immunological effect and low cost of the used material, and is suitable for mass production. The invention provides a practical method for preventing and curing the bacteremic septicemia of carps.

Description

technical field [0001] The invention relates to a fish vaccine, in particular to an inactivated Aeromonas hydrophila vaccine and its preparation. Background technique [0002] Aeromonas hydrophila widely exists in fresh water, soil, sewage, sludge and feces, and can be divided into pathogenic strains and non-pathogenic strains. The infection spectrum of pathogenic strains is very wide, and it is the main pathogenic bacteria of freshwater fish. Bacterial septicemia caused by this bacterium is one of the most harmful fish types in the history of fish farming in my country, the largest age range of harmful fish, the widest endemic area, the longest endemic season, the most types of fish farming waters, and the most serious losses. an acute infectious disease. The disease is currently mainly controlled by antibiotics and chemical drugs, but long-term use of antibiotics is easy to produce drug resistance, chemical drugs will cause residues, pollute water quality, and destroy eco...

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Problems solved by technology

Studies have shown that the disease prevention effect of these vaccines has greatly surpassed other control measures such as antibiotics and chemicals, but these vaccines also have some problems, such as: unstable immune effect, poor safety, poor preparation technology, and high cost. There are still some difficulties in the widespread application of these vaccines in actual production

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