PoSaV GIII type strain whole genome amplification primer and amplification method thereof
A technology for whole-genome amplification and amplification primers, which is applied in the field of whole-genome amplification primers and amplification of PoSaVGIII strains, and can solve problems such as the complexity of PoSaV diagnosis and control, and the difficulty of eliminating or preventing natural infection of piglets
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0059] 1 Materials and methods
[0060] 1.1 Materials
[0061] 1.1.1 Sample source
[0062] Pig Sapporo virus-positive disease materials were isolated from fecal samples of pigs with diarrhea, provided by the Animal Infectious Disease Laboratory, School of Veterinary Medicine, Yunnan Agricultural University. The positive disease materials were numbered YNJD158 and YNAN326.
[0063] 1.1.2 Main reagents
[0064] TRipure Reagent total RNA extraction reagent was purchased from Beijing Biotech Co., Ltd.; columnar plasmid DNA mini-extraction kit and gel recovery kit were purchased from Sangon Bioengineering (Shanghai) Co., Ltd.; Easyscript RT / RI Enzyme MIX, 2× Es Reaction MIX, 2×TranTaq HiFi PCR Super Mix II were purchased from Beijing Quanshijin Biotechnology Co., Ltd.; pMD18-T Vector, E.coli DH5α Competent Cells, restriction enzymes, etc. were purchased from Bao Bio (Dalian) Co., Ltd.; other Reagents were provided by the Animal Infectious Diseases Laboratory, School of Veterina...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com