44 results about "Bacterial septicemia" patented technology

The invention relates to a fish septicemia pathogenic aeromonas veronii attenuated live vaccine and application thereof. The fish septicemia pathogenic aeromonas veronii attenuated live vaccine has the advantages that by modifying the gene of wild aeromonas veronii and importing into a specific pepaptamer SNP-L1, the toxicity is reduced, and an aeromonas veronii attenuated strain L1 is obtained; the live vaccine prepared by the attenuated strain L1 is injected into zebra fish via abdominal cavity, the later immunity protection rate is 65percent, the fish bacterial septicemia can be effectively prevented, the certain application value is realized, and the development prospect of attenuated live vaccine is realized.

The invention relates to a special feed additive for a sturgeon. The special feed additive is prepared from the following raw materials by weight: 3 to 5 parts of phoenix-tail fern, 2 to 4 parts of Chinese lobelia herb, 3 to 5 parts of weeping forsythia capsule, 2 to 4 parts of perilla, 5 to 8 parts of garlic, 4 to 7 parts of aniseed, 2 to 4 parts of licorice, 2 to 5 parts of white peony root and 10 to 15 parts of a carrier. With the special feed additive for the sturgeon provided by the invention, economic benefits in artificial breeding of the sturgeon are obviously improved, occurrence of diseases, especially occurrence of bacterial septicemia, a bacterial hemorrhagic disease and bacterial enteritis, during artificial breeding of the sturgeon is substantially reduced, the survival rate of the sturgeon is increased by 12 to 15%, growth of the sturgeon is promoted, and quality of the sturgeon is enhanced. Moreover, prices of the raw materials are low, so production and use cost of the feed additive is low, which is favorable for saving of cost.

The invention relates to a construction method of a grass carp anti-bacterial septicemia strain. The construction method comprises the following steps: a) selecting parent fishes, applying PIT (Point In Time) electronic tags to the parent fishes, when the parent fishes are mature in sexual glands, injecting hormone to accelerate maturation, performing natural fertilization, hatching fertilized ova to be baby fishes, cultivating fingerlings, and cultivating one-year-old fishes and two-year-old fishes in the same pool; b) respectively using pathogenic bacteria to infect offspring artificially in the baby fish phase and the 2-year-old fish phase, observing for 19-23 days after infection, and taking the residual individuals as a disease-resistant colony; c) adopting a paternity test method to perform progeny test on the obtained disease-resistant colony, selecting the parent fishes with strong disease resistance from the parent fishes, and in the second year, matching and propagating the parent fishes with strong disease resistance, and constructing the grass carp anti-bacterial septicemia strain. The construction method disclosed by the invention avoids the environment effect, can ensure that the obtained disease-resistant parent through screening is the real one with excellent heredity, ensures that the cultivated disease-resistant strain is of good disease-resistant performance; meanwhile, the method is easier to operate, and suitable for large-scale breeding for disease resistance.

The invention discloses a grass carp bacterial septicemia and red skin disease combined vaccine and a preparation method thereof, using β-propiolactone as an inactivator to develop safe and efficient grass carp Aeromonas hydrophila septicemia and Pseudomonas aeruginosa Bacterial erythroderma dual white oil inactivated vaccine can effectively control the occurrence of bacterial septicemia and erythroderma in grass carp. After immunizing grass carp, it can stimulate the expression of IgM, IL-1β, IFN, Mx, C3 and TLR3 immune-related genes in the spleen and head kidney tissue of the fish, and the vaccine can produce a relative immune protection rate of 80-90% for grass carp , the relative protection rate can still maintain 50% 1 year after immunization; the invention is easy to operate, safe and effective, and two diseases can be prevented by one immunization, which not only reduces the use of antibiotics and chemical drugs, but also reduces the impact on the breeding environment and ecological environment, etc. The pollution and destruction of the external environment ensure the food safety and water ecological safety of grass carp.

The invention discloses the use of Aeromonas hydrophila bacteriophage AH10-Phage-QY01 in preparing a drug for treating or preventing and controlling aquaculture bacterial diseases, and belongs to the technical field of aquaculture bacterial disease prevention and control. The bacteriophage AH10-Phage-QY01 is deposited in the China Center for Type Culture Collection, the deposit number is CCTCC NO: M2019109, and also includes the use of the bacteriophage AH10-Phage-QY01 in the preparation of bacterial septicemia caused by Aeromonas hydrophila, which can be Used alone or in combination with antibiotics. The present invention utilizes the ubiquity of phage and its high specificity to host bacteria to screen out Aeromonas hydrophila phage AH10-Phage-QY01 from the natural environment for the first time against pathogenic bacteria, which is used to inhibit and lyse pathogenic bacteria, To treat or prevent bacterial fish diseases such as bacterial sepsis caused by the pathogenic bacteria.

The invention discloses an application of chitosan in preparing drugs for preventing and curing bacterial septicemia of silkworm. The drug can obviously reduce the risk of infection of the bacterial septicemia of silkworm and has obvious curative effect. The adoption of the drug for preventing and curing bacterial septicemia of silkworm is easy to operate and has lower cost; and more importantly,the preventing and curing links can not generate any threat to the health of workers working for preventing and curing.