SNP marker associated with bacterial septicemia of schizothorax prenanti and application of SNP marker

A Schizothorax and bacterial technology, applied in the SNP markers associated with Schizothorax bacterial sepsis and its application fields, can solve problems that need to be discovered, and achieve simple operation, high accuracy, and large throughput Effect

Inactive Publication Date: 2017-07-21
SOUTHWEST UNIVERSITY
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  • Description
  • Claims
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Problems solved by technology

However, no SNPs related to disease resistance in Schizothorax have been reported, and SNP markers that can be effectively used in the breeding of disease resistance-related traits in Schizothorax remain to be discovered

Method used

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  • SNP marker associated with bacterial septicemia of schizothorax prenanti and application of SNP marker
  • SNP marker associated with bacterial septicemia of schizothorax prenanti and application of SNP marker

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Embodiment Construction

[0024] Embodiments of the technical solution of the present invention will be described in detail below. The following examples are only used to illustrate the technical solutions of the present invention more clearly, and therefore are only examples, rather than limiting the protection scope of the present invention.

[0025] 1.1 Sources of Schizothorax population samples

[0026] The fish to be tested were taken from the same batch of 13-month-old Schizothorax artificially bred in the same batch of cold-water fish farms in Mount Emei. 178 healthy Schizothorax were randomly selected, with a weight of about 100g / tail. The Schizothorax was injected with half-lethal concentration of Aeromonas hydrophila bacteria, the individuals who died within 7 days and those with typical symptoms were considered susceptible individuals, and the individuals without infection symptoms were identified as resistant individuals. Among them, there were 109 individuals in the susceptible group and ...

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Abstract

The invention provides an SNP marker associated with bacterial septicemia of schizothorax prenanti and application of the SNP marker. A nucleotide sequence of the SNP marker is shown in SEQ ID NO. 1; a 779th base of the sequence from 5' terminus is C or T. At an SNP site of the invention, the probability that the schizothorax prenanti having genotype CT is infected with bacterial septicemia is significantly higher than that of homozygous CC type individuals. Therefore, whether the schizothorax prenanti is easily infected with bacterial septicemia can be determined effectively by detecting the SNP of the schizothorax prenanti. The CT genotype individuals are knocked out in breeding of parents; and the marker of the invention is used for auxiliary breeding to accelerate a cultivation process of excellent disease-resistant varieties of the schizothorax prenanti.

Description

technical field [0001] The present invention relates to a SNP marker associated with Schizothorax bacterial sepsis and its application, in particular to a SNP marker associated with Schizothorax bacterial sepsis, a primer pair and a kit for detecting the aforementioned SNP marker, and the aforementioned SNP Use of markers, primer pairs and kits in Schizothorax chrysothorax selection, and a method for detecting bacterial sepsis in Schizothorax chrysothorax. Background technique [0002] Full mouth Schizothorax ( Schizothorax prenanti ) is one of the important cold-water fish in my country. Its tender meat, delicious taste and high nutritional value are favored by consumers. It has become a valuable economic fish in the upper reaches of the Yangtze River in my country. my country used to have abundant wild resources of Schizothorax, but in recent years, due to overfishing, construction of power stations, and water pollution, the wild resources of Schizothorax have been severe...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 叶华罗辉张争世周朝伟朱成科
Owner SOUTHWEST UNIVERSITY
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