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Disease-resistant Chinese herbal medicine and egg yolk antibody compound additive for siniperca chuatsi, and feeding method

A compound additive, egg yolk antibody technology, applied in the direction of antibodies, antibacterial drugs, pharmaceutical formulations, etc., can solve the problems that drugs and immune preparations cannot be fed, difficult to control diseases, etc., achieve good disease resistance, enhance immunity, The effect of enhancing resistance

Inactive Publication Date: 2019-01-15
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, it is difficult to control the occurrence and prevalence of diseases caused by Aeromonas hydrophila in mandarin fish aquaculture production, especially mandarin fish feed on live fish (heterogenetic gibel crucian carp and red-eyed trout, etc.), and drugs and immune preparations cannot be passed through. Compound Feed Feeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, a kind of mandarin fish disease-resistant Chinese herbal medicine and yolk antibody compound additive, described compound additive is made up of three kinds of compositions of following weight ratio,

[0027] (1) Anti-Mandarin fish bacterial sepsis egg yolk antibody solution 1;

[0028] (2) Three flavors of Chinese herbal medicine extract 1;

[0029] (3) β-cyclodextrin accounts for 5% of the total weight of the compound additive;

[0030] The preparation method of described anti-mandarin fish bacterial sepsis egg yolk antibody liquid is as follows:

[0031] ① The strong pathogenic pathogen Aeromonas hydrophila ( Aeromonas Hydrophila ) G3 strains were inoculated on ordinary nutrient broth medium, cultured on a shaker at 28°C for 24 hours, added formaldehyde with a final volume concentration of 0.3% at 28°C for 48 hours, and centrifuged to obtain inactivated bacteria;

[0032] ② Soak Astragalus membranaceus in distilled water for 30 minutes, decoct, f...

Embodiment 2

[0036] Embodiment 2, a kind of mandarin fish disease-resistant Chinese herbal medicine and yolk antibody compound additive, described compound additive is made up of three kinds of compositions of following weight ratio,

[0037] (1) Anti-Mandarin fish bacterial sepsis egg yolk antibody liquid 1;

[0038] (2) Three flavors of Chinese herbal medicine extract 3;

[0039] (3) β-cyclodextrin accounts for 8% of the total weight of the compound additive;

[0040] The preparation method of described anti-mandarin fish bacterial sepsis egg yolk antibody liquid is as follows:

[0041] ① The strong pathogenic pathogen Aeromonas hydrophila ( Aeromonas Hydrophila) G3 strains were inoculated on ordinary nutrient broth medium, cultured on a shaker at 30°C for 24 hours, added formaldehyde with a final volume concentration of 0.3% at 28°C for 48 hours, and centrifuged to obtain inactivated bacteria;

[0042] ② Soak Astragalus membranaceus in distilled water for 60 minutes, decoct, filte...

Embodiment 3

[0046] Embodiment 3, a kind of mandarin fish disease-resistant Chinese herbal medicine and egg yolk antibody compound additive, described compound additive is made up of three kinds of compositions of following weight ratio,

[0047] (1) Anti-Mandarin fish bacterial sepsis egg yolk antibody solution 1;

[0048] (2) Three flavors of Chinese herbal medicine extract 2;

[0049] (3) β-cyclodextrin accounts for 6% of the total weight of the compound additive;

[0050] The preparation method of described anti-mandarin fish bacterial sepsis egg yolk antibody liquid is as follows:

[0051] ① The strong pathogenic pathogen Aeromonas hydrophila ( Aeromonas Hydrophila ) G3 strains were inoculated on ordinary nutrient broth medium, cultured on a shaker at 29°C for 24 hours, added formaldehyde with a final volume concentration of 0.3% at 28°C for 48 hours, and centrifuged to obtain inactivated bacteria;

[0052] ② Soak Astragalus membranaceus in distilled water for 45 minutes, decoc...

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PUM

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Abstract

The invention relates to a disease-resistant Chinese herbal medicine and egg yolk antibody compound additive for siniperca chuatsi. The additive consists of egg yolk antibodies for resisting bacterialsepticemia of the siniperca chuatsi, lixivium of 3 Chinese herbal medicines and beta-cyclodextrin, wherein the egg yolk antibodies for resisting bacterial septicemia of the siniperca chuatsi are mainly used for reducing the possibility that the siniperca chuatsi is infected with high-pathogenicity aeromonas hydrophila or enabling the siniperca chuatsi to be prevented from being infected with thehigh-pathogenicity aeromonas hydrophila; in the lixivium of 3 Chinese herbal medicines, rheum officinale can restrain or destroy pathogenetic aeromonas hydrophila and has the effect of reducing the possibility that the siniperca chuatsi is infected with high-pathogenicity aeromonas hydrophila or enabling the siniperca chuatsi to be prevented from being infected with the high-pathogenicity aeromonas hydrophila, glossy privet fruits have the effect of enhancing immunity, and licorice root has the effect of removing toxicity; and the beta-cyclodextrin is mainly used for enabling a mixture of theegg yolk antibodies and the Chinese herbal medicine lixivium to have the effects of adhesion and slow release. The disease-resistant Chinese herbal medicine and egg yolk antibody compound additive disclosed by the invention can prevent and treat septicaemia caused by the pathogenetic aeromonas hydrophila during breeding of the siniperca chuatsi, the breeding yield of the siniperca chuatsi can be increased, and the breeding economic benefits of the siniperca chuatsi can be markedly increased. The problem that the bacterial septicemia is easy to generate during the breeding and producing courseof the siniperca chuatsi at present can be solved.

Description

technical field [0001] The invention relates to a feed additive for aquaculture, in particular to a feed additive for resisting mandarin fish bacterial sepsis. Background technique [0002] cocked mandarin fish ( Siniperca chuatsi ) is a ferocious freshwater carnivorous fish with a fast growth rate. It is very popular because of its tender meat and high nutritional content. At present, mandarin fish farming has become a major feature of my country's aquaculture industry. With the increase of intensive farming, the increase of breeding density and the deterioration of aquaculture water environment, the disease problem of mandarin fish is becoming more and more prominent, which has become the main factor threatening the healthy development of mandarin fish aquaculture in my country. Among these diseases, diseases such as bacterial sepsis caused by Aeromonas hydrophila are more serious, bringing huge economic losses to farmers and seriously restricting the healthy and stable d...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/30A23K10/18A01K61/13A61K39/395A61K47/40A61P31/04A61K36/708
CPCA61K36/484A61K36/638A61K36/708A61K39/395A61K47/40A23K10/18A23K10/30A23K50/80A01K61/13A61P31/04A61K2300/00Y02A40/81Y02A40/818
Inventor 高晓建张晓君刘晓丹李席席杨辉陈楠
Owner YANGZHOU UNIV
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