A kind of construction method of grass carp anti-bacterial sepsis strain

Abstract

The invention relates to a construction method of a grass carp anti-bacterial septicemia strain. The construction method comprises the following steps: a) selecting parent fishes, applying PIT (Point In Time) electronic tags to the parent fishes, when the parent fishes are mature in sexual glands, injecting hormone to accelerate maturation, performing natural fertilization, hatching fertilized ova to be baby fishes, cultivating fingerlings, and cultivating one-year-old fishes and two-year-old fishes in the same pool; b) respectively using pathogenic bacteria to infect offspring artificially in the baby fish phase and the 2-year-old fish phase, observing for 19-23 days after infection, and taking the residual individuals as a disease-resistant colony; c) adopting a paternity test method to perform progeny test on the obtained disease-resistant colony, selecting the parent fishes with strong disease resistance from the parent fishes, and in the second year, matching and propagating the parent fishes with strong disease resistance, and constructing the grass carp anti-bacterial septicemia strain. The construction method disclosed by the invention avoids the environment effect, can ensure that the obtained disease-resistant parent through screening is the real one with excellent heredity, ensures that the cultivated disease-resistant strain is of good disease-resistant performance; meanwhile, the method is easier to operate, and suitable for large-scale breeding for disease resistance.

Description

technical field [0001] The invention relates to the field of aquatic genetics and breeding, in particular to a method for constructing a bacterial sepsis-resistant strain of grass carp. Background technique [0002] Grass carp is a herbivorous fish, and it is also the main target of my country's excellent traditional freshwater fishery and freshwater aquaculture. Grass carp aquaculture has a history of more than 1,700 years in my country. In 2012, the output of grass carp in my country was more than 4.44 million tons, accounting for 20% of the total output of freshwater aquaculture fish in my country (Fishery Statistical Yearbook, 2012), ranking first in the output of freshwater aquaculture fish in the world. However, grass carp is vulnerable to diseases, including parasites, bacteria, viruses, etc., which bring huge economic losses to the grass carp aquaculture industry every year. [0003] Bacterial sepsis is also known as bacterial hemorrhagic disease and fulminant hemor...

AI Technical Summary

Problems solved by technology

However, although this patent document discloses a breeding method suitable for a new strain of fish with a relatively long sexual maturity time, it only discloses a technical route, and does not disclose a breeding method for a specific new strain of fish

On the other hand, the inventor of this patent application believes that the environmental conditions in the strain breeding process have a greater impact on the breeding effect and cannot be ignored. The families are separately cultivated in different environments until they reach 40-50 grams. The injection of PIT electronic markers for breeding in the same pond to select for growth traits does not ensure that the dominant family obtained is a family with excellent genetics, and the source of the parents determined later is not necessarily the parent with the best genes.

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