Vickers and aeromonas hydrophila bicombinant inactivated vaccine and large-scale preparation technology
A technology of Aeromonas hydrophila and dual inactivated vaccines, applied in vaccines, multivalent vaccines, veterinary vaccines, etc., can solve the problems of lack of large-scale preparation methods of vaccines, increase fishermen's income, and reduce water pollution , the effect of improving the effect of immunity
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[0015] The technical solutions of the present invention will be described in detail below through specific examples, but the scope of the present invention is not limited by these examples.
[0016] This embodiment provides a large-scale preparation technology of Vickers and Aeromonas hydrophila dual inactivated vaccine, including the following steps:
[0017] 1. Preparation of primary seed solution
[0018] Take out the strains (Aeromonas victorii and Aeromonas hydrophila) stored in the refrigerator, inoculate them on nutrient agar (see the appendix for the medium formula), cultivate them at 28°C for 24 hours, pick the moss and inoculate the nutrient agar at 28°C Cultivate for 24 hours, inoculate 300ml / bottle of nutrient broth (see the appendix for the medium formula), 10 bottles, place on a shaker at 200r / min, 28°C, and cultivate for 18 hours to obtain 3L of first-class seed liquid.
[0019] The strains selected in this example are: Aeromonas verkisii strain LYK090702 isola...
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