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A kind of grass carp bacterial septicemia and red skin disease combined vaccine and preparation method thereof

A bacterial and sepsis technology, applied in the field of grass carp bacterial sepsis and red skin disease combined vaccine and its preparation, can solve problems such as inability to achieve satisfactory results, achieve control of grass carp bacterial sepsis and red skin disease, reduce antibiotics and chemical The use of drugs, the effect of ensuring food safety and water ecological safety

Active Publication Date: 2022-06-21
广州普麟生物制品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For the prevention and control of grass carp bacterial diseases, tissue plasma vaccines or single bacterin immunization methods are mostly used, but none of them can achieve satisfactory results

Method used

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  • A kind of grass carp bacterial septicemia and red skin disease combined vaccine and preparation method thereof
  • A kind of grass carp bacterial septicemia and red skin disease combined vaccine and preparation method thereof
  • A kind of grass carp bacterial septicemia and red skin disease combined vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation of vaccine

[0024] (1) Aeromonas hydrophila (strain GA201) and Pseudomonas aeruginosa (strain JP802) stored at -70°C were inoculated with nutrient agar medium, incubated at 28°C for 24 hours, and single colonies were picked for inoculation. In nutrient broth medium, cultured at 28°C overnight (18h).

[0025] (2) Absorb 100 μL each of the Aeromonas hydrophila and Pseudomonas aeruginosa bacterial solutions cultured overnight in step (1), and inoculate them in the nutrient broth medium again, 28° C., 180 rpm, shaker Shake for 18 to 20 hours to expand the culture, and the concentration of Aeromonas hydrophila in the bacterial solution determined by McFarland turbidimetry was 7.5 × 10 8 CFU mL -1 , Pseudomonas aeruginosa was 4.2×10 8 CFU mL -1 .

[0026] (3) β-propiolactone with a final concentration of 0.1% (V / V) was added to the expanded Aeromonas hydrophila solution and Pseudomonas aeruginosa solution respectively, inactivated at 4°C for 72h, a...

Embodiment 2

[0029] Example 2 Preparation of vaccine

[0030] (1) Aeromonas hydrophila (strain GA201) and Pseudomonas aeruginosa (strain JP802) stored at -70°C were inoculated with nutrient agar medium, incubated at 28°C for 24 hours, and single colonies were picked for inoculation. In nutrient broth medium, cultured at 28°C overnight (24h).

[0031] (2) 90 μL of Aeromonas hydrophila and Pseudomonas aeruginosa bacteria cultured overnight in step (1) were sucked, respectively inoculated into nutrient broth medium again, 28° C., 180 rpm, shaker Shake for 18 to 20 hours to expand the culture, and the concentration of Aeromonas hydrophila was determined by McFarland turbidimetry to be 9 × 10. 8 CFU mL -1 , 6 × 10 for Pseudomonas aeruginosa 8 CFU mL -1 .

[0032] (3) β-propiolactone with a final concentration of 0.5% (V / V) was added to the expanded Aeromonas hydrophila solution and Pseudomonas aeruginosa solution respectively, inactivated at 5°C for 80h, and then heated at 40°C for 80 hour...

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Abstract

The invention discloses a grass carp bacterial septicemia and red skin disease combined vaccine and a preparation method thereof, using β-propiolactone as an inactivator to develop safe and efficient grass carp Aeromonas hydrophila septicemia and Pseudomonas aeruginosa Bacterial erythroderma dual white oil inactivated vaccine can effectively control the occurrence of bacterial septicemia and erythroderma in grass carp. After immunizing grass carp, it can stimulate the expression of IgM, IL-1β, IFN, Mx, C3 and TLR3 immune-related genes in the spleen and head kidney tissue of the fish, and the vaccine can produce a relative immune protection rate of 80-90% for grass carp , the relative protection rate can still maintain 50% 1 year after immunization; the invention is easy to operate, safe and effective, and two diseases can be prevented by one immunization, which not only reduces the use of antibiotics and chemical drugs, but also reduces the impact on the breeding environment and ecological environment, etc. The pollution and destruction of the external environment ensure the food safety and water ecological safety of grass carp.

Description

technical field [0001] The invention belongs to the field of fish farming, and particularly relates to a combined vaccine for bacterial septicemia and red skin disease of grass carp and a preparation method thereof. Background technique [0002] Grass carp is one of the main aquaculture species in Guangdong Province, with an annual output of more than 4 million tons, but the annual loss due to diseases is as high as more than 2 billion yuan. Grass carp septicemia and red skin disease are extremely serious bacterial diseases that harm grass carp, with a wide range of prevalence and high mortality. Grass carp bacterial sepsis is caused by the infection of Aeromonas hydrophila, which is a high incidence and high risk disease of grass carp in recent years. The disease season is long and the epidemic range is wide. The main grass carp breeding areas in the country all have the disease to varying degrees. Grass carp red skin disease has long plagued the majority of grass carp fa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/106A61K39/104A61P31/04
CPCA61K39/104A61K39/107A61P31/04A61K2039/70A61K2039/55511A61K2039/55A61K2039/521
Inventor 罗霞黄志斌江小燕刘春花陈总会赵长臣孙承文巩华陶家发
Owner 广州普麟生物制品有限公司
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