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Application of LECT2 protein and LECT2 protein variant in pharmacy

A technology of LECT2 and protein variants, applied in the application field of LECT2 protein, can solve the problems that LECT2 has not been found to affect the prevention and treatment of bacterial sepsis, and achieve the effects of preventing and treating sepsis, improving phagocytosis, and enhancing phagocytosis

Inactive Publication Date: 2012-07-11
NINGBO UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of LECT2 on the activity of macrophages and the prevention and treatment of bacterial sepsis have not yet been found.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Each milliliter of macrophages was treated with 5 μg of LECT2 protein or LECT2 protein variant (at least 60% amino acid sequence is identical to the amino acid sequence of LECT2 protein amino acid sequence) for 24 h, and the control was treated with the same dose of physiological water, and then treated with fluorescent balls (U.S. Molecular Probes Company) The phagocytosis of macrophages was observed with a fluorescence microscope. The results showed that the average macrophage phagocytosis of fluorescent microspheres in the control example treated with physiological water was 4.2 per cell, and each macrophage treated with LECT2 protein The average cell phagocytosis of fluorescent microspheres was 8.7 / cell, and the average phagocytosis of fluorescent microspheres per macrophage treated with LECT2 protein variants was 7.9 / cell; indicating that treatment with LECT2 or LECT2 protein variants can significantly improve macrophage The number, that is, the phagocytic ability o...

Embodiment 2

[0014] Intraperitoneal injection of 10 8 Escherichia coli in CFU / g body weight ( E. coli ) or 10 6 CFU / g body weight Pseudomonas aeruginosa ( P. Aeruginosa .) to establish a mouse sepsis model, the two pathogenic bacteria can cause more than half of the mice to die of sepsis within 48 hours at the above injection concentration. 30 minutes after the injection of Escherichia coli pathogenic bacteria, intraperitoneal injection of LECT2 protein (injection volume 0.5 μg / g body weight), 30 minutes after the injection of Pseudomonas aeruginosa, intraperitoneal injection of LECT2 protein variant (more than 60% of the amino acid sequence of the LECT2 protein The amino acid sequence is the same, the injection volume is 0.5 μg / g body weight), and a blank control (same dose of normal saline) is set at the same time. Comparing the survival rate of 48 h, 96 h and the final mouse after injection, the survival rate of blank control mice infected with Escherichia coli was about 43% at 4...

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Abstract

The invention discloses application of LECT2 protein and LECT2 protein variant in pharmacy, and particularly relates to application LECT2 protein and LECT2 protein variant in preparation of medicines for improving the phagocytosis of macrophage and / or preventing and treating bacterial septicemia. The LECT2 protein variant is a modification product of the LECT2 protein sequence, and is obtained by changing, replacing, substituting, inserting or losing one or more amino acids in the code sequence; at least 60% of the amino acid sequence of the LECT2 protein variant is the same as the amino acid sequence of the LECT2 protein; the LECT2 protein and LECT2 protein variant can induce the activity change of the macrophage to improve the phagocytosis of macrophage, and thus the septicemia caused by bacteria invasion can be prevented and treated; and the curate rate of a mice bacterial septicemia model can exceed 40%.

Description

technical field [0001] The invention relates to the application of LECT2 protein, in particular to the application of LECT2 protein and LECT2 protein variants in pharmacy. Background technique [0002] Leukocyte cell-derived chemotaxin 2 (LECT2 protein) is a multifunctional protein with a molecular weight of about 16 kDa and three intramolecular disulfide bonds. The molecular structure is simple and without glycosyl modification. LECT2 was initially considered as a neutrophil chemoattractant. With the deepening of research, it was found that it was also associated with bone and blood vessel growth, liver transplantation injury repair, plant toxins, non-pathogenic antigens, etc. Process-related, such as publication number CN102159238, titled composition for inhibiting pathological angiogenesis, which discloses that an effective amount of LECT2 protein or its analog (LECT2 protein variant) is prepared to inhibit pathological angiogenesis and / or or a composition of a cell prol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/19A61P37/04A61P31/04
Inventor 陈炯陆新江史雨红章瑞程吕技能虞朝晖裘芳
Owner NINGBO UNIV
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