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Fungus bacterial culture medium

A technology of bacterial culture medium and culture medium, applied in the field of microorganisms, can solve the problems of not being a culture medium, missed inspection, unsuitable for bacterial growth, etc., and achieve the effects of improving safety, increasing reliability and low cost

Inactive Publication Date: 2004-04-14
NAT INST FOR THE CONTROL OF PHARMA & BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese Pharmacopoeia medium is a suitable medium for the growth of fungi, but it is not suitable for the growth of bacteria. Even if the test product is contaminated with bacteria, there may be missed detection.
In the sterility test, half of the quantitative test product needs to be inoculated into the aerobic bacteria anaerobic medium to check the bacteria, and the other half is inoculated into the fungal medium to check the fungus. Suboptimal media for sterility testing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: preparation culture medium of the present invention

[0016] Pour 1000 ml of distilled water into a 2000 ml glass container, weigh 13 grams of peptone, 4 grams of yeast extract powder, 20 grams of glucose, 4 grams of sodium chloride, 4 grams of dipotassium hydrogen phosphate and 0.7 grams of magnesium sulfate heptahydrate and place them in the glass In the container, shake evenly. The pH of the medium thus obtained was 7.4.

Embodiment 2

[0017] Embodiment 2: preparation culture medium of the present invention

[0018] Pour 1000 ml of distilled water into a 2000 ml glass container, weigh 15 grams of peptone, 3 grams of yeast extract powder, 15 grams of glucose, 5 grams of sodium chloride, 3 grams of dipotassium hydrogen phosphate and 0.5 grams of magnesium sulfate heptahydrate and place them in the glass In the container, shake evenly. The pH of the medium thus obtained was 7.2

Embodiment 3

[0019] Embodiment 3: preparation culture medium of the present invention

[0020] Pour 1000 ml of distilled water into a 2000 ml glass container, weigh 17 grams of peptone, 2 grams of yeast extract powder, 8 grams of glucose, 6 grams of sodium chloride, 2 grams of dipotassium hydrogen phosphate and 0.3 grams of magnesium sulfate heptahydrate and place them in the glass In the container, shake evenly. The pH of the medium thus obtained was 7.0

[0021] Effect example: the spirit of Chinese Pharmacopoeia culture medium, United States Pharmacopoeia culture medium and culture medium of the present invention sensitivity comparison

[0022] Three batches of newly-produced dried Chinese Pharmacopoeia medium and three batches of USP medium were used as the culture medium. The medium was provided by the China Institute for the Control of Pharmaceutical and Biological Products. The batch numbers are: 1109, 001108, 00032. The culture medium of the present invention adopts the cu...

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PUM

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Abstract

The present invention relates to a sterility test speical-purpose culture medium suitable for growth of fungi and for growth of bacteria. Said culture medium is formed from peptone, yeast extract powder, glucose, sodium chloride, potassium phosphate dibasic, bitter salt and water.

Description

(1) Technical field [0001] The invention belongs to the field of microorganisms. More specifically, the present invention relates to a special culture medium for sterility test which is suitable for both fungal growth and bacterial growth. (2) Background technology [0002] As we all know, the culture medium is the substrate for the artificial synthesis of various nutrients for the growth of microorganisms. Generally speaking, it is necessary to adopt different media for cultivating microorganisms of different nutritional types. For example, there are some special media in the sterility test. The Chinese Pharmacopoeia 2000 edition contains a kind of fungal culture medium for sterility testing (Chinese Pharmacopoeia, 2000 edition (Part Two) Appendix 89, medium 2, hereinafter referred to as "Chinese Pharmacopoeia medium"), USP 24 edition pharmacopoeia contains a soybean-tryptone medium for sterility testing (USP 24:1818, hereinafter referred to as "USP Medium"). The USP med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20
Inventor 苏德模许华玉刘鹏
Owner NAT INST FOR THE CONTROL OF PHARMA & BIOLOGICAL PROD
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