A method for the controlled cultivation of cell density and arrangement based on insect wing substrate

Abstract

The invention discloses a controllable culture method of cell density and arrangement based on an insect wing substrate. The method uses insect scale wings as a base, and the insect scale wings are placed obliquely in a cell suspension, and the cells fall vertically under the action of gravity. On the surface of the insect scales, the number of cells gradually increases from top to bottom; the cells on the surface of the insect scales are oriented according to the micro-nano structure on the surface of the insect scales. The invention uses insect scales as the substrate, which is easy to obtain, safe and non-toxic, and can realize cell culture with controllable cell density and arrangement at the same time, and avoids a series of complex processes and safety problems caused by artificial synthetic materials; it uses gravity to form The density gradient distribution avoids the damage of mechanical force to cells and retains cell viability. The formed cell arrangement with directional arrangement and density gradient distribution has potential application prospects and reality for tissue regeneration, including regeneration and repair of connective tissue. significance.

Description

technical field [0001] The invention belongs to the technical field of biological materials and tissue engineering, and in particular relates to a controllable culture method based on the cell density and arrangement of insect wing substrates. Background technique [0002] With the development of organ and tissue repair, transplantation, and biosensor research in tissue engineering, the directional arrangement and growth of cells and the gradient distribution of cell density are becoming more and more important. At present, with the maturity of micro-nano technology, the substrate materials for cell culture can achieve controllable cell adhesion, directional arrangement and other behaviors in vitro. At the same time, the gradient distribution of cell density can also be achieved in vitro by using mechanical force, physical and chemical methods, and inkjet printer technology. [0003] However, the synthesis process of many base materials is complicated, and even involves som...

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Problems solved by technology

[0003] However, the synthesis process of many substrate materials is complicated at present, and even involves some toxic substances, and both physical and chemical methods and printing techniques will cause damage to cell viability

In addition, in the current research on cell culture, there is almost no method that can achieve a cell culture method with simultaneous controllable cell density and arrangement.

Therefore, it cannot meet the needs of fields such as medical implants, tissue engineering, especially bone joint tissue engineering.

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