Production process for potato bacterial wilt resistant compound microbial agent
A compound microbial agent, potato bacterial wilt technology, applied in the direction of microorganism-based methods, microorganisms, biocides, etc., can solve the problems of inability to achieve long-term disease resistance, ineffective effect, waste of traditional Chinese medicine resources, etc., and achieve enhanced The effect of plant stress resistance, simple and easy production process, and high survival rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] a) Preparation of Bacillus colloidus cells: the strains were purchased from Beina Biology, strain number BNCC173119, 50 kg of culture medium was prepared, and the formula was 0.5 kg of peptone, 0.15 kg of beef extract, 0.25 kg of sodium chloride, pH 7.5, 121 Sterilize at ℃ for 30 minutes, insert the purchased strains, and culture on a shaking table at 150r / min for 24 hours to obtain the strains; prepare 1t of expansion medium, the formula is 40kg of beef extract, 9kg of yeast extract powder, 3kg of sodium chloride, pH7.5 , transferred to a fermenter for high-temperature sterilization, and after the sterilization, when the temperature dropped to 36°C, 50kg of the seed liquid was inserted, and the rotation speed was 120r / min for 26h, and the fermentation liquid was transferred to 4000r / min and centrifuged for 30min to obtain Bacillus colloidus Bacteria 128.7kg.
[0026] b) Preparation of Trichoderma harzianum thallus: purchased from Trichoderma Strain Collection Managemen...
experiment example 1
[0033] Experimental example 1: Potted plant disease control experiment of potato bacterial wilt
[0034] Use the compound microbial inoculum produced by the method of embodiment 1 to carry out potted plant disease prevention test, test procedure is as follows:
[0035] The experimental potato variety "Holland No. 7" was purchased locally. The bacterial wilt pathogen, R. solanacearum, was purchased from Beina Biological No. BNCC221120. The experimental soil was taken from the field of Youyi Village, Laishan Town, Yantai City, which is prone to potato bacterial wilt. Soil, texture sandy loam, pH=5.3, organic matter 7.3g / kg, total nitrogen (N) 0.762g / kg, total phosphorus (P 2 o 5 )0.577g / kg, total potassium (K 2 O) 15.3g / kg. A total of 3 treatments were set up in the test, which are: T1: root irrigation with Jinzhengda antimicrobial agent, the application rate is 3ml / m 2 ; T2: use the bacterial agent of the present invention to spread, and the application rate is 5g / m 2 ; T3...
experiment example 2
[0040] Experimental example 2: Field experiment on the control of potato bacterial wilt
[0041] Use the compound microbial bacterial agent produced by the method of embodiment 1 to carry out field test, from April 2016 to September 2016, carry out field test in Xiaohezi Village, Fushan District, Yantai City, and the experimental field for testing is the incidence rate of bacterial wilt disease in recent years. The more serious plots were spread with organic fertilizers, and the experimental potato variety was Yanshu No.4. The test set 2 treatments, respectively: T1: use the bacterial agent of the present invention to spread, and the application rate is 5kg / 667m 2 ; T2: No bacterial agent was used as the control group. Each treatment was repeated 4 times, and the area of the plot was 100m 2 .
[0042] Observe the disease on 30d, 60d, 90d, and 120d after planting. Disease grade: 0 is asymptomatic on the leaves; 1 is wilting on the leaves below 1 / 4 of the plant; The surfac...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com