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Composite microbial agent and preparation method and application thereof

A technology of compound microbial inoculants and seeds, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of large environmental impact, less research on multi-functional compound spore inoculants, and single action mechanism of a single strain, etc. problems, to achieve the effect of long storage time, good application prospects, and significant nitrogen saving effect

Inactive Publication Date: 2017-09-19
DONGGUAN BAODE BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many reports on the use of a single microorganism to prevent and control plant diseases and promote plant growth, but a single strain has a single mechanism of action and is greatly affected by the environment.
The combination of growth-promoting bacteria can realize the complementary advantages and synergy among multiple strains, enhance their adaptability, competitiveness and survival rate in the natural environment, and give full play to their growth-promoting and bio-control effects. Less research has been done on spore-forming agents

Method used

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  • Composite microbial agent and preparation method and application thereof
  • Composite microbial agent and preparation method and application thereof
  • Composite microbial agent and preparation method and application thereof

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Effect test

Embodiment 1

[0045] The preparation method of the composite microbial bacterial agent of the present embodiment, it may further comprise the steps:

[0046] 1), seed cultivation:

[0047] Prepare seed medium A and seed medium B first, then inoculate 1 loop of Paenibacillus nitrogen-fixing species 1-49 into seed medium A with an inoculation loop, and inoculate 1 loop of Bacillus megaterium B16 and 1 loop of Paenibacillus megaterium B16 and 1 loop solution separately with the inoculation loop Pour Bacillus amylogenus T600 into two seed culture medium B with a rotating speed of 180r / min and a temperature of 30°C for 24 hours on a shaker to expand the cultivation and activation to obtain Paenibacillus 1-49 seed liquid, giant Bacillus B16 seed liquid and Bacillus amyloliquefaciens T600 seed liquid;

[0048] 2), fermentation culture:

[0049] Prepare the fermentation medium first, then take 10ml of the Paenibacillus ring-fixing 1-49 seed solution, 20ml of the Bacillus megaterium B16 seed solut...

Embodiment 2

[0054] The preparation method of the composite microbial bacterial agent of the present embodiment, it may further comprise the steps:

[0055] 1), seed cultivation:

[0056] Prepare seed medium A and seed medium B first, then inoculate 1 loop of Paenibacillus nitrogen-fixing species 1-49 into seed medium A with an inoculation loop, and inoculate 1 loop of Bacillus megaterium B16 and 1 loop of Paenibacillus megaterium B16 and 1 loop solution separately with the inoculation loop Bacillus amylogenus T600 was put into two seed culture medium B, the rotation speed was 200r / min, the temperature was 32°C, and the shaker culture was carried out for 24 hours, and the expanded culture and activation were carried out to prepare the seed liquid of Paenibacillus azotrophus 1-49, giant Bacillus B16 seed liquid and Bacillus amyloliquefaciens T600 seed liquid;

[0057] 2), fermentation culture:

[0058] Prepare the fermentation medium first, then take 10ml of the Paenibacillus ringfixing 1...

Embodiment 3

[0063] The preparation method of the composite microbial bacterial agent of the present embodiment, it may further comprise the steps:

[0064] 1), seed cultivation:

[0065] Prepare seed medium A and seed medium B first, then inoculate 1 loop of Paenibacillus nitrogen-fixing species 1-49 into seed medium A with an inoculation loop, and inoculate 1 loop of Bacillus megaterium B16 and 1 loop of Paenibacillus megaterium B16 and 1 loop solution separately with the inoculation loop Pour Bacillus amylogenus T600 into two seed culture medium B with a rotating speed of 220r / min and a temperature of 4°C for 20 hours on a shaking table to expand the cultivation and activation to prepare Paenibacillus 1-49 seed liquid, giant Bacillus B16 seed liquid and Bacillus amyloliquefaciens T600 seed liquid;

[0066] 2), fermentation culture:

[0067] Prepare the fermentation medium first, then take 10ml of the Paenibacillus ring-fixing 1-49 seed solution, 20ml of the Bacillus megaterium B16 see...

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Abstract

The invention relates to the technical field of microbial inoculations, in particular to a composite microbial inoculation and its preparation method and application. The compound microbial agent includes Paenibacillus nitrogen-fixing 1-49, Bacillus megaterium B16 and Bacillus amyloliquefaciens T600; the preparation method of the compound microbial agent includes seed culture, fermentation culture, and the optimal combination of different bacterial liquid additions in the fermentation culture is passed through positive The cross-test confirmed; the compound microbial agent is applied to promote the growth of sugarcane and improve the agricultural nitrogen-saving effect. The composite microbial bacterial agent of the present invention has a remarkable nitrogen-saving effect and has a huge market advantage; the present invention optimizes the addition amount of different bacterial solutions for liquid fermentation through orthogonal experiments, improves the spore production of nitrogen-fixing bacteria, and greatly improves the quality of production , can effectively reduce production costs; the three strains of the present invention are spores, which have the advantages of long storage time and long shelf life, and will have good application prospects in future agricultural production.

Description

technical field [0001] The invention relates to the technical field of microbial inoculations, in particular to a composite microbial inoculation and its preparation method and application. Background technique [0002] For a long time, my country's agricultural production has mainly relied on the input of a large amount of chemical fertilizers and pesticides. The agricultural ecological environment problems caused by the unreasonable use of chemical fertilizers and pesticides have become increasingly serious. In recent years, with the enhancement of people's awareness of environmental protection and the increasing emphasis on pollution-free agricultural products, the research and development of new technologies and new products has continued to deepen, and the research on exploring new sources of biological fertilizers and biological control to replace or partially replace chemical fertilizers and pesticides has become more frequent. attention. Therefore, the application o...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C12R1/11C12R1/07C12R1/01
CPCC12N1/20C05B7/00C05G3/00
Inventor 尹坤尹涛孙旭生杨国平刘剑敏王亚君杨盼盼李艳林敏沈世华陈三凤张学贤
Owner DONGGUAN BAODE BIOLOGICAL ENG
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