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Strain with high cellulose enzyme activity and application and obtaining methods thereof

A technology with high cellulose and enzymatic activity, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low cellulase degradation efficiency, high cellulose conversion cost, and large amount of cellulase. , to reduce the cost of cellulose conversion, improve the conversion efficiency, and achieve the effect of high cellulase activity

Active Publication Date: 2015-06-24
HUNAN LONGTENG BIOTECH
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AI Technical Summary

Problems solved by technology

[0004] One of the key factors restricting the utilization of cellulosic materials is the low efficiency of cellulase degradation and the large amount of cellulase used, resulting in high cost of cellulose conversion

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Preliminary screening

[0019] Pulp sludge was collected from the papermaking waste yard as a sample, and 1 g of the sample was dissolved in phosphate buffered saline (PBS), vortexed and shaken, and left to stand for 30 minutes to allow the residue to sink, and the supernatant was diluted 100 times, and coated with The cloth was inoculated on the agar plate growth medium R2A (0.5g / L yeast extract, 0.5g / L peptone, 0.5g / L casein, 0.5g / L glucose, 0.5g / L soluble starch, 0.3g / L hydrogen phosphate dipotassium, 0.5g / L magnesium sulfate heptahydrate, 0.3g / L sodium pyruvate, 15.0g / L agarose). Cultivate at 28°C. After culturing for 24, 48, and 72 hours respectively, observe the diameter, shape, and color of the single colony growing on the plate. Select colonies with medium diameter, neat edges, sunken middle, and off-white color. The obtained colonies were repeatedly streaked on plates to obtain purified strains for further screening.

[0020] In another embodiment of the ...

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PUM

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Abstract

The invention discloses a strain with high cellulose enzyme activity and application and obtaining methods thereof. The strain is named as paennibacillus (Panenibacillus sp.WH1) which is stored at a typical culture preservation centre in China in Luojia Hill, Wuchang, Wuhan City, Hubei Province, the serial number of registration of preservation is CCTCC No: M2014236, and preservation data is May 29, 2014. The paenibacillus sp WH1 with high cellulose enzyme activity is obtained by being separated from pulp sludge, so that higher cellulose enzyme activity is achieved; the paenibacillus sp WH1 can be widely used in food industry, brewing industry, paper industry, feed industry, textile industry and biomass conversion, biomass material conversion efficiency can be improved, and cellulose conversion cost can be lowered.

Description

technical field [0001] The invention relates to an engineering strain with cellulase, in particular to a strain with high cellulase activity and its application and obtaining method. Background technique [0002] Cellulase is a general term for a group of enzymes that degrade cellulose to produce glucose, mainly including endoglucanase, exoglucanase and β-glucosidase. The three components of cellulase act synergistically to degrade macromolecular cellulose into low molecular weight and low oligosaccharides, disaccharides or monosaccharides. Cellulase is widely used in food industry, brewing industry, paper industry, feed industry, textile industry and biomass conversion. [0003] Cellulose is one of the most abundant renewable resources in nature. Cellulase can be used to degrade natural cellulose into usable sugars, and then convert them into bioalcohol or other usable biomolecular materials through fermentation technology, so as to realize the efficient utilization of bi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20C12N1/205C12R2001/01
Inventor 唐建
Owner HUNAN LONGTENG BIOTECH
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