Microbial preparation as well as preparation method and application of microbial preparation in preventing and treating plant diseases
A technology for microbial preparations and strains, applied in the field of microbial inoculants, can solve problems such as affecting the efficacy of microbial fertilizers, unreasonable formulations, and lack of fermentation metabolites, so as to optimize soil nutrients, adjust soil microecological composition, and improve biological activity. Effect
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Embodiment 1
[0061] Jelly-like bacillus 0.1kg, shrimp powder 10kg, collagen 0.01kg, NaCl 9kg, amino acid 1kg, carbohydrate 20kg, compound fertilizer 1kg.
[0062] Among them, collagen is fish skin collagen; amino acid is complex amino acid extracted from soybean protein, and 20 g of zinc sulfate and 5 g of boric acid are added for every 100 g of amino acid; carbohydrate is sucrose, and N / P / K=15 / 15 / 15 in compound fertilizer.
[0063] 1000L of water, adding strains, shrimp shell powder, collagen, NaCl bubbling and aeration for cultivation, the cultivation temperature was kept at 30°C, and the cultivation time was 3 days;
[0064] On the basis of one culture, add amino acid, carbohydrate, and compound fertilizer, continue bubbling and ventilating for culture, keep the culture temperature at 30°C, and culture for 4 days to obtain the microbial preparation.
Embodiment 2
[0066] Jelly-like bacillus 1kg, crab powder 0.1kg, collagen 1kg, NaCl 0.01kg, amino acid 50kg, carbohydrate 1kg, compound fertilizer 10kg.
[0067] Among them, the collagen is pigskin collagen, the amino acid is a compound amino acid extracted from keratin, 40g of zinc sulfate and 20g of boric acid are added for every 100g of amino acid, the carbohydrate is fructose, and N / P / K=15 / 15 / 15 in the compound fertilizer.
[0068] 1000L of water, adding strains, crab powder, collagen, NaCl bubbling and aeration for cultivation, the cultivation temperature was kept at 28°C, and the cultivation time was 4 days;
[0069] On the basis of one culture, amino acids, carbohydrates, and compound fertilizers were added, and the culture was continued by bubbling and aeration. The culture temperature was kept at 32° C., and the culture time was 3 days to obtain the microbial preparation.
Embodiment 3
[0071] Bacillus subtilis 2.0kg, Bacillus jelly-like 0.5kg, Lysobacter colloidus 1.0kg, crab powder 0.5kg, shrimp powder 0.5kg, collagen 0.05kg, NaCl 0.05kg, amino acid 10kg, carbohydrate 20kg, compound fertilizer 10kg.
[0072] Among them, the collagen is fish skin collagen, the amino acid is vegetable leaf fermentation product, 40 g of zinc sulfate and 5 g of boric acid are added for every 100 g of amino acid, the carbohydrate is sucrose, and N / P / K=15 / 15 / 15 in the compound fertilizer.
[0073] 1000L of water, adding strains, shrimp shell powder, collagen, NaCl bubbling and aeration for cultivation, the cultivation temperature was kept at 30°C, and the cultivation time was 3 days;
[0074] On the basis of one culture, add amino acid, carbohydrate, and compound fertilizer, continue bubbling and ventilating for culture, keep the culture temperature at 30°C, and culture for 4 days to obtain the microbial preparation.
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