38 results about "Lysobacter" patented technology

The invention provides a biocontrol bacterial strain for preventing and treating plant diseases and its bacterial agent, belonging to the field of biological control. The strains used are Gram-negative bacteria, identified as Lysobacter enzymogenes, and the strain code is OH11. Strain OH11 has no flagella but has slippage, can produce various extracellular hydrolytic enzymes including chitinase, β-1,3-glucanase and protease, and can effectively inhibit the growth of fungi and bacteria. The antibacterial zone diameters of strain OH11 against Sclerotinia sclerotiorum and Phytophthora capsici were both greater than 22.0 mm; the antagonism against potato ring rot was stronger, and the diameter of the inhibition zone reached 50 mm. The OH11 strain was inoculated into the seed tank, cultivated to the logarithmic growth phase, and the seed liquid was connected to the production tank for cultivation. The medium used in the production tank was the same as that of the seed tank. The liquid fermentation adopts aerobic submerged fermentation and fed-batch process, the dissolved oxygen is 15%-20%, the fermentation temperature is 30°C, the fermentation time is 72h, and the initial pH value is 7.5. After the fermentation is completed, the culture solution is taken out of the tank and directly packed into liquid dosage forms with plastic packaging barrels or packaging bottles, or subpackaged into solid dosage forms with peat adsorption packaging bags. The biocontrol strain OH11 can effectively control plant pathogenic fungi, bacteria, nematodes and other diseases, and the overall control effect is 50%-70%. In the greenhouse pot experiment, the control effects of OH11 on pepper blight and tomato bacterial wilt reached 83.6% and 86.4%, respectively. Strain OH11 has the characteristics of broad antibacterial spectrum, high activity, and environmental safety. In today's serious pesticide pollution, zymolysobacterium and its bacterial agent will be a good substitute.

The invention provides Lysobacter capable of efficiently degrading aflatoxin B1 and ochratoxin A and application of the Lysobacter and particularly provides double-function Lysobacter sp. CW239 and application thereof to the degradation of low-pollution concentration aflatoxin B1 and ochratoxin A. Compared with existing aflatoxin degrading bacteria, the Lysobacter sp. CW239 has the advantages that the Lysobacter sp. CW239 can achieve excellent degrading effect under a low-concentration toxin pollution condition; under a liquid fermentation condition, in fermentation broth, which contains the aflatoxin B1 and ochratoxin A, with the final concentration of 20 microgram / L, the 12-hour ochratoxin A degradation rate of the Lysobacter sp. CW239 is 53.1%, and the 48-hour degradation rate reaches 99.8%; the 12-hour aflatoxin B1 degradation rate of the Lysobacter sp. CW239 is 42.5%, and the 48-hour degradation rate reaches 83.4%; when the Lysobacter sp. CW239 is used for processing feed (with the final concentration of 20 microgram / kg) polluted by toxins, the 48-hour ochratoxin A degradation rate is 68.7%, and the 48-hour aflatoxin B1 degradation rate is 52.1%; the Lysobacter sp. CW239 has substantial application value and significance when being applied to food and feed bio-detoxification.

The invention relates to a construction strategy and a construction process of an unmarked lysobacter enzymogenes engineering strain capable of preventing plant bacteriosis, and belongs to the field of microbial genetic engineering. An excellent exogenous gene aiiA is directionally integrated onto a chromosome, and any marked genes are not brought into the unmarked lysobacter enzymogenes engineering strain. A quorum-sensing system for plant pathogenic bacteria can be efficiently damaged by the engineering strain, pathogenicity of the pathogenic bacteria on a host plant (Chinese cabbage) is remarkably reduced, and biological prevention of the plant bacteriosis is realized.

The invention discloses a method for producing active antimicrobial substance HSAF by optimizing lysobacter enzymogenes OH11 with response surface method. The method comprises: strain activation, seed liquid culture, fermentation culture, and extraction and detection of HSAF in fermentation broth. A fermentation medium comprises following raw materials according to a ratio: 8.00g / L of soy flour, 7.89g / L of glucose and 0.72g / L of CaCl2. The liquid containing amount, the culture temperature and the fermentation period are 22%, 26 DEG C and 58h respectively. Inert substances are added into HSAF extraction process to eliminate an emulsion problem, so that the fermentation liquid HSAF can be accurately quantitative, and ultimately the yield of HSAF can be up to 440.26mg / L, which is 15 times that produced under conventional conditions (10% TSB, 29.24mg / L). The method has the advantages of being extensive in raw materials, low in cost, capable of effectively reducing the production cost of HSAF, and providing important technical parameters for the future production of HSAF.

The invention relates to a preparation method of a natural colorless biological mildew-proof anticorrosive bactericide. The preparation method comprises the following steps: firstly inoculating lysobacter enzymogenes C3 and a circle of seeds in a conical flask, and culturing on a shaking table to obtain a lysobacter enzymogenes C3 seed liquid; putting a liquid culture medium in a fermentation tank and then inoculating the lysobacter enzymogenes C3 seed liquid for culture to obtain a high-activity lysobacter enzymogenes C3 mildew-proof anticorrosive bactericide; heating the high-activity lysobacter enzymogenes C3 mildew-proof anticorrosive bactericide for 2-10 minutes at the temperature of 60 to 100 DEG C in order to perform thermal treatment; finally adding modified active carbon into the bactericide to perform a decoloration reaction to obtain the natural colorless biological mildew-proof anticorrosive bactericide, wherein the amount of the added modified active carbon is 0.5-3.0 percent of the weight of the high-activity lysobacter enzymogenes C3 mildew-proof anticorrosive bactericide which is in light brown. Due to acidic modification, an acidic oxygen-containing functional group on the surface of the active carbon is enabled to have polarity property, so the adsorption on compounds which are relatively high in polarity is enhanced, the adsorption capacity of the active carbon is improved, and the preferential adsorption of the active carbon on specific impurities like pigments is improved.

The invention relates to a biocontrol bacterium strain OH17 with good antagonistic activity for plant pathogenic fungi and oomycete. The bacterial strain is characterized in that: the bacterial strain is Lysobacter gummosus, which belongs to Lysobacter of Xanthomonadaceae, with CGMCC No. 8649. The form of the bacterium colony is as follows: the bacterium colony presents different forms on different mediums, for example, the bacterium colony is in light yellow color on a NA medium, the thallus surface is smooth with luster, and the surface is protruding; the bacterium colony is always in yellow color on an LB medium with slow growth; the bacterium colony is white on a 10% TSA medium with flat surface; the bacterium colony does not grow on any TSA medium. The bacterium is gram-negative and in a rod shape without any flagellum. The optimum growth temperature is 28 DEG C. The bacterial strain has a wide spectrum and high efficiency antagonistic action on plant pathogenic fungi and oomycete.

The invention provides 2,5-diketopiperazine dipeptide (I) derived from lysobacter enzymogenes as well as a preparation method and an application thereof and an endophytic bacterium producing 2,5-diketopiperazine dipeptide, namely lysobacter enzymogenes R-2-1. 2,5-diketopiperazine dipeptide (I) has the main beneficial effects that (1) lysobacter enzymogenes R-2-1 producing 2,5-diketopiperazine dipeptide (I) is provided and is the first lysobacter enzymogenes produced in the strain; (2) 2,5-diketopiperazine dipeptide (I) has strong inhabiting effects on aspergillus flavus and candida albicans, can serve as a novel bactericide and can be further used for preparing medicament for treating relevant diseases caused by aspergillus flavus or candida albicans; (3) 2,5-diketopiperazine dipeptide (I) is produced by liquid fermentation of lysobacter enzymogenes R-2-1 and has the advantages of simple and convenient operation process, short period, low cost and ensured origin; (4) 2,5-diketopiperazine dipeptide (I) is synthesized by a biological method and does not pollute the environment.

The invention discloses tobacco spice prepared by fermenting fructus holboelliae latifoliae by using microorganisms and application thereof. The fructus holboelliae latifoliae is used as a raw material; a lysobacter C8-1 is subjected to amplification culture and is used for fermenting the fructus holboelliae latifoliae; shaking culture at 30 DEG C is performed for 24 to 48h; then, through backflowextraction, filtering slag removal and supernate pressure reduction concentration, the tobacco spice is obtained. The preservation number of the bacterial strain is CGMCC 1.16271. Though microbiological characteristic analysis and 16S rRNA gene sequence analysis shows that the lysobacter is a microorganism new spice. Compared with a product without treatment in a control group, the product obtained by simultaneously fermenting the bacterial strain and the fructus holboelliae latifoliae has the advantages that the sweet and sour feeling of the cigarette is enhanced; the fluid promoting effectis obvious; the smoke gas is soft, fine and smooth; the fragrance richness is enhanced.

The invention discloses lysobacter sp and application thereof. The strain is named as lysobacter sp C8-1, is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 28, 2017, and has a preservation number of CGMCC No.1.16271. Microbiological characteristic analysis and 16S rRNA gene sequence analysis show that the strain is a new type of bacteria. The strain can produce benzaldehyde and volatile compounds such as geosmin, elemene, elemol and alpha-eudesmol, and can be used for preparing tobacco flavor after subjected to fermentation. The obtained flavor has very special aroma, thus being capable of enhancing the style of cigarettes, enriching cigarette fragrance, and enabling the aroma to be mellow and fuller; furthermore, the strain has antibacterial andfungal activity, and is stable and lasting in effect. In addition, the strain has great potential in development of novel, highly effective, non-toxic natural medicines.

The invention discloses an extracellular polysaccharide lysobacter SCSIO 17111 with sand fixation effect and application thereof. The lysobacter SCSIO17111 was collected into Common Microorganism Center of China Committee for Culture Collection of Microorganisms (CCCCM ) on August 18th, 2018; the address is Institute of Microbiology, Chinese Academy of Sciences at No.3, No.1 Community, Beichen West Road, Chaoyang District, Beijing City; the collection number is CGMCC NO.14537. The lysobacter SCSIO17111 has the advantages that the sand particles can be agglomerated and the relative stable stateis maintained, so as to reach the sand fixation effect, and apply to the prevention and control of desertification in arid and semi-arid areas and the construction of reef biological soil crust. Compared with the physical sand fixation, chemical sand fixation and vegetation cultivation methods, the lysobacter SCSIO17111 has the advantages that by using the microorganism crust sand fixation type as the novel sand fixation type, the adaptability is strong, the cost is low, the effect is quick, and the like.

The invention belongs to the technical field of seed coating agents, and relates to a biological seed coating agent and a preparation method thereof. The preservation information of a beneficial bacterial strain is CGMCC No.2403 2008.03.17. The biological seed coating agent comprises beneficial bacteria and an auxiliary agent, wherein the beneficial bacteria and the auxiliary agent are mixed in proportion; the beneficial bacteria are antibiotic lysobacter; the auxiliary agent comprises a film forming agent, a wetting agent, a dispersing agent, a thickening agent, a protective agent and warningcolor; the film forming agent is formed by mixing polyvinyl alcohol and chitosan; the wetting agent is OP-10; the dispersing agent is sodium lignosulfonate; the thickening agent is xanthan gum; the protective agent is glycerol; and the warning color is eosin. The preparation method comprises the following steps: uniformly mixing antibiotic lysobacter and the auxiliary agent according to a proportion to prepare the biological seed coating agent. The biological seed coating agent has no influence on plant growth safety, belongs to an environment-friendly preparation, and is suitable for the requirement of sustainable green agricultural development.

The invention provides composite agricultural microorganism bacteria and a method for applying composite bacteria to lotus root planting. All strains of compound bacteria comprise, by weight, 20% of rhodospirillum, 10% of nitrospira, 10% of azotobacter, 10% of bacilli, 10% of thermoactinomyces, 10% of xanthomonas, 10% of lysobacter, 10% of acidophilic bacteria and 10% of novosphingobium. The composite bacteria are used for lotus root planting, the yield of lotus roots is increased, and the quality of the lotus roots is improved.

The invention discloses a cigarette spice prepared by microbiological fermentation of prunella vulgaris and application of the cigarette spice. The cigarette spice is obtained by taking the prunella vulgaris as a raw material, performing amplification culture on lysobacter C8-1 to ferment the prunella vulgaris, performing shake culture for 24-48h at 30 DEG C, and then performing reflux extraction,slag removal by filtration and vacuum concentration of supernatant liquid. The preservation number of the strain is CGMCC 1.16271. Microbiological characteristic analysis and 16S rRNA gene sequence analysis show that the lysobacter is a new microbiological strain. Compared with untreated controls, the product obtained by co-fermentation of the lysobacter C8-1 and the prunella vulgaris has the advantages that under the condition of keeping the original characteristics and smell of the prunella vulgaris, the bitter taste is reduced, and the effect of promoting the secretion of saliva or body fluid is obvious.

The invention discloses a complex microbial agent capable of purifying air and application thereof. The complex microbial agent is prepared from, by weight, 7-15 parts of pseudomonas, 7-15 parts of saccharomycetes, 6-14 parts of Opitutales, 5-12 parts of rhizobium, 4-12 parts of rhodospirillum, 4-12 parts of rhizopus oryzae, 2-9 parts of micrococcus, 2-8 parts of bacteroides, 2-9 parts of clostridia, 2-8 parts of bacillus cereus, 1-7 parts of alcaligenes, 1-7 parts of streptomycete, 1-7 parts of rhodocyclus, 1-6 parts of galactococcus, 1-6 parts of mycobacteria, 1-6 parts of burkholderiales, 1-6 parts of lysobacter, 1-5 parts of hydrogenophilus, 1-5 parts of xanthomonas, 1-5 parts of brevundimonas, 0.1-3 parts of comamonas and 0.1-3 parts of aspergillus. The complex microbial agent utilizes the synergistic effect of powerful bacteria, it is promoted that microorganisms utilize and perform metabolism decomposition on volatile organic compounds in the air, so that the efficient elimination and reduction of contaminants are achieved.

The invention provides a lysobacter enzymogenes mutant strain TGJZC-041. The method comprises the following steps: inoculating the bacteria into a nutrient agar culture-medium slope; standing and culturing at 30+ / -1 DEG C for 2-3 days; inoculating into the fermentation medium at the inoculum size of 5-10%; carrying out shake culturing in a constant temperature shaker at 30+ / -1 DEG C at the rotating speed of 215-225rpm for 3 days, so as to prepare an incision enzyme for lysine peptide. The lysobacter enzymogenes mutant strain has the beneficial effects that the Lys-C generated by the mutant strain is relatively high in expression quantity and shorter in fermentation period.

By culturing Lysobacter sp. BMK333-48F3 (deposit number of FERM BP-7477), an antibiotic, tripropeptin Z, tripropeptin A, tripropeptin B, tripropeptin C or tripropeptin D represented by the general formula (I):wherein R is 7-methyl-octyl group, 8-methyl-nonyl group, 9-methyl-dodecyl group, 10-methyl-undecyl group or 11-methyl-dodecyl group, is obtained as antibiotics having excellent antibacterial activities against bacteria and having a novel molecular structure. These tripropeptins each have an excellent antibacterial activity against various bacteria and drug-resistant strains thereof, such as methicillin-resistant strains and vancomycin-resistant strains.

The inventions relate to the field of medicine, veterinary and biotechnology and may be used for production of a drug for medical and veterinary purposes. A bacteriolytic complex, produced by the bacterium Lysobacter sp. XL1, has been proposed, which contains bacteriolytic enzymes (muramidase, muramoylalanineamidase, endopeptidase, a bacteriolytic enzyme with a molecular weight of about 22 kDa), protease, polysaccharide, and ballast components. The method of production of the bacteriolytic complex includes cultivation of the strain-producer on a nutrient medium containing glucose, peptone, yeast extract or yeast autolysate, phosphate salts of sodium and potassium, magnesium sulfate, potassium chloride, iron sulfate, and water.

The invention relates to a preparation and an application of a special mildew preventing agent for bamboo wood and bamboo products. The preparation process comprises the following steps of: using a slant culture medium to activate and culture a lysobacter enzymogenes C3 bacterial strain; transferring the bacterial strain to seed culture liquid; carrying out culturing for 48h at 24-30 DEG C and 230rpm; transferring the bacterial strain to fermentation culture liquid in 10% proportion; and culturing to obtain the mildew preventing agent. The mildew preventing agent can resist a heat treatment for 1h at 70 DEG C or can be stored for more than 180 days without a remarkable activity influence; after the mildew preventing agent treats the bamboo wood in three methods, namely soaking (for more than 4h), uniformly spraying or daubing, a mildew preventing effect is very remarkable; the bamboo wood keeps an original color andperformance. The invention provides a high-efficient biological mildew preventing agent and the application of mildew prevention of the bamboo wood; production cost is low; the conventional microbial fermentation equipment and a technology can be utilized for mass production; and source is very convenient. Mildew-preventing and preservative effects of the bamboo wood is very remarkable by different treatment methods, therefore, the mildew preventing agent has a wide application prospect in preventing mildew and corrosion of the bamboo products.

The invention belongs to the technical field of antibacterial drugs, and discloses an anti-wild lysobacter drug and an application thereof, and the anti-wild lysobacter drug is composed of 2 [mu]g / mLTPAD and 5 pM LED209. A low concentration of TPAD is capable of activating a QseC / B bi-component system (QseC is a membrane-bound sensor protein having histidine kinase activity and QseB is a cytoplasmic response modulator). In the lysobacter with the QseC or QseB gene knocked out, the TPAD can play a stronger antibacterial effect. The invention finds that efficient antibacterial activity can be generated when 2[mu] g / mL of TPAD and 5pM LED209 are used in combination.

The invention discloses a composite flora for preventing and controlling the outbreak of maize seed fusarium and application of the composite flora. The compound flora is prepared from acinetobacter baumannii RFAci-1, pseudomonas stutzeri RFPse-1, pseudoxanthomonas japonicum RFPth-1, burkholderia cepacia RFBur-1, stenotrophomonas maltophilia RFSte-1, bacillus amyloliquefaciens RFBac-1, lysobacter soilis RFLys-1 and enterobacter ludwigii RFEnt-1. The composite flora is stable in structure, has the characteristics of efficient biocontrol and growth promotion, can significantly reduce the morbidity of fusarium diseases caused by seed fusarium in main maize planting varieties in China after being inoculated into soil, and can also promote the growth of corn plants.

The invention relates to a microbial source bactericide for controlling cucumber powdery mildew. The microbial source bactericide consists of the following raw materials by weight: 1-30wt% of coniferous tree essential oil, 20-50wt% of fatty acid, 1-30wt% of an emulsifier, 0.1-10wt% of a polyvalent alcohol, 10-50wt% of water, 0.1-1wt% of a microbial metabolism product. The components are mixed to form the powdery mildew control agent. The fermented antibiotic Lysobacter's metabolite is organically combined with a protective agent and a dispersing agent to prepare the biocontrol preparation. The biocontrol preparation has the characteristics of rapid penetration, fast efficacy, and wide resistance, and all the raw materials are in line with the organic cultivation standards.

The invention belongs to the technical field of protease, particularly relates to keratinase, and further relates to application of the keratinase in hydrolysis of animal cutin raw materials. The keratinase LbKer is derived from lysobacter CGMCC No.16072, the tolerance temperature of the keratinase LbKer is higher than 55 DEG C, the keratinase LbKer has enzyme activity in the pH range of 5.0-9.0, the animal keratin raw material degradation rate of crude enzyme liquid fermented by the keratinase for 12 h reaches 60% or above, the keratinase LbKer has an amino acid sequence shown as SEQ ID NO.1 in a sequence table, and the keratinase with the amino acid sequence is a novel protein sequence reported for the first time at present, and has low similarity with the reported keratinase protein sequence. The keratin provided by the invention has the greatest advantages that the keratin has the characteristics of wide substrate spectrum, high wool degradation efficiency and the like.

The invention provides lysobacter solani and application thereof, and relates to the technical field of microorganisms. The lysobacter solani provided by the invention is lysobacter solani FTB-N1, and is preserved in the General Microbiological Center of China Committee for Culture Collection of Microorganisms on September 2, 2021, and the preservation number of the lysobacter solani is CGMCC No. 23348. The bacterium has the effects of fixing nitrogen and passivating heavy metals, and can convert free nitrogen in air into nitrogen which can be absorbed by crops, so that the nitrogen utilization of the crops is improved; the method can effectively passivate exchangeable cadmium in cadmium-polluted soil, reduce the effectiveness of the exchangeable cadmium, reduce the stress effect of cadmium on crop growth, prevent cadmium from being transferred into crop plants, reduce the cadmium content of crops, and improve food safety.

The invention belongs to the technical field of biological prevention and control, and discloses lysobacter enzymogenes and application thereof.The lysobacter enzymogenes are lysobacter enzymogenes BN3-15 strains, the strains are separated from rhizosphere soil of fresh corn in Dai autonomous zhou of Xishuangbanna in Yunnan province, and the strains are classified and named as lysobacter enzymogenes; the lysobacter enzymogenes BN3-15 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the lysobacter enzymogenes BN3-15 is CGMCC No. 21259. The lysobacter enzymogenes BN3-15 provided by the invention is safe and residue-free, has the effects of preventing diseases and promoting growth, can be prepared into an aqueous solution, wettable powder or a seed coating agent, and is used for preventing and treating diseases such as northern corn leaf blight, pepper anthracnose, Chinese cabbage clubroot, panax notoginseng root rot and the like in fields. The biological agent provided by the invention is relatively good in comprehensive character, remarkable in fungal disease control effect, beneficial to reducing the use amount and residual quantity of chemical agents in agricultural products, relatively safe to use and easy for industrial production.

The invention discloses a quick detection kit for antibiotic lysobacter loop-mediated isothermal gene amplification, and a detection method. The kit carries out large-scale genome analysis by virtue of a biological information platform; according to the phenazine synthesis gene phzF of antibiotic lysobacter, four specific primers are designed; a LAMP (Loop-Mediated Isothermal Amplification) technology is combined to establish a quick, sensitive and accurate detection method by aiming at the antibiotic lysobacter, and a quick detection kit used for the method is constructed. When the quick detection kit and the detection method are used, observation and identification are carried out through naked eyes after reaction is carried out, no other analysis steps including electrophoresis and the like are required, and the quick detection kit has the advantages of short detection time, high specificity, low instrument equipment requirement, convenience in operation and the like and can be used for the quick detection of the antibiotic lysobacter.

The invention discloses colloidal lysobacter OH17 and application thereof in plant growth. The colloidal lysobacter OH17 contains cystathionine gamma-synthase genes for synthesis of 1-aminocyclopropane-1-carboxylic acid (ACC) and ACC deaminase genes. The ACC deaminase genes can decompose the ACC into alpha-ketobutyric acid and ammonia, the ACC is the synthesis precondition of ethylene, generationof the ethylene is effectively reduced through ACC deaminase, and thus the adaptive capacity of plants under adversity stress is improved. Compared with the prior art, the generation amount of the ethylene is effectively decreased through the genes for ACC synthesis in the colloidal lysobacter OH17, the survival rate of the plants is increased, the pest-resistant capability is enhanced, and the plant growth condition is improved.

The invention discloses a method for increasing the yield of an antifungal active substance HSAF and application. According to the invention, an RNA chaperone molecular protein Hfq on a lysobacter enzymogenes OH11 strain genome is knocked out by using a homologous recombination method, so that a strain delta Hfq is obtained. The strain delays the autolysis phenomenon, so that the effective fermentation time is prolonged, the yield of the active metabolite HSAF of the strain is increased, and a foundation is laid for industrial development of the HSAF.

The invention discloses a self-dissolving solution for a novel lysobacter enzymogenes strain LE16. The self-dissolving solution is prepared from the following raw material components in parts by weight: 10 to 20 parts of peptone, 5 to 15 parts of beef extract, 5 to 15 parts of NaCl, 0.5 to 3 parts of tryptophan betaine, 0.5 to 3 parts of asparagine, 0.05 to 0.5 part of multivitamins, 1 to 10 parts of (NH4)2SO4, 5 to 15 parts of glucose, 1 to 5 parts of KH2PO4 and 0.5 to 3 parts of MgSO4. The prepared self-dissolving solution of the LE16 does not contain thalli, does not need filtration sterilization, has a good and stable effect of preventing and treating powdery mildew and gray mold of plants, is long in storage time, is slightly influenced by conditions such as environments, and is simple in application method, high in practicability and easy to popularize, a technical system of a preparation process is simple and universal in equipment, low in resource consumption and low in industrial production requirements, and a general microbial preparation preparing factory can meet the production requirements.