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Lysobacter and application thereof

A technology of lysobacteria and bacterial strains, which is applied in the field of microorganisms, can solve the problems of no public reports of lysobacteria, and achieve the effect of non-toxic natural medicine, stable and long-lasting effect, and mellow smoke effect

Inactive Publication Date: 2018-06-29
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention isolates a lysobacterium for the first time, which has been identified as a new species of microorganism. At present, there is no public report about the lysobacterium and related applications

Method used

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  • Lysobacter and application thereof
  • Lysobacter and application thereof
  • Lysobacter and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] (1)—Isolation of Lysobacterium C8-1

[0022] Tobacco rhizosphere soil samples were collected in tobacco planting areas in Kunming City, Yunnan Province, sealed in plastic bags, and stored at 4°C for later use. Accurately weigh 5g of soil sample, add 45mL of sterile water, shake at 30°C and 180rpm for 1h. Dilute to 10 with sterile water -1 、10 -2 、10 -3 、10 -4 、10 -5 Concentration, respectively take 0.2mL spread on LB plate culture medium, set 3 replicates for each. After culturing at 30°C for one week, different single colonies were picked from the plates of each soil sample, purified on the corresponding solid medium, added glycerol at 20% of the bacterial volume, and stored in a -80°C refrigerator for later use.

[0023] The isolated microorganisms were respectively inoculated in ISP 2 liquid medium, shaken and cultivated at 30°C and 160 rpm on a shaker, and the bacteria were cultured overnight until the concentration of the bacterial solution was OD=2.0, which ...

Embodiment 2

[0036] ——Application of the lysobacterium C8-1 screened above in the fermentative production of tobacco spices, the specific steps are as follows:

[0037] (1) Slant culture: medium: glucose 0.5g; yeast extract 0.5g; peptone 0.5g; acid hydrolyzed casein 0.5g; soluble starch 0.5g; sodium pyruvate 0.3g; g; agar 15g; distilled water 1000ml, pH7.2; culture temperature is 30℃, culture time is 7d.

[0038] (2) Seed culture: pick part of the mycelium from the slope and put it into the seed liquid, and shake the flask for 36 hours. Seed medium: dextrin 120g; soybean powder 40g; yeast extract 2g; tryptophan 0.5g; β-alanine 5g; magnesium sulfate 0.5g; ammonium phosphate 0.2g; ℃.

[0039] (3) Fermentation culture: Inoculate 10% of the inoculum into the fermentation medium, and culture in shake flasks for 6 days. Medium: 10g of soybean powder; 10g of glucose; 3g of peptone; 2.5g of sodium chloride; 2g of calcium carbonate;

[0040] The fermented liquid is reflux extracted, the residue...

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Abstract

The invention discloses lysobacter sp and application thereof. The strain is named as lysobacter sp C8-1, is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 28, 2017, and has a preservation number of CGMCC No.1.16271. Microbiological characteristic analysis and 16S rRNA gene sequence analysis show that the strain is a new type of bacteria. The strain can produce benzaldehyde and volatile compounds such as geosmin, elemene, elemol and alpha-eudesmol, and can be used for preparing tobacco flavor after subjected to fermentation. The obtained flavor has very special aroma, thus being capable of enhancing the style of cigarettes, enriching cigarette fragrance, and enabling the aroma to be mellow and fuller; furthermore, the strain has antibacterial andfungal activity, and is stable and lasting in effect. In addition, the strain has great potential in development of novel, highly effective, non-toxic natural medicines.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a new lysobacterium. Meanwhile, the present invention also relates to the application of the lysobacterium. Background technique [0002] There are many types of microorganisms. For a long time, people have used pure culture technology to investigate, research, develop and utilize microorganisms in the environment. With the development of science and technology, the importance of microbial resources in scientific research and biological industry has become increasingly prominent. Many countries in the world are collecting and preserving various microbial resources, and their continuous enrichment will lay a solid material foundation for the development of my country's biotechnology. [0003] Flavoring is a key link in the cigarette process, and it is one of the effective means to effectively improve the smoking quality of cigarettes and form a unique style of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B3/12A24B15/26A61K35/74A61P31/04C12R1/01
CPCA24B3/12A24B15/26A61K2035/11C12N1/205C12R2001/01
Inventor 周国福段焰青杨洪明刘秀明廖头根李文均雷声
Owner CHINA TOBACCO YUNNAN IND
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