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Biological seed coating agent and preparation method thereof

A biological and additive technology, applied in botany equipment and methods, biocides, chemicals for biological control, etc., can solve the problems of easy resistance of bacteria, environmental pollution of chemical agents, high pesticide residues, etc. Achieving the effects of less drug resistance, environmental friendliness, and simple preparation method

Inactive Publication Date: 2019-10-22
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main use of chemical agents as active ingredients in seed coatings is used to prevent and control diseases. Chemical agents have problems such as environmental pollution, high pesticide residues, and easy resistance of bacteria, which is contrary to the current development of green agriculture. Therefore, it is necessary to choose suitable biological agents. It is imminent to prevent and control diseases in agricultural production

Method used

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  • Biological seed coating agent and preparation method thereof
  • Biological seed coating agent and preparation method thereof
  • Biological seed coating agent and preparation method thereof

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preparation example Construction

[0029] The present invention also provides a kind of preparation method of biological seed coating agent comprising the following steps:

[0030] (a) Inoculate the antibiotic lysobacterium in the KB liquid culture medium, carry out shake flask fermentation culture at 28°C and 160r / min for 48h to prepare the fermentation liquid, the initial antibiotic lysobacterium content ≥ 1×10 9 cfu / mL;

[0031] (b) mix polyvinyl alcohol with chitosan mixture, OP-10, sodium lignosulfonate, xanthan gum, glycerol, eosin, stir evenly, then put into grinder and grind, and the grinding time is 4 to 8 hours, grind until the average particle size of the material is less than 5 μm, and prepare it as an additive;

[0032] (C) Mix the antibiotic lysobacterium fermentation liquid obtained in step (a) with the auxiliary agent obtained in step (b) at a volume ratio of 1:99 to prepare a biological seed coating agent.

[0033] Further, the content of each component in the auxiliary agent is 3-5% of polyvin...

Embodiment 1

[0039] Example 1 Bio-type seed coating agent for growth promotion and clubroot control effect on cruciferous vegetables

[0040] Taking cabbage as a typical representative crop of cruciferous vegetables, the growth promotion and clubroot control effect of antibiotic Lysobacterium biotype seed coating agents on cruciferous vegetable crops were tested.

[0041] Experimental location and experimental design: The experimental location is the cabbage clubroot experimental field in Lufeng County, Yunnan Province. The cabbage variety "Luchunbai No. 1" was selected for coating treatment of cabbage seeds. Four treatments were set up in the experiment: antibiotic lysobacterium biological seed coating agent, biocontrol bacteria Bacillus subtilis biological type seed coating agent, chemical pesticide cyanazazole chemical type seed coating agent coated seeds and uncoated seeds CK, each treatment Repeat 3 times, a total of 12 plots, 5 rows of cabbage were planted in each plot, 42 plants in ...

Embodiment 2

[0061] Example 2 Biotype Seed Coating Agent Effect on Maize Growth Promotion and Maize Leaf Spot

[0062] A biological seed coating agent with the effect of promoting growth and disease prevention, each component of the biological seed coating agent is 4% film forming agent (mass volume ratio) and 3% wetting agent (mass volume ratio) by volume and weight ratio per 100mL , dispersant (mass volume ratio) 0.5%, thickener (mass volume ratio) 0.05%, protective agent (mass volume ratio) 6%, warning color (mass volume ratio) 1%; initial antibiotic lysobacterium content ≥ 1 × 10 9 cfu / mL of fermentation broth. Mix the adjuvant with the antibiotic lysobacterium fermentation broth at a ratio of 1:99. The biocontrol fungus "Trichoderma" was used as the active ingredient as the positive control, the chemical pesticide tebuconazole was used as the active ingredient as the negative control, and uncoated corn seeds were used as the blank control CK.

[0063] The preparation of the above-me...

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Abstract

The invention belongs to the technical field of seed coating agents, and relates to a biological seed coating agent and a preparation method thereof. The preservation information of a beneficial bacterial strain is CGMCC No.2403 2008.03.17. The biological seed coating agent comprises beneficial bacteria and an auxiliary agent, wherein the beneficial bacteria and the auxiliary agent are mixed in proportion; the beneficial bacteria are antibiotic lysobacter; the auxiliary agent comprises a film forming agent, a wetting agent, a dispersing agent, a thickening agent, a protective agent and warningcolor; the film forming agent is formed by mixing polyvinyl alcohol and chitosan; the wetting agent is OP-10; the dispersing agent is sodium lignosulfonate; the thickening agent is xanthan gum; the protective agent is glycerol; and the warning color is eosin. The preparation method comprises the following steps: uniformly mixing antibiotic lysobacter and the auxiliary agent according to a proportion to prepare the biological seed coating agent. The biological seed coating agent has no influence on plant growth safety, belongs to an environment-friendly preparation, and is suitable for the requirement of sustainable green agricultural development.

Description

technical field [0001] The invention belongs to the technical field of seed coating agents, in particular to a biological seed coating agent and a preparation method thereof. Background technique [0002] Seed coating agent is a new seed processing technology developed on the basis of traditional seed soaking technology. It uses various auxiliaries to coat the active ingredients on the outside of the seeds to achieve the purpose of disease resistance, growth promotion and production increase of crops. As a new technology for seed treatment, seed coating agent meets the requirements of modern agricultural development. At present, the main use of chemical agents as active ingredients in seed coatings is used to prevent and control diseases. Chemical agents have problems such as environmental pollution, high pesticide residues, and easy resistance of bacteria, which is contrary to the current development of green agriculture. Therefore, it is necessary to choose suitable biolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/30A01N25/22A01N63/02A01N63/00A01P21/00A01P3/00A01P1/00
CPCA01N25/30A01N25/22A01N63/00A01N63/10
Inventor 魏兰芳姬广海杨俊张荣琴代真林
Owner YUNNAN AGRICULTURAL UNIVERSITY
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