41 results about "Bacteriolytic enzyme" patented technology

The invention relates to an echinacea extract and a preparation method of the echinacea extract. The echinacea extract provided by the invention is obtained through carrying out twice extraction on echinacea medicinal materials by alkaline ethanol solution and lysozyme; and in the product, the polysaccharide content is greater than or equal to 21.50 percent, the polyphenol content is greater than or equal to 8.10 percent, and the chicoric acid content is greater than equal to 5.00 percent. Because the polyphenol content and the chicoric acid content of the echinacea extract are obviously increased, the echinacea extract is prepared into ordinary preparations including powder or oral liquid and the like, and the curative effect is greatly improved. In a compound preparation processed by the echinacea extract and bacteriolysant according to a consumption ratio being 3:1, active ingredients of echinacea and lysozyme totally account for 20 percent, wherein the consumption ratio of the echinacea extract to the lysozyme is (4-1):1, auxiliary materials of cane sugar account for 64 percent, and dextrin accounts for 16 percent. The compound preparation has the functions of improving the immunity of live stock and realizing the sterilization and antivirus functions, and can be used as medicine for preventing and treating animal diseases, particularly chicken diseases caused by escherichia coli infection.

It relates to a bactericide, comprising lysostaphin 0.0001í½0.1wtúÑú¼lysozyme 10í½50wtúÑú¼stabilizer 1í½15wtúÑú¼surfactant 0.1í½0.5wtúÑú¼water. It also relates to an antiseptic wet towel, its preparation method and applications in treatment and prevention of mammary gland infection of milch cow. The bactericide can be carried by a carrier, then an antiseptic wet towel is obtained. The antiseptic wet towel could kill all bacteria causing mammary gland infection of milch cow. The killing rate for bacillus coli, staphylococcus aureus and the like, is above 99%.

The invention relates to embroidery artware antibiosis and mildew-proof care solution and a preparation method of the embroidery artware antibiosis and mildew-proof care solution. The embroidery artware antibiosis and mildew-proof care solution and the preparation method of the embroidery artware antibiosis and mildew-proof care solution are used for mildew-proof processing for embroidery threads and embroidery pieces in an embroidery artware manufacturing process and before the embroidery threads and the embroidery pieces are stored. The embroidery artware antibiosis and mildew-proof care solution and the preparation method of the embroidery artware antibiosis and mildew-proof care solution overcome shortcomings of the prior art, and have the advantages of being colorless, tasteless, free of corrosivity for real silk, non-irritant for skin, significant in antibiosis performance, free of decoloration phenomenon after being used, capable of preventing generation of germs and mildew, easy and convenient to use, simple in process, and the like. The technical scheme includes that the solution comprises the following ingredients by weight: 2.0-4.0% of carboxymethyl chitosan, 2.0-3.0% of algal polysaccharides, 6.0-8.0% of marine organism muramidase and 100% of purified water. The preparation method includes culturing the marine organism muramidase for backup use according to a culturing method disclosed in a Chinese patent CN200410097081, and then performing an emulsifying mixing process which includes adding the purified water required in the ingredient into an emulsifying tank and heating the purified water to be at 40 DEG C, adding the carboxymethyl chitosan and the algal polysaccharides into the emulsifying tank according to the proportion in the ingredient to be mixed for 45 minutes, adding the marine organism muramidase into the tank to be mixed for 15 minutes when the temperature of the solution declines to be at 30 DEG C, and canning and packaging the solution after the solution is cooled.

The invention relates to the technical field of aquatic product processing, in particular to a fresh-keeping method for tuna. The specific method is as follows: remove the viscera and gills of the fresh fish after catching, and clean the surface and inside of the fish body with a cleaning solution; 30min; finally put the soaked fish into binary ice for storage, and the binary ice is prepared by dissolving the following components in weight percentage in water: foaming agent 0.5-2%, organic acid 0.5-3%, Sodium chloride 6-15%, surfactant 0.1-0.3%, vitamin E 1-3%. The method of the invention can effectively inhibit the multiplication of various bacteria in the fish meat for a long time, the meat quality and appearance of the tuna after preservation are less changed, and the residue of harmful chemical substances is extremely low, so that the tuna is kept fresh for a long time on the basis of ensuring the quality of the fish meat.

A health-care food for protecting liver, nourishing stomach, and improving immunity is prepared from the artificially cultured fly maggot through dual physical inducing and proportionally adding the extract of fly maggot shell. Its advantage is rich nutrients (insection protein, amino acids, etc) and medicinal components (antibacterial peptide, chitosan, etc).

The invention discloses enzyme supplementation gum which is composed of 0.1%-0.3% of muramidase, 18%-22% of aluminium hydroxide, 2%-3% of vitamin C, 0.6%-1% of citric acid, 2%-3% of glycerinum, 20%-30% of gum base, 40%-45% of sorbitol, 4%-6% of mannitol, 0.5%-1% of essence and 6%-10% of water. According to the enzyme supplementation gum, the biological muramidase and the citric acid are added, the pH value of the gum can keep the activity of the muramidase to the maximum degree, and bacteria resistance and bacteriostasis, dental calculus prevention, caries prevention, ozostomia relieving, dental plaque inhibition and remarkable improvement of gingival bleeding are conducted on the aspect of biology; the aluminium hydroxide and the vitamin C can assist the muramidase in removing dental plaque and stain, the effect is more remarkable, gingival bleeding is inhibited, and the gum can taste sour and sweet and delicious; the teeth can be healthy and white and breath can be fresh through long-term use of the enzyme supplementation gum.

The invention belongs to the technical field of nano material preparation, and discloses a method of preparing antimicrobial nanofiber complex film of polyvinyl alcohol / muramidase / ethylene diamine tetraacetic acid (EDTA) from electrostatic spinning and the application of the antimicrobial nanofiber complex film of the polyvinyl alcohol / muramidase / EDTA. The preparation comprises the following steps: solving the polyvinyl alcohol in distilled water, carrying out magnetic stirring and obtaining polyvinyl alcohol solution; cooling the obtained polyvinyl alcohol solution to room temperature, and adding the muramidase and the EDTA to the polyvinyl alcohol solution; carrying out magnetic stirring to obtain spinning solution; and sucking the spinning solution into an injection syringe, controlling the flow speed of the injection syringe, spinning, drying in vacuum, and obtaining the antimicrobial nanofiber complex film with the biological activity. The antimicrobial nanofiber complex film with the biological activity has obvious bacteriostasis to escherichia coli and staphylococcus aureus. The staphylococcus aureus has good toughness and low toxicity features, and has wide application prospect in the field of food packaging materials and biological materials.

The invention belongs to the field of biochemistry, and discloses an antibacterial protein LYS-ABP and an antibacterial protein MBP-LYS-ABP having an LYS-ABP protein N terminal added with MBP, and preparation methods thereof, wherein the antibacterial protein LYS-ABP and the antibacterial protein MBP-LYS-ABP have no stimulation to human bodies and have good antibacterial effect. The antibacterial protein LYS-ABP and the antibacterial protein MBP-LYS-ABP have no stimulation to the human bodies and have good antibacterial effect, the antibacterial effect is better than single muramidases and antibacterial peptide proteins, and the biological activity stability is good. The antibacterial protein MBP-LYS-ABP is obtained by in-vitro recombinant expression, and is easy to prepare and relatively low in cost; and the antibacterial protein MBP-LYS-ABP has high soluble expression yield and is suitable for large-scale preparation. The antibacterial protein LYS-ABP is obtained by enzyme digestion of a recombinant expression-prepared MBP-LYS-ABP fusion protein with thrombin, and is easy to prepare and relatively low in cost.

The invention provides a fusion protein. The fusion protein is formed by connecting an anti-iysozyme antibody or a variable region fragment of the anti-iysozyme antibody with a horseradish peroxidase reporter gene protein in series, the invention further provides a preparation method and an application of the fusion protein. The fusion protein is used for immunoassay which takes an immunolabelling technique as a basis, so that good biological activities of enzymes and the antibody are kept, and the detection efficiency is higher.

The invention is applicable to the technical field of an oral cavity health care product, and provides mouth wash and a preparation method thereof. The mouth wash is prepared from the following ingredients in percentage by weight: 0.45 to 0.6 percent of nonionic surfactants, 0.5 to 1.5 percent of enzyme preparations, 0.1 to 0.15 percent of preservatives, 0.1 to 0.2 percent of pH conditioning agents and the balance of deionized water, wherein the enzyme preparations are mixtures of at least three kinds of materials from glucanase, glucose oxidase, polyphenol oxidase, muramidase, coenzyme Q10, amylase, trypsin, catalase, bromelin or protease. The mouth wash is weakly acidic; stimulating ingredients such as ethyl alcohol are not contained; the mouth wash is mild and inirritative on the oral cavity; food-grade raw materials are used; safety and nontoxicity are realized; meanwhile, various kinds of enzymes are compounded; various enzymes can maintain higher enzyme activity in the recipe; teeth stain can be effectively removed; the growth of dental plaque can be inhibited; the effects of obviously relieving gingival bleeding, reliving gingivitis, improving oral cavity natural defense capability and creating good oral environment can be achieved.

The invention relates to a compound biological pesticide for preventing and treating a banana wilt-panama disease. According to the occurrence characteristic of the banana wilt-panama disease, two biological inductive agents are selected by agent screening, and the two biological inductive agents and biological lysozyme are subjected to combination compound, so that a formula has a function of inducing a banana to generate disease resistance and has a bactericidal effect, and a purpose of preventing and treating the banana wilt-panama disease is achieved; the compound biological pesticide is nontoxic, harmless and pollution-free; a use method is simple; the compound biological pesticide is injected at a well-known room temperature and time and root irrigation is performed for three to five times; the cost is lower; and implementation is easy.

Method for extraction and purification of plasmid DNA from prokaryotes and its preparation process, wherein the process comprises the steps of prokaryote bacterium liquid concentration, splitting thalline, and plasmid DNA purification.

The invention relates to a complex enzyme preparation for a leaching tobacco material and an enzyme leaching method, and belongs to the technical field of regenerated tobacco processing. The active component of the complex enzyme preparation comprises 0.1-1000U of lysozyme, 1-1000 U of cellulase, 0.1-1000U of alpha-amylase, 0.1-1000 U of proteolytic enzyme and 0.1-1000U of pectinase, wherein the amount is the adding amount in per gram of tobacco material. The temperature in leaching is controlled at 30-60 DEG C, and the liquor ratio is 1:5-10; the enzyme preparation is added to be stirred for 1-4 hours; and after solid-liquid separation, solid phase is taken and water is further added according to original solid weight for circularly extraction for 1-2 times. According to the invention, mesophyll cell and cabo fiber structures, composition, physical properties and the like of tobacco are adequately considered, combined wall breaking effects of lysozyme, pectinase and cellulase are introduced, and enzyme catalytic action of proteolytic enzyme, alpha-amylase and cellulase for degrading biomacromolecule is considered, so that the compact structure of tobacco and straw is dredged, leach of inclusions of materials is increased, the dissolution rate is enhanced and the quality of the extracting liquid is improved.

The biological dressing includes chitosan, acetic acid and biological enzyme, preferably FE complex enzyme. It is prepared through dissolving chitosan in distilled water and acetic acid, mixing with biological enzyme via stirring and filtering. It is used in wound treatment, and during treatment, particle dressing is self-degraded into oligosccharide to kill germ fast and to become wound healing agent while the undergraded part forms film natural barrier to inhibit germ's invasion. The present invention has no toxic harm, no allergy and no irritation to body.

The invention belongs to the technical field of surfactants, and discloses a preparation method and application of a rhamnolipid composite surfactant. The preparation method comprises the following steps: adding lysozyme into rhamnolipid fermentation liquor which is totally fermented, conducting stirring and reacting under the conditions that a temperature is 45-50 DEG C and a pH value is 5-7, cooling the obtained fermentation liquor to room temperature, then adding APG, carrying out stirring and washing, then adding polyaluminum chloride, carrying out flocculent precipitation and centrifugation, taking a supernatant, and concentrating and drying the supernatant to obtain the rhamnolipid-APG composite surfactant. According to the preparation method disclosed by the invention, lysozyme is used for treating the fermentation liquor, and APG is used for washing, so the yield of rhamnolipid can be remarkably increased; and polymeric aluminum chloride is adopted to carry out flocculent precipitation on the fermentation liquor having been subjected to bacteriolysis treatment, so the purity of the product is improved. The obtained product has good surface activity, and has the advantages of being natural, environment-friendly, non-irritant and biodegradable when being applied to daily chemical washing products.

The invention relates to the technical fields of molecular biology and genetic engineering, and aims at providing a bemisia tabaci C-type lysozyme Btlys-c gene as well as a preparation and an application of an encoded protein. A nucleotide sequence of the bemisia tabaci C-type lysozyme (Btlys-c) gene is as shown in SEQ ID NO.1. The the bemisia tabaci C-type lysozyme Btlys-c gene provided by the invention and the encoded nucleotide sequence thereof can be applied to expression of new protein and encoded sequence in escherichia coli; the result of a bacteriostasis experiment after purification of a recombinant protein with antibacterial activity proves that the recombinant protein for prokaryotic expression of the recombinant bemisia tabaci C-type lysozyme (Btlys-c) gene has the ability of resisting gram-positive bacterium; and furthermore, the bemisia tabaci C-type lysozyme Btlys-c gene is produced through an existing genetic engineering method by using the method disclosed by the invention, and has potential application value in the aspects such as development of antibacterial drugs, food preservatives and feed additives.

The invention relates to a preparation method of a protein magnetic imprinting nano sphere, in particular to a preparation method of a protein magnetic imprinting nano sphere which takes one of hemoglobin, muramidase or serum protein as template molecules; the preparation method includes the following steps: synthesizing magnetic nano particles which are Fe3O4 nano particles covered with silicon dioxide on the surfaces; carrying out silanization to the amino-groups on the surface of the magnetic sphere; using glutaric dialdehyde to connect protein; using silylation agent to fix the space structure of template protein; alkaline eluting the protein on the surface of the magnetic sphere to form imprinting sites. The hemoglobin, muramidase or serum protein magnetic nano imprinting polymer sphere prepared by the invention combines the precise and specific identification property of molecular imprinting and the quick separation property of the magnetic nano sphere under the action of an external magnetic field, integrates the advantages of molecular imprinting and the magnetic nano sphere, avoids complex centrifugation operation, plays vital roles in the separation of cells, protein andnucleic acid, the detection of biomolecules, tumor diagnosis and medicine targeting therapy and has promising application prospect in the field of isolation analysis.

The present invention discloses a bacteriostatic protein and an encoding gene and an application thereof and a strain method. Chicken lysozyme is used as a starting template, an amino acid sequence ofscorpion-derived antimicrobial peptide IsCT is superposed to an N terminal of the chicken lysozyme protein through rational design, and meanwhile, an antioxidant peptide joint and an amino acid jointare directionally introduced to obtain a mutant LyIc; then according to a codon preference of pichia pastoris, a whole-gene synthesis technology is adopted to obtain a gene lysIs corresponding to themutant LyIc and a nucleotide sequence of the lysIs is shown as SEQ ID NO.1; then the lysozyme mutant gene lysIs is linked into an expression vector pPICZ alpha A, the vector is transferred into pichia pastoris X33, the recombinant microorganisms are subjected to fermentation culture and the lysozyme mutant LyIc is separated and purified; and finally, bacteriostatic effects of the lysozyme mutantLyIc and original lysozyme on common pathogenic bacteria are compared and analyzed. The lysozyme mutant LyIc effectively improves the bacteriostatic effect on common pathogenic bacteria (escherichia coli, salmonella and staphylococcus aureus) and lays foundation for wide application of the chicken lysozyme.

The invention provides a biological enzyme composite disinfectant as well as a preparation method and application thereof. The method comprises the following steps: dissolving folium eriobotryae, bamboo leaves, dandelion and houttuynia cordata in deionized water, heating the liquid to 100 DEG C, boiling the liquid for 30-60 minutes, and cooling the liquid; sequentially adding lysozyme and the plant extracting solution into the cooled water, and magnetically stirring the solution for 1-2 hours until the solution is uniformly mixed; and adding spices as required, uniformly stirring and sub-packaging the products. The enzyme composite disinfectant has good antibacterial and bactericidal effects, and the antibacterial and bactericidal rate reaches 99.9%; the preparation process is relatively simple and easy to operate.

The invention relates to application of a snow tea extract to preparation of a medicine for preventing and treating zymloid protein diseases. The influence on zymloid protein fiber formation by the snow tea extract is mainly explored, a new effective treatment medicine for preventing and treating zymloid deposition diseases is expected to find, and support is provided for medicinal research on thesnow tea. Typical zymloid protein chicken lysozyme is mainly subjected to in-vitro culture to form fiber and secreted protein of a recombinant pichia pastoris strain of chicken cystatin forms fiber.The result indicates that the snow tea extract achieves the effect of inhibiting the zymloid protein from forming the fiber; furthermore, in the water extract group, the effect is more significant andthe IC50 value is about 0.018 mg / mL. The bacterial level protein expression experiment indicates that the snow tea extract can inhibit a chicken cystatin zymloid mutant I66Q from forming aggregate extracellularly in a concentration dependency way; and when the extract concentration reaches to 1.5 mg / mL, a certain inhibition effect can be generated on oligomerization and aggregation of the secreted protein.

The inventions relate to the field of medicine, veterinary and biotechnology and may be used for production of a drug for medical and veterinary purposes. A bacteriolytic complex, produced by the bacterium Lysobacter sp. XL1, has been proposed, which contains bacteriolytic enzymes (muramidase, muramoylalanineamidase, endopeptidase, a bacteriolytic enzyme with a molecular weight of about 22 kDa), protease, polysaccharide, and ballast components. The method of production of the bacteriolytic complex includes cultivation of the strain-producer on a nutrient medium containing glucose, peptone, yeast extract or yeast autolysate, phosphate salts of sodium and potassium, magnesium sulfate, potassium chloride, iron sulfate, and water.

The invention relates to an echinacea extract and a preparation method of the echinacea extract. The echinacea extract provided by the invention is obtained through carrying out twice extraction on echinacea medicinal materials by alkaline ethanol solution and lysozyme; and in the product, the polysaccharide content is greater than or equal to 21.50 percent, the polyphenol content is greater than or equal to 8.10 percent, and the chicoric acid content is greater than equal to 5.00 percent. Because the polyphenol content and the chicoric acid content of the echinacea extract are obviously increased, the echinacea extract is prepared into ordinary preparations including powder or oral liquid and the like, and the curative effect is greatly improved. In a compound preparation processed by the echinacea extract and bacteriolysant according to a consumption ratio being 3:1, active ingredients of echinacea and lysozyme totally account for 20 percent, wherein the consumption ratio of the echinacea extract to the lysozyme is (4-1):1, auxiliary materials of cane sugar account for 64 percent, and dextrin accounts for 16 percent. The compound preparation has the functions of improving the immunity of live stock and realizing the sterilization and antivirus functions, and can be used as medicine for preventing and treating animal diseases, particularly chicken diseases caused by escherichia coli infection.

The invention discloses a codon-optimized danio rerio g-type lysozyme-1 gene and a recombinant expression protein thereof. The danio rerio has three lysozyme genes in total, namely c type lysozyme, g1 type lysozyme and g2 type lysozyme. The danio rerio g-type lysozyme-1 gene is obtained through optimization and is obtained by adopting a whole-gene synthesis mode after codon optimization, the nucleotide sequence of the danio rerio g-type lysozyme-1 gene is shown as SEQ ID NO.2, and the coded amino acid sequence of the danio rerio g-type lysozyme-1 gene is shown as SEQ ID NO.3. The gene is connected with a carrier pET-28a (+) to obtain a recombinant plasmid 28a-Lys-g1, the recombinant plasmid 28a-Lys-g1 is further transformed into escherichia coli BL21 (DE3) competent cells, and the danio rerio g-type lysozyme-1 recombinant protein with high purity and high bacteriolytic activity is obtained through induced expression, purification, SDS-PAGE verification and renaturation.

The invention provides a detection method of hereditary stability of gene vector for encoding muramidase-released protein and application, and belongs to the field of biological engineering. The detection method provided by the invention is characterized in that a host bacteria bacterial fluid containing gene vector plasmids for encoding muramidase-released protein is subjected to recovery culture, and is subjected to continuous multi-generation subculture as well as is sampled and detected at a certain generation interval, and the hereditary stability of the artificially preserved host bacteria containing the gene vector plasmids encoding muramidase-released protein is detected, thereby providing reliable support and guarantee for researching the gene vector for encoding muramidase-released protein. The detection method can be applied to the culture of the gene vector for encoding muramidase-released protein so as to provide reference for the research and the culture of the gene vector for encoding muramidase-released protein, and has important application values.

The invention relates to a processing method of lavender essential oil. Fresh lavender is enzymatically hydrolyzed with lysozyme, then inactivated, extracted with liquid carbon dioxide after inactivating the enzyme, and separated to obtain lavender essential oil. The invention has low extraction temperature and low pressure, which can effectively prevent damage to the essential oil by higher temperature and pressure, and improve the extraction rate; the invention adds a retaining agent, which can obviously prolong the service life of the essential oil.

The embodiment of the invention discloses a biological deodorant for pets and a preparation method thereof. The biological deodorant for pets comprises the following components: a plant extracting solution and a biological active enzyme, wherein the mass ratio of the plant extracting solution to the biological active enzyme is (2-10): (1-2); the plant extracting solution comprises a zanthoxylum bungeanum fruit extracting solution, a radix sophorae flavescentis extracting solution, an aloe barbadensis leaf extracting solution, a citrus peel extracting solution, a pseudolarix amabilis root and bark extracting solution and a persimmon fruit extracting solution; the bioactive enzyme comprises lipase, desulfhydrylase, oxidoreductase and lysozyme. According to the embodiment of the invention, the problems of low deodorization efficiency and incomplete deodorization of the existing deodorization product can be effectively solved, meanwhile, the biological deodorant for pets provided by the invention takes the plant extract liquid as a main component, and the plant extract liquid reacts with odor gas molecules to fundamentally remove odor and inhibit the reproduction and diffusion of bacteria after pets urinate and defecate, so that the biological deodorant for pets has a good deodorization effect. The pet food is safe, environment-friendly, free of secondary pollution and capable of meeting the requirement that the living environment of pets and owners thereof is odorless.

The invention discloses a method for constructing high-internal-phase emulsion gel based on a dihydromyricetin / egg lysozyme compound emulsifier, and belongs to the technical field of food. The method comprises the following steps: mixing dihydromyricetin and egg lysozyme and directly adding a mixture into water to serve as a water phase; taking edible oil as an oil phase; mixing the oil phase and the water phase, shearing at a high speed, and standing at room temperature to obtain the high-internal-phase emulsion gel. The prepared high-internal-phase emulsion gel is high in safety, good in stability and rich in natural antioxidant dihydromyricetin and edible bacteriostatic agent egg lysozyme, and can be used as a solid fat substitute and a functional component delivery body to be applied to the field of food.