Preparation method and application of rhamnolipid composite surfactant
A technology for compounding surfactants and rhamnolipids, which is applied to surface active detergent compositions, preparations for skin care, chemical instruments and methods, etc., can solve the problems of low yield and high preparation cost, and improve yield , Rich and delicate foam, strong compatibility effect
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Embodiment 1
[0032] A kind of preparation method of rhamnolipid complex surfactant of the present embodiment comprises following preparation steps:
[0033] (1) add lysozyme in the rhamnolipid fermented liquid, the add-on of regulating lysozyme is respectively 0.01%, 0.02%, 0.04%, 0.08% and 0.10% of the rhamnolipid fermented liquid quality, at a temperature of 45 ~50°C, stirring and reacting for 2 hours under the condition that phosphoric acid adjusts the pH to 5.5-6.5. And the fermentation broth without adding lysozyme was used as blank control.
[0034] (2) Cool the fermented liquid after the lysing treatment in step (1) to room temperature, then add APG to stir and wash, and the amount of APG added is 3.5% of the mass of the fermented liquid after the lysing process.
[0035] (3) adding polyaluminium chloride to the fermented liquid after step (2) washing carries out flocculation precipitation, and the flocculation precipitation maintenance system pH is about 6, and the add-on of polya...
Embodiment 2
[0041] A kind of preparation method of rhamnolipid complex surfactant of the present embodiment comprises following preparation steps:
[0042] (1) Add lysozyme to the rhamnolipid fermentation liquid, the addition of lysozyme is 0.04% of the quality of the rhamnolipid fermentation liquid, under the condition that the temperature is 45~50° C. and the pH adjusted by phosphoric acid is 5.5~6.5 The reaction was stirred for 2h.
[0043] (2) cooling the fermented liquid after step (1) lysing treatment to room temperature, then adding APG to stir and wash, adjusting the addition of APG to be respectively 0.5%, 1%, 2%, 3.5% of the fermented liquid mass after lysing treatment , 5% and 7.5%. And the fermentation broth without adding APG was used as blank control.
[0044] (3) adding polyaluminium chloride to the fermented liquid after step (2) washing carries out flocculation precipitation, and the flocculation precipitation maintenance system pH is about 6, and the add-on of polyalum...
Embodiment 3
[0050] A kind of preparation method of rhamnolipid complex surfactant of the present embodiment comprises following preparation steps:
[0051] (1) Add lysozyme to the rhamnolipid fermentation liquid, the addition of lysozyme is 0.04% of the quality of the rhamnolipid fermentation liquid, under the condition that the temperature is 45~50° C. and the pH adjusted by phosphoric acid is 5.5~6.5 The reaction was stirred for 2h.
[0052] (2) Cool the fermented liquid after the lysing treatment in step (1) to room temperature, then add APG to stir and wash, and the amount of APG added is 3.5% of the mass of the fermented liquid after the lysing process.
[0053] (3) Add polyaluminium chloride to the fermented liquid after step (2) washing and carry out flocculation precipitation, flocculation precipitation maintenance system pH is about 6, adjusts the add-on of polyaluminium chloride respectively to be 0.05 of the fermented liquid quality after washing %, 0.1%, 0.2%, 0.35%, 0.5% and...
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