Composite anti-biological inoculant as well as preparation method and application thereof

Abstract

The invention discloses a composite anti-biological inoculant prepared by compounding fermentation liquor of bacillus pumilus NF2 and fermentation liquor of paenibacillus polymyxa DL1, as well as a preparation method of the composite anti-biological inoculant and application of the inoculant in aspects of controlling pepper phytophthora blight, improving disease resistance of pepper and increasingpepper yield. The composite anti-biological inoculant combines the anti-biological characteristics of the bacillus pumilus NF2 and paenibacillus polymyxa DL1, the antibacterial spectrum is widened, multiple antibacterial mechanisms are combined, the micro-ecological balance of soil is fully coordinated, the adaptability of the strain to the environment is enhanced, the synergistic disease resistance advantages among the strains are achieved for a long time, and the composite anti-biological inoculant has an excellent anti-biological effect compared with a single strain of paenibacillus polymyxa DL1. In addition, the composite anti-biological inoculant is capable of regulating the micro-ecological environment of plant rhizosphere soil, and producing system disease resistance by plant induction, so that the plant disease resistance can be improved, the comprehensive control effects of promoting growth and resisting diseases are finally achieved, and the aims of improving the quality, increasing yield and increasing income of the pepper can be achieved.

Description

technical field [0001] The invention relates to a composite biocontrol agent and its preparation method and application, in particular to a composite biocontrol agent compounded from the fermentation broth of Brevibacillus NF2 and the fermentation broth of Paenibacillus polymyxa DL1 , as well as the preparation method of the composite biocontrol agent and its application in the prevention and treatment of pepper blight, improving the disease resistance of pepper and increasing the yield of pepper belong to the technical field of biological bacteria agent. Background technique [0002] Pepper blight, commonly known as "dead seedling disease", is a soil-borne disease caused by Phytophthora capsici Leonian. The fungus survives the winter in the soil or diseased residues in the form of oospores. Spread by other agricultural activities, new sporangia can be produced after the onset, forming zoospores for re-infection. Capsicum can be infected from the seedling stage to the adult...

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Problems solved by technology

[0004] (1) Breeding of disease-resistant varieties: Germplasm resources resistant to pepper blight are relatively scarce, and changes in the fitness of pathogenic bacteria will lead to the degradation of disease-resistant varieties, and even loss of disease resistance, so it is not conducive to long-term effects;

[0005] (1) Improvement of farming measures: Preventing pepper blight by improving agricultural farming measures is not only time-consuming and laborious, but also cannot fundamentally control the occurrence of the disease;

[0006] (3) Physical disinfection: If the operation is not done properly, it will easily lead to more serious soil-borne diseases, and it is necessary to supplement the soil with probiotics in time to achieve better control effect. However, this important step of supplementing probiotics is often easily taken by farmers. ignore

[0007] (4) Application of chemical agents: Capsicum blight is a soil-borne disease. If chemical pesticides are used to control and apply pesticides to the soil, it will cause a series of complex processes such as physical adsorption, chemical adsorption, migration and microbial degradation in the soil, which seriously affects the chemical For the control effect of pesticides, if the amount of pesticides is excessively increased, it will not only affect the growth of peppers, increase the amount of pesticide residues in pepper fruits, inhibit the activities of beneficial microorganisms in the soil, but also pollute the environment and increase production costs

[0013] However, whether Bacillus is used alone, in combination with chemical pesticides or botanical fungicides, or in combination with other antagonistic bacteria, it only shows the effect of preventing and controlling diseases, and does not play a role in promoting plant growth.

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