Bacillus safensis YZS-C03 and application thereof
A technology of YZS-C03 and Bacillus suffolus, which is applied in the field of microorganisms, can solve the problems of insufficient effective microbial resources and low treatment efficiency, and achieve the effect of great application prospects
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Embodiment 1
[0021] Example 1 Isolation, screening and identification of Bacillus shamfu
[0022] (1) Separation and screening process:
[0023] By gradient dilution method, 319 isolates were isolated from Fujian mangrove plants. Purify the isolated endophytic bacteria by the three-section line method, and judge whether the strains are purified by microscopic examination, number the purified bacteria, pick single colonies, and transfer them to NA slant medium for storage. Coat sterilized polyethylene powder 0.02g / plate (plate diameter 9cm) on the surface of the non-carbon source mineral salt solid medium plate, culture the bacterial strain obtained above by streaking on the surface, and screen the rapidly growing single colony on the NA plate cultured on culture medium. Through the growth determination of carbon-source-free mineral salt medium with polyethylene as the only carbon source, 7 strains with good polyethylene degradation effect were preliminarily screened out, and an endophyti...
Embodiment 2
[0032] Example 2 Degradation effect of Bacillus shamfu to polyethylene film
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