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Bacillus safensis, microbial agent and application of bacillus safensis and microbial agent

A technology of bacillus safflower and microbial inoculum, which is applied in the field of agricultural biology, can solve the problems of low fertilizer efficiency of phosphate fertilizer and the like, and achieve the effect of increasing fertilizer efficiency

Inactive Publication Date: 2014-07-23
烟台大境生态环境科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to overcome the defects of low fertilizer efficiency of existing phosphate fertilizers, the present invention provides a Bacillus safensis whose preservation number is CGMCC NO.8843

Method used

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  • Bacillus safensis, microbial agent and application of bacillus safensis and microbial agent
  • Bacillus safensis, microbial agent and application of bacillus safensis and microbial agent

Examples

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preparation example Construction

[0021] Wherein, the preparation method of the microbial agent may include: inoculating Bacillus suffolus of CGMCC No.8843 in the fermentation medium for culturing. Preferably, in each gram of the microbial agent obtained by culturing, the number of live bacteria of Bacillus suffolus with a preservation number of CGMCC NO.8843 can be (5-200)×10 7 CFU, more preferably (20-120)×10 7 CFU. Wherein, the cultivation temperature may be 28-35° C., and the cultivation time may be 10-50 hours.

[0022] Preferably, the preparation method of the microbial agent may also include: mixing the material obtained after culturing in the fermentation medium with an inert solid carrier and a binder; the inert solid carrier includes clay, silicon dioxide, silicon At least one of algal earth, kaolin, attapulgite, montmorillonite, bentonite, talc, white carbon black and calcium carbonate; the binder includes maltose and / or dextrin. Preferably, the amount of the inert solid carrier and the binder is...

Embodiment 1

[0029] This embodiment is used to illustrate the cultivation of Bacillus suffolus of the present invention and the preparation of microbial inoculum.

[0030] Inoculate Bacillus safensis with preservation number CGMCC NO.8843 into LB medium (containing 10 g / L of tryptone, 5 g / L of yeast extract and 10 g / L of NaCl), at 30°C and 200 rev / min shaking culture to bacterial density OD 600 The value is 0.6-0.8, and the seed bacterial liquid is obtained.

[0031] 100mL of above-mentioned seed bacterium liquid is added in 100L fermentation medium (fermentation medium contains the corn flour of 80g / L, the starch of 80g / L, the sucrose of 80g / L, the soybean oil of 40g / L, the sodium chloride of 3g / L, 3g / L of calcium carbonate, 5g / L of potassium dihydrogen phosphate and 5g / L of ferrous sulfate), cultured at 30°C, samples were taken during the culture and observed by direct counting under a microscope until each gram of culture The number of live bacteria of Bacillus shamrock in the liquid ...

Embodiment 2

[0033] This embodiment is used to illustrate the cultivation of Bacillus suffolus of the present invention and the preparation of microbial inoculum.

[0034] Inoculate Bacillus safensis with preservation number CGMCC NO.8843 into LB medium (containing 10 g / L of tryptone, 5 g / L of yeast extract and 10 g / L of NaCl), at 30°C and 200 rev / min shaking culture to bacterial density OD 600 The value is 0.6-0.8, and the seed bacterial liquid is obtained.

[0035]100mL of above-mentioned seed bacterium liquid is added in 100L fermentation medium (fermentation medium contains the corn flour of 80g / L, the starch of 80g / L, the sucrose of 80g / L, the soybean oil of 40g / L, the sodium chloride of 3g / L, 3g / L of calcium carbonate, 5g / L of potassium dihydrogen phosphate and 5g / L of ferrous sulfate), cultured at 30°C, samples were taken during the culture and observed by direct counting under a microscope until each gram of culture The number of live bacteria of Bacillus shamrock in the liquid w...

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Abstract

The invention provides bacillus safensis the preservation number of which is CGMCC NO.8843. The invention further provides a microbial agent which contains thallus and a culture medium; the thallus comprises bacillus safensis the preservation number of which is CGMCC NO.8843. The invention further provides application of the bacillus safensis and the microbial agent in preparation of a microbial fertilizer. By means of the technical scheme, the fertilizer efficiency of the phosphatic fertilizer can be increased more effectively.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a bacillus suffolus, a microbial agent and applications thereof. Background technique [0002] Phosphorus fertilizer can promote tomato flower bud differentiation, early flowering and fruiting, promote seedling root growth and improve fruit quality. When phosphorus is deficient, the young shoots and roots grow slowly, the plants are short, the leaves are dark green, dull, and the back is purple. Phosphorus is usually taken up by plants in the form of orthophosphate (hydrogen phosphate or dihydrogen phosphate). When phosphorus enters the plant, most of it becomes organic matter, and a part remains in the form of inorganic salt. Phosphorus exists in the form of phosphate in sugar phosphate, nucleic acid, nucleotide, coenzyme, phospholipid, phytic acid, etc. Phosphorus plays a key role in the reaction of ATP, and phosphorus plays an important role in carbohydrate metabol...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C05G3/00C12R1/07
Inventor 赵君才阮志勇赵秉强刘艳伟赵文勇吕昭辉刘慧宋昭王彦伟刘小飞
Owner 烟台大境生态环境科技股份有限公司
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