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A method for transforming and extracting resveratrol using bacteria

A technology of resveratrol and bacteria, applied in the field of bacterial transformation and extraction of resveratrol, can solve the problems of large amount of solvent, high production cost, non-renewable and the like

Active Publication Date: 2020-10-30
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The efficiency of extracting resveratrol from plants is low, and the amount of solvent used is large; the photothermal instability of resveratrol limits the application of chemical synthesis in the industrial production of resveratrol; enzymatic conversion of resveratrol, production The cost is high and non-renewable, so the process method needs to be continuously improved; the direct transformation method of microbial cells is rising because of its high conversion rate, environmental friendliness, and ease of separation and purification. There are few reports, but compared with mold fermentation, bacterial fermentation has the advantages of short production cycle, low cost, large bacterial concentration, high conversion efficiency, and easy control of reaction conditions, and is more suitable for production

Method used

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  • A method for transforming and extracting resveratrol using bacteria
  • A method for transforming and extracting resveratrol using bacteria
  • A method for transforming and extracting resveratrol using bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Add 0.1% substrate Polygonum cuspidatum crude medicinal material to the culture medium (pH=7.0), insert 1% Bacillus shamford seed solution (OD 600nm =1.0), at 37°C, 200r / min, shake flask fermentation for 10h, and the product has been precipitated. Centrifuge at 5000r / min, 4°C, discard the supernatant; wash the precipitate with water, centrifuge to remove the supernatant; add 100% ethanol to the precipitate, and centrifuge again at 5000r / min, 4°C, Discard the precipitate; evaporate the organic solvent in the supernatant to obtain the resveratrol product with a purity of 99.3%.

Embodiment 2

[0029] Add 0.3% substrate Polygonum cuspidatum crude drug into the culture medium (pH=7.0), insert 3% Bacillus shamfu seed solution (OD 600nm =0.8), at 28°C, 180r / min shake flask fermentation for 12h, product was precipitated. Centrifuge at 2000r / min, 25°C, discard the supernatant; wash the precipitate with water, centrifuge to remove the supernatant; add ethanol with a purity of 80% to the precipitate, and centrifuge again at 2000r / min, 25°C , discard the precipitate; evaporate the organic solvent in the supernatant to obtain the resveratrol product with a purity of 99.3%.

Embodiment 3

[0031] Add 0.1% substrate Polygonum cuspidatum crude medicinal material into the culture medium (pH=5.0), insert 3% Bacillus shamford seed solution (OD 600nm =0.8), at 37°C, 200r / min shake flask fermentation for 6h, product was precipitated. Centrifuge at 3000r / min, 25°C, discard the supernatant; wash the precipitate with water, centrifuge to remove the supernatant; add methanol with a purity of 90% to the precipitate, and centrifuge again at 3000r / min, 25°C , discard the precipitate; evaporate the organic solvent in the supernatant to obtain the resveratrol product with a purity of 99.0%.

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Abstract

The invention provides a method for converting and extracting resveratrol by means of bacteria. A fermentation medium with the addition of a substrate, namely a polygonum cuspidatum crude medicinal material, is inoculated with a bacillus safensis seed liquid, fermentation is conducted at the temperature of 28-37 DEGC for 6-15 hours, when the thalli concentration OD600nm is equal to 0.8-1.6, a product starts to be separated out, thalli, the substrate and the product are interacted, the product with the thalli as crystal nuclei is crystallized and separated out under the action of microorganisms, fermentation liquid is centrifuged, washed with distilled water and precipitated, an organic solvent is added into precipitates, one time of extraction and drying are conducted, and the resveratrol product can be obtained. The purity is larger than or equal to 99%. The method is simple in operation, mild in condition, short in conversion time and high in product yield.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to a method for transforming and extracting resveratrol by using bacteria. Background technique [0002] Resveratrol (Resveratrol) is a kind of non-flavonoid polyphenols with natural activity. 14 h 12 o 3 , is a colorless needle-like crystal, hardly soluble in water, easily soluble in organic solvents such as acetone, ethanol, methanol, ethyl acetate, etc., has poor light and thermal stability, and needs to be stored at low temperature and protected from light. [0003] Resveratrol is regarded as another new green anti-cancer drug after paclitaxel. As a phytoalexin, it is produced when plants are attacked by pathogens and the environment deteriorates. Anti-cancer, protect the cardiovascular system, lower blood sugar, promote the survival and proliferation of neural stem cells, specific or non-specific immune regulation, protect gastric mucosa, anti-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/22C07C37/68C07C39/21C12R1/07
CPCC07C37/685C12P7/22C07C39/21
Inventor 李玉胡小妍冯薇王洪彬路福平
Owner TIANJIN UNIV OF SCI & TECH
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