33 results about "Glycitein" patented technology

The invention discloses flour for diabetes food therapy. The flour is prepared with the following weight parts of raw materials: 45-55 parts of 366 wheat red dog flour, 6-8 parts of oat, 6-8 parts of glycitein, 6-8 parts of pueraria root, 6-8 parts of yam, 6-8 parts of corn silk, and 7-9 parts of pumpkin powder. The preparation method includes that the raw materials are grinded into powder and then evenly mixed. The invention can completely solve the problem that the deficiency disease of the diabetes patients is caused by excessive diet, can ensure the body to evenly absorb all nutritious ingredients, can effectively control the blood sugar and urine sugar rise of the diabetes patients, and can reduce the rate to get diabetes for ordinary people which have not got diabetes yet.

The invention discloses a method used for separating and identifying flavonoid matters in tobacco by adopting liquid chromatography-mass spectrography. The method comprises the following steps: a sample is ground and dried, an extractant is added according to the standard that 0.1 ml of the extractant is added into 1 mg of the sample for extraction and centrifugation, a supernatant liquor is collected, water and chloroform are added according to the standard that 0.5 ml of the water and 1 ml of the chloroform are added into 1 ml of the supernatant liquor for centrifugation, and the supernatant liquor is collected for analysis; 153+ and 151- are adopted as characteristic ions for scanning, parent ions are extracted for second level mass spectrum scanning, and an obtained spectrogram is compared with a spectral library to determine a chemical structure and verified through a reference substance; the molecules and ions of the identified matters and myricetin, dihydromyricetin, catechinic acid / epicatechin, daidzein, glycitein and glycyrrhizin / isoliquiritigenin are adopted as characteristic ions for scanning, the molecules and ions of the searched glycosylation flavonoid matters are used as the parent ions for the second level mass spectrum scanning, an obtained spectrogram is compared with the spectral library to determine a chemical structure and verified through the reference substance. According to the invention, the method is suitable for the analyzing and detecting micro even trace flavonoid matters.

The invention discloses a method for quickly determining six soy isoflavones in a bean product by utilizing UPLC (Ultra Performance Liquid Chromatography), and belongs to the technical field of analytical chemistry. The types of the tested bean product comprise soybean milk, bean curd, soy chicken, dried tofu, bean curds, dried beancurd sticks and the like, and the six soy isoflavones such as daidzin, isoflavoues Aglycone, Glycitin, glycitein, genistin and genistein are determined. The method comprises the following steps: firstly extracting the soy isoflavones in the bean product by using a methanol solution, after collecting a supernate fluid, repeatedly extracting residual twice by using methanol, merging the supernate fluids, and carrying out UPLC detection. Quick determination for the composition and content of the six soy isoflavones mainly in the bean product are achieved by adopting UPLC. The method is simple in sample pretreatment, high in analysis speed, high in repeatability and accuracy, and provides an efficient and accurate analytical method means for evaluating and controlling the quality of the bean product.

The invention discloses a method for separating genistein from an isoflavone genin mixture, which is characterized by efficiently separating the genistein from the isoflavone genin mixture containing glycintein, genistein and glycitein by using ionic liquid or a binary mixed solvent containing the ionic liquid and other polar solvents as an extracting agent through a fractional extraction method. The method has the advantages of high separation efficiency, little solvent consumption, low production cost, safety, environmental protection, and the like.

Methods and compositions for the prevention and / or treatment of metastatic cancer by tumor encapsulation achieved by introducing a physical obstacle to prevent cancerous cells from escaping the original site and invading other tissues. Physical obstacle is introduced as a pre-formed matter or its assembly by the treated organism is induced. In the preferred embodiment the composition of matter is disclosed, which enables formation of the obstacle in a form of basement membrane (BM). Said composition enables tissue cells to assemble BM on their surfaces, and supplies ECM components for said assembly, whereby BM is assembled de novo or the existing BM is strengthens. The composition comprises soy phytoestrogens, including genistein, daidzein, glycitein, biochantin A, and derivative thereof, actin cytoskeleton reorganization inhibitors, such as Cytochalasin D, and PTK inhibitors, in combination with ECM proteins, such as laminins and type IV collagens, or their derivatives.

The invention relates to a concentration and separation method of microcomponent in a complex system, in particular to the selective concentration and separation method of genistein in radix puerariae crude extract. The steps adopted are as follows: a) genistein molecularly imprinted polymer microspheres are employed as filler to prepare solid phase extraction column; b) the solid phase extraction column prepared in the step a is activated and sampled and then the solid phase extraction column is leached by using acetonitrile solution with water accounting for 49-51 percent of volume ratio and acetonitrile solution with water accounting for 4.9-5.1 percent of volume ratio in sequence, and then the leached solid phase extraction column is eluted by methanol solution with water accounting for 0.18-0.22 percent of volume ratio, thus obtaining finished products through drying the eluent. The invention has the advantages that: after the radix puerariae ethanol crude extract is extracted through molecularly imprinted solid phase, a clean chromatogram is obtained after removing most disturbance factor and the recovery ratio of the genistein reaches above 70 percent. Meanwhile, glycitein is reserved obviously. The invention has wide application prospect on the pretreatment of radix puerariae crude extract samples.

The present invention is a composition comprising a plant sterol and a soy protein material and / or and isoflavone selected from genistein, daidzein, glycitein, biochanin A, formononetin, and their naturally occurring glycosides, where the plant sterol comprises at least 0.49% of the composition, by weight. The present invention is also a method for decreasing the blood concentration of total and LDL cholesterol in a human in which a plant sterol and a soy protein material and / or an isoflavone are co-administered to the human, where the plant sterol comprises at least 0.49%, by weight, of the combined weight of the plant sterol and the soy protein material and / or the isoflavone. The present invention is also a method for preventing or minimizing the development of atherosclerosis in a human in which a plant sterol and a soy protein material and / or an isoflavone are co-administered to the human, where the plant sterol comprises at least 0.49%, by weight, of the combined weight of the plant sterol and the soy protein material and / or the isoflavone.

The invention discloses a soil improvement base material for planting soybeans and a preparation method thereof. The soil improvement base material is prepared from the following raw materials: winterwheat seeds, plant ash, turf, farmyard manure, pigeon manure, a yeast, soybean roots, an inorganic salt solution, a modified glycitein phospholipid compound, vermiculite, pearlite, kaolin and clean water; the preparation method comprises the following steps: 1) immersing winter wheat to be sprouted; then squeezing and stirring; taking an extracting solution for standby use; 2) adding the yeast into the plant ash, the turf, the farmyard manure and the pigeon manure and fermenting; taking a fermented material for standby use; 3) after crushing the soybean roots, adding the inorganic salt solution, the modified glycitein phospholipid compound and the extracting solution in step 1) into the fermented material of step 2); carrying out oscillation and dispersion; 4) after mixing the vermiculite, the pearlite and the kaolin, adding a mixture into step 3); mixing and stirring to obtain the soil improvement base material. According to the soil improvement base material prepared by the preparation method, a preparation technology is simple and the yield and quality of soybeans can be effectively improved; the soybeans can be fuller and the economic benefits are improved.

The invention discloses a preparation method of high aglycone isoflavone soybean milk powder, which belongs to the technical field of food processing. The method comprises the following steps of preparing the soybean milk powder by adding beta-glucosidase and a pecto-cellulose complex enzyme into soybean milk before soybean milk and bean dregs are separated and carrying out preconcentration and spray drying; the prepared soybean milk powder is normal in color and cannot be browned , content of dsupportzein, glycitein and genistin in the soybean milk powder is obviously improved, and health care value of the soybean milk powder is obviously promoted.

The invention discloses a medicine composition for improving piglet production performance. The medicine composition consists of the following components in parts by weight: 1.8-2.6 parts of daidzin, 0.1-0.3 part of glycitin, 0.8-1.5 parts of genistin, 2.1-3.7 parts of daidzein, 0.1-0.25 part of glycitein and 0.2-0.3 part of genistein. The invention further discloses a preparation method and application of the composition. The medicine composition disclosed by the invention is good in application prospect, and the production performance of piglets can be effectively improved.

This invention pertains to methods for decreasing the ratio of liquiritigenin-derived isoflavones relative to total isoflavone levels in isoflavonoid-producing plants and plant parts by transforming plants with a recombinant DNA construct comprising a nucleic acid sequence of at least 200 nucleotides and having at least 75% sequence identity to a polynucleotide encoding a chalcone reductase. More preferably, this invention pertains to methods for decreasing the ratios of daidzein and glycitein and their conjugates relative to total isoflavone levels in soybean plants and soybean plant parts by transforming plants with a recombinant DNA construct comprising a nucleic acid sequence of at least 200 nucleotides and having at least 75% sequence identity to a polynucleotide encoding a chalcone reductase.

The invention discloses a method for preparing glycitein by taking bean germ as a raw material. The method comprises the following steps: conducting extracting on 80-100-mesh bean germ powder in 55-65% ethanol for 45-60 minutes to obtain an extracting solution, wherein 500-600ml of an ethanol solution is added into every 100g of the bean germ powder, and evaporating the extracting solution to dryness to obtain dry residues; adding n-hexane into the dry residues for extraction, and then discarding an n-hexane supernate to obtain residues; and adding ethyl acetate into the residues for extraction, conducting centrifugation to obtain a supernate, and evaporating the supernate to dryness to obtain the glycitein. When the bean germ powder is subjected to extraction by a specific process, single-component isoflavone-glycitein can be extracted, and a glycitein pure product is obtained after n-hexane degreasing and impurity removal by ethyl acetate. The method is simple, rapid, low in cost andsuitable for industrial production.

The invention relates to 4-(4-(3-trifluoro methyl) benzamido phenoxyl)-2-(methyl carbamyl) pyridine salt, its preparation method and an application, which belongs to the chemical medicine field. The structure of 4-(4-(3-trifluoro methyl) benzamido phenoxyl)-2-(methyl carbamyl) pyridine salt is shown as a formula I, X can be one of hydrochloric acid, acetic acid, benzoic acid, benzene sulfonic acid, citric acid, fumaric acid, glucurnic acid, malic acid, maleate, C1-C8 alkyl substituted sulfonic acid, naphthoic acid, nitric acid, oleic acid, oxalic acid, phosphoric acid, succinic acid, tartrate, sulfuric acid, C1-C8 alkyl substituted benzene sulfonic acid, ethanolamine, trifluoroacetic acid, lactic acid, dinaphthyl salicylic acid or asccharin. 4-(4-(3-trifluoro methyl) benzamido phenoxyl)-2-(methyl carbamyl) pyridine salt of the present invention has the advantages of high solubility and high bioavailability, which can be used for preparing medicines to treat cancer.

The invention discloses a method for preparing a vegetarian dried meat floss product from cordyceps millitaris fermented glycine max dregs. A glycine max processing byproduct, namely glycine max dregs, is adopted as a raw material, through solid fermentation, flavor blending and frying with cordyceps millitaris, contents of two soy isoflavones, namely daidzein and glycitein, can be remarkably increased, the content of insoluble cellulose can be reduced, and the vegetarian dried meat floss product which is rich in aglycone isoflavones, proteins and edible fungi, is cotton-like and fluffy and has sensitive, physical and chemical and microorganism indexes similar to those of dried meat floss is prepared. The product prepared by using the method is "natural, nutrient and low-grease", and expectation of different customers on nutrient and healthy foods can be met. Solid fermentation used in the method is a traditional and modern biological technology, is efficient and low-carbon, good in applicability and simple to operate, and is free of secondary sterilization; the cordyceps millitaris can be used as an edible fungus, the mycelia of the cordyceps millitaris is rich in nutrition, and the flavor and the drawing feeling of the product can be also improved. By adopting the method, the nutrition and functional value of the vegetarian dried meat floss leisure food can be effectively intensified, and great significances in increasing additional values of the vegetarian dried meat floss product and promoting comprehensive utilization of the glycine max dregs can be achieved.

The invention belongs to the technical fields of organic chemistry and pharmaceutical synthesis and relates to a method for synthesizing glycitein and analogs thereof. The glycitein is 6-methoxy-4',7-dihydroxyl isoflavone. The method is performed according to a synthesis route represented by a formula shown in the description, the synthesized glycitein is 6-methoxy-4',7-dihydroxyl isoflavone or ananalog thereof; and the synthesis route of the method is high in yield, simple and convenient in operation and moderate in conditions and is adaptable to large-scale preparation.

The invention discloses a method for quickly measuring six types of soy isoflavones in a feed additive. The six types of soy isoflavones are respectively daidzin, glycitin, genistin, daidzein, glycitein and genistein. The method comprises the following steps of firstly, using 80% ethanol to extract the soy isoflavones in the feed additive product, centrifuging, taking a supernatant, filtering, and performing HPLC (high performance liquid chromatography) detection. The method has the advantages that the contents of six types of soy isoflavones in the feed additive product can be quickly measured; the pretreatment of a sample is simple, the analyzing speed is high, the repeatability and the accuracy are high, and an efficient and accurate analyzing method is provided for evaluating and controlling the quality of the feed additive product.

The invention discloses detection for the content of isoflavone aglycone in fermented soybean based on quantitative analysis of multiple components by single marker, which comprises the steps of (1) establishing a standard curve of genistein; (2) measuring the content of genistein in a sample to be detected; and (3) calculating the content of daidzein and glycitein in the sample to be detected byusing a relative correction factor. The quantitative analysis of multiple components by single marker and an external standard method are mutually verified, there is no obvious difference, and the result is reliable. The method disclosed by the invention calculates the content of daidzein and glycitein through measuring the content of genistein in the fermented soybean by combining the relative correction factor f and chromatographic peak location in the absence of daidzein and glycitein reference substances, thereby providing a new method for quality control of the fermented soybean.

The present invention discloses an artemisia ordosica root extract and a preparation method and an application thereof. The preparation method of the artemisia ordosica root extract comprises the following steps: drying and crushing artemisia ordosica roots and adding an ethanol solution for ultrasonic extraction; then mixing each extract and conducting concentrating and drying to obtain an extract A; and (2) dissolving the extract A with distilled water, and sequentially conducting extraction with petroleum ether, ethyl acetate and n-butanol; and collecting an ethyl acetate layer extract, andconducting concentration under reduced pressure and drying to obtain a finished product. The artemisia ordosica root extract contains 50-90% of total flavonoids, and eriodictyol, isosakuranetin, naringenin, hydroxygenkwanin, genkwanin, acacetin, diosmetin, glycitein, isorhamnetin, morin and quercetin with the total content of more than 2%. The artemisia ordosica root extract can obviously reduceallergic rhinitis symptoms of guinea pigs, also reduces levels of inflammatory factors of histamine, ovalbumin specific IgE, IL-2 / 4 / 10, IFN-gamma, ICam-1, etc. in serum of the guinea pigs, and can also reduce infiltration of eosinophilic granulocyte of nasal mucosa of nasal cavities of the guinea pigs.

The invention discloses a soybean isoflavone extract. The soybean isoflavone extract is prepared from, by weight, 1.8-10% of daidzin, 0.1-1% of soybean xanthosine, 0.8-8% of genistin, 2.1-12% of isoflavoues aglycone, 0.1-1% of glycitein and 0.2-2% of genistein. It is shown by effect experiments that productive performance of the piglets is obviously improved after the soybean isoflavone extract is added into feed to feed piglets. Compared with an existing soybean isoflavone product, the extract has remarkable advantages, indexes such as daily gain, average daily feed intake and the feed conversion ratio are higher than those of the commercially available product Meilidun on the same feeding conditions, and the extract has good market prospects in the field of livestock and poultry breeding. The invention further provides a preparing method of the soybean isoflavone extract. The preparing method is simple in extraction technology, short in extraction time, low in extraction temperature, low in energy consumption and beneficial for large-scale production and reduces production cost to the maximum, and solvents in use can be used for fast extracting special components and are small in use amount.

The invention discloses a composition for preventing kidney stone, comprising the following components in parts by weight: 10-70 parts of daidzin, 3-25 parts of genistin, 5-35 parts of glycitin, 0.1-2 parts of daidzein, 0.005-0.05 part of genistein and 0.01-0.1 part of glycitein. The total amount of the components accounts for above 80w / w% of the total weight of the composition.

The invention relates to a koji soybean using alcohol fermentating yeast and product method of the same. In the invention, non-genetically modified soybeans or wheats are subjected to the heat denaturation in a stainless steel fermentation jar, then the alcohol fermenting yeast strains are added and the fermentation is performed for 72 hours, the fermentation is stopped by adding the protease generation strains, the fermentation product is dried by hot blast, thus the health koji soybean or related product, which has increased Genistin, Daidzein and Glycitein content and improved comprehensive hobby, is obtained.

The invention relates to a concentration and separation method of microcomponent in a complex system, in particular to the selective concentration and separation method of genistein in radix puerariaecrude extract. The steps adopted are as follows: a) genistein molecularly imprinted polymer microspheres are employed as filler to prepare solid phase extraction column; b) the solid phase extractioncolumn prepared in the step a is activated and sampled and then the solid phase extraction column is leached by using acetonitrile solution with water accounting for 49-51 percent of volume ratio andacetonitrile solution with water accounting for 4.9-5.1 percent of volume ratio in sequence, and then the leached solid phase extraction column is eluted by methanol solution with water accounting for 0.18-0.22 percent of volume ratio, thus obtaining finished products through drying the eluent. The invention has the advantages that: after the radix puerariae ethanol crude extract is extracted through molecularly imprinted solid phase, a clean chromatogram is obtained after removing most disturbance factor and the recovery ratio of the genistein reaches above 70 percent. Meanwhile, glycitein is reserved obviously. The invention has wide application prospect on the pretreatment of radix puerariae crude extract samples.

The invention belongs to the field of organic pharmaceutical cocrystal technology and specifically relates to a vilazodone organic pharmaceutical cocrystal and a preparation method thereof. The pharmaceutical cocrystal uses vilazodone as an active component and glucide as a precursor. A basic structural unit of the vilazodone organic pharmaceutical cocrystal comprises four vilazodone molecules and four glucide molecules and a three-dimensional network structure of the vilazodone organic pharmaceutical cocrystal is formed by extending vilazodone molecule and glucide molecule in the direction of X, Y, and Z through hydrogen bond. Space group of the pharmaceutical cocrystal is monoclinic system with axial lengths of a=11.0269-11.1269, b=18.3006-18.4006, c=15.4106-15.4106 and axial angles of alpha=89.950 degrees-90.050 degrees, beta=100.631 degrees-100.731 degrees, gamma=89.950 degrees-90.050 degrees. According to the invention, formation of new crystalline phase of the pharmaceutical cocrystal not only retains the pharmacological properties of the drug but also provides possibilities for further increasing efficacy and bioavailability through modification of physical and chemical properties of the drug such as melting point, stability, solubility, dispersion rate and the like.

The invention discloses a preparation method of a glycitein purified product. The method comprises the steps of soybean milk is subjected to standing settling, alkali adding treatment and ethyl acetate selectivity extraction; during alkali adding treatment, the added alkali is NaOH; the soybean milk is whey discharged in the soybean protein producing process through the alkaline extraction and acid settling technology. Degreased bean pulp is subjected to alkaline extraction so that soybean fiber and other protein can be separated, the protein and soybean isoflavones can be separated, the protein can be subjected to acid settling to be separated from isoflavones in the solution, alkali extraction and acid settling discharge solutions are mixed to obtain soybean milk with the pH close to 5-6.5, and the soybean milk contains a large amount of glycitein, and is the high-quality material for preparing glycitein.

The invention discloses a method for preparing glycitein by taking bean germ as a raw material. The method comprises the following steps: conducting extracting on 80-100-mesh bean germ powder in 55-65% ethanol for 45-60 minutes to obtain an extracting solution, wherein 500-600ml of an ethanol solution is added into every 100g of the bean germ powder, and evaporating the extracting solution to dryness to obtain dry residues; adding n-hexane into the dry residues for extraction, and then discarding an n-hexane supernate to obtain residues; and adding ethyl acetate into the residues for extraction, conducting centrifugation to obtain a supernate, and evaporating the supernate to dryness to obtain the glycitein. When the bean germ powder is subjected to extraction by a specific process, single-component isoflavone-glycitein can be extracted, and a glycitein pure product is obtained after n-hexane degreasing and impurity removal by ethyl acetate. The method is simple, rapid, low in cost andsuitable for industrial production.

The purpose of the present invention is to obtain miso with good flavor containing large quantities of isoflavone compounds, which has decreased bitter taste derived from the isoflavone. The present invention relates to miso with good flavor containing large quantities of isoflavone compounds, which is produced by adding 0.1% to 5.0% (W / W) isoflavone compounds (e.g., genistin, daidzin, and glycitin, and aglycons of these compounds including genistein, daidzein, glycitein, and the like) and dried and fermented fish such as dried bonito or boiled-dried fish or a treated product thereof (e.g., a hot water extract of dried and fermented fish or an alcohol extract of dried and fermented fish) to miso, and relates to a method for producing the same.

The invention discloses a composition for preventing kidney stone, comprising the following components in parts by weight: 10-70 parts of daidzin, 3-25 parts of genistin, 5-35 parts of glycitin, 0.1-2 parts of daidzein, 0.005-0.05 part of genistein and 0.01-0.1 part of glycitein. The total amount of the components accounts for above 80w / w% of the total weight of the composition.

The invention discloses food therapy flour capable of reducing possibility of suffering from diabetes. The food therapy flour is made from the following raw materials in parts by weight: 45-55 parts of 366 wheat middling flour, 6-8 parts of oat, 6-8 parts of glycitein, 6-8 parts of radix puerariae, 6-8 parts of dioscorea opposita, 6-8 parts of corn stigma and 7-9 parts of pumpkin powder. A makingmethod of the food therapy flour comprises the following steps of grinding the above raw materials into powder, and performing uniform mixing so as to obtain the food therapy flour. According to the food therapy flour disclosed by the invention, the problem that diabetes patients can have deficiency diseases caused by excessive dieting can be thoroughly solved, the situation that human bodies cantake in comprehensive nutritive components in a balanced manner can be guaranteed, the rise of blood sugar and the rise of urine sugar of the diabetes patients can be effectively controlled, and whenordinary people that do not suffer from diabetes eat the food therapy flour, the possibility of suffering from the diabetes can be reduced.

The invention discloses a method for separating genistein from an isoflavone genin mixture, which is characterized by efficiently separating the genistein from the isoflavone genin mixture containing glycintein, genistein and glycitein by using ionic liquid or a binary mixed solvent containing the ionic liquid and other polar solvents as an extracting agent through a fractional extraction method.The method has the advantages of high separation efficiency, little solvent consumption, low production cost, safety, environmental protection, and the like.