33 results about "Glycitein" patented technology

The invention relates to a concentration and separation method of microcomponent in a complex system, in particular to the selective concentration and separation method of genistein in radix puerariaecrude extract. The steps adopted are as follows: a) genistein molecularly imprinted polymer microspheres are employed as filler to prepare solid phase extraction column; b) the solid phase extractioncolumn prepared in the step a is activated and sampled and then the solid phase extraction column is leached by using acetonitrile solution with water accounting for 49-51 percent of volume ratio andacetonitrile solution with water accounting for 4.9-5.1 percent of volume ratio in sequence, and then the leached solid phase extraction column is eluted by methanol solution with water accounting for 0.18-0.22 percent of volume ratio, thus obtaining finished products through drying the eluent. The invention has the advantages that: after the radix puerariae ethanol crude extract is extracted through molecularly imprinted solid phase, a clean chromatogram is obtained after removing most disturbance factor and the recovery ratio of the genistein reaches above 70 percent. Meanwhile, glycitein is reserved obviously. The invention has wide application prospect on the pretreatment of radix puerariae crude extract samples.

The invention discloses a method for preparing glycitein by taking bean germ as a raw material. The method comprises the following steps: conducting extracting on 80-100-mesh bean germ powder in 55-65% ethanol for 45-60 minutes to obtain an extracting solution, wherein 500-600ml of an ethanol solution is added into every 100g of the bean germ powder, and evaporating the extracting solution to dryness to obtain dry residues; adding n-hexane into the dry residues for extraction, and then discarding an n-hexane supernate to obtain residues; and adding ethyl acetate into the residues for extraction, conducting centrifugation to obtain a supernate, and evaporating the supernate to dryness to obtain the glycitein. When the bean germ powder is subjected to extraction by a specific process, single-component isoflavone-glycitein can be extracted, and a glycitein pure product is obtained after n-hexane degreasing and impurity removal by ethyl acetate. The method is simple, rapid, low in cost andsuitable for industrial production.

The invention discloses a method for preparing glycitein by treating primary scum of soybean milk. The method comprises the following steps of adding a Na2CO3 solution and ethanol into primary scum dry powder obtained by treating the soybean milk with polyaluminum chloride and polyacrylamide, performing stirring and extracting for set time, and conducting filtering to obtain a filtrate; evaporating the filtrate till the filtrate is dried, extracting and purifying the filtrate with ethyl acetate, and then evaporating the filtrate till the filtrate is totally dried to obtain glycitein. If a certain concentration of a mixed liquid of the Na2CO3 aqueous solution and ethanol is used for extracting the primary scum obtained by treating the soybean milk, glycitein in the primary scum can be selectively extracted, wherein the prepared glycitein has higher purity and does not contain other soybean isoflavones.

The invention discloses a composition for preventing kidney stone, comprising the following components in parts by weight: 10-70 parts of daidzin, 3-25 parts of genistin, 5-35 parts of glycitin, 0.1-2 parts of daidzein, 0.005-0.05 part of genistein and 0.01-0.1 part of glycitein. The total amount of the components accounts for above 80w / w% of the total weight of the composition.

The invention discloses a method for separating genistein from an isoflavone genin mixture, which is characterized by efficiently separating the genistein from the isoflavone genin mixture containing glycintein, genistein and glycitein by using ionic liquid or a binary mixed solvent containing the ionic liquid and other polar solvents as an extracting agent through a fractional extraction method.The method has the advantages of high separation efficiency, little solvent consumption, low production cost, safety, environmental protection, and the like.